Expression of spinach plastocyanin in E. coli

An expression vector designed for overexpression of plastocyanin in the periplasmic space of E. coli has been developed. The vector contains the signal peptide sequence of Pseudomonas aeruginosa azurin and the mature sequence of spinach plastocyanin. The precursor is efficiently translocated to the...

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Veröffentlicht in:	FEBS letters 1990-12, Vol.276 (1), p.98-102
Hauptverfasser:	Nordling, Margareta, Olausson, Torbjörn, Lundberg, Lennart G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences Biotechnology Blue-copper CD, circular dichroism Cloning, Molecular - methods E. coli Electron Spin Resonance Spectroscopy Electrophoresis, Polyacrylamide Gel EPR, electronic paramagnetic resonance Escherichia coli - genetics Expression Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors IPTG, isopropyl-β-D-thiogalactopyranoside Methods. Procedures. Technologies Molecular Sequence Data Molecular Weight Photosynthesis Plants - genetics Plasmids Plastocyanin Plastocyanin - genetics Plastocyanin - isolation & purification RBS, ribosome binding site Recombinant Proteins - isolation & purification SDS-PAGE, sodium dodecyl sulphate-poly-acrylamide gel electrophoresis Vectors (cloning, transfer, expression). Insertion sequences and transposons
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