Selective binding of mouse and human spermatozoa to beads coated with extracellular matrix components
The ability of mouse epididymal and human ejaculated spermatozoa to bind to beads coated with various extracellular matrix components was examined. Mouse spermatozoa preferentially bound to beads coated with heparin (average values ranging between 6.2 and 8.8 sperm per bead were obtained in differen...
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| Veröffentlicht in: | Molecular reproduction and development 1990-12, Vol.27 (4), p.337-343 |
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| creator | De Felici, Massimo Camaioni, Antonella Russo, Mario A. Siracusa, Gregorio |
| description | The ability of mouse epididymal and human ejaculated spermatozoa to bind to beads coated with various extracellular matrix components was examined. Mouse spermatozoa preferentially bound to beads coated with heparin (average values ranging between 6.2 and 8.8 sperm per bead were obtained in different experiments) and with chondroitinsulfate (6.2–7.0), and also, although with significant differences across replicate experiments, to beads coated with laminin (7.9–15.6 sperm per bead) and with collagen type I (6.1–18.5). Human spermatozoa bound to collagen‐coated beads (15.4–22.6 sperm per bead) and, to a much lower extent, to chondroitinsulfate‐coated beads (3.2–4.7); they were also able to bind heparin‐coated beads, although with ample differences between individual sperm donors (ranging between 0.8 and 18.7 sperm per bead). Very few human and mouse sperm bound fibronectin‐coated beads; beads coated with albumin, hyaluronic acid, and chondronectin were always totally free of adhering sperm. The possible physiological role of the interactions between spermatozoa and extracellular matrix components are discussed. |
| doi_str_mv | 10.1002/mrd.1080270408 |
| format | Article |
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Mouse spermatozoa preferentially bound to beads coated with heparin (average values ranging between 6.2 and 8.8 sperm per bead were obtained in different experiments) and with chondroitinsulfate (6.2–7.0), and also, although with significant differences across replicate experiments, to beads coated with laminin (7.9–15.6 sperm per bead) and with collagen type I (6.1–18.5). Human spermatozoa bound to collagen‐coated beads (15.4–22.6 sperm per bead) and, to a much lower extent, to chondroitinsulfate‐coated beads (3.2–4.7); they were also able to bind heparin‐coated beads, although with ample differences between individual sperm donors (ranging between 0.8 and 18.7 sperm per bead). Very few human and mouse sperm bound fibronectin‐coated beads; beads coated with albumin, hyaluronic acid, and chondronectin were always totally free of adhering sperm. The possible physiological role of the interactions between spermatozoa and extracellular matrix components are discussed.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.1080270408</identifier><identifier>PMID: 2124812</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Chondroitin Sulfates - metabolism ; Chondroitinsulfate ; Collagen ; Collagen - metabolism ; Extracellular Matrix - metabolism ; Fibronectin ; Fibronectins - metabolism ; Fundamental and applied biological sciences. Psychology ; Glycoproteins ; Heparin ; Heparin - metabolism ; Humans ; Laminin ; Laminin - metabolism ; Male ; Mammalian male genital system ; Mice ; Molecular Sequence Data ; Morphology. Physiology ; Proteins - metabolism ; Spermatozoa - metabolism ; Vertebrates: reproduction</subject><ispartof>Molecular reproduction and development, 1990-12, Vol.27 (4), p.337-343</ispartof><rights>Copyright © 1990 Wiley‐Liss, Inc.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4088-735c6ab68c8c0d907a5d950182abbd95763f9c14b5704584d47d540800cce62c3</citedby><cites>FETCH-LOGICAL-c4088-735c6ab68c8c0d907a5d950182abbd95763f9c14b5704584d47d540800cce62c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.1080270408$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.1080270408$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19532784$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2124812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Felici, Massimo</creatorcontrib><creatorcontrib>Camaioni, Antonella</creatorcontrib><creatorcontrib>Russo, Mario A.</creatorcontrib><creatorcontrib>Siracusa, Gregorio</creatorcontrib><title>Selective binding of mouse and human spermatozoa to beads coated with extracellular matrix components</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>The ability of mouse epididymal and human ejaculated spermatozoa to bind to beads coated with various extracellular matrix components was examined. Mouse spermatozoa preferentially bound to beads coated with heparin (average values ranging between 6.2 and 8.8 sperm per bead were obtained in different experiments) and with chondroitinsulfate (6.2–7.0), and also, although with significant differences across replicate experiments, to beads coated with laminin (7.9–15.6 sperm per bead) and with collagen type I (6.1–18.5). Human spermatozoa bound to collagen‐coated beads (15.4–22.6 sperm per bead) and, to a much lower extent, to chondroitinsulfate‐coated beads (3.2–4.7); they were also able to bind heparin‐coated beads, although with ample differences between individual sperm donors (ranging between 0.8 and 18.7 sperm per bead). Very few human and mouse sperm bound fibronectin‐coated beads; beads coated with albumin, hyaluronic acid, and chondronectin were always totally free of adhering sperm. The possible physiological role of the interactions between spermatozoa and extracellular matrix components are discussed.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chondroitin Sulfates - metabolism</subject><subject>Chondroitinsulfate</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibronectin</subject><subject>Fibronectins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins</subject><subject>Heparin</subject><subject>Heparin - metabolism</subject><subject>Humans</subject><subject>Laminin</subject><subject>Laminin - metabolism</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>Proteins - metabolism</subject><subject>Spermatozoa - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv1DAQhSMEKqVw5YbkC9xSxk4c20dUoEWUgiiovVmOPaGGJF5sp93y6_FqV604cfJI873nN6-qnlM4pADs9RRdGSQwAS3IB9U-BSVrJhR_uJlbqFvOLh9XT1L6CQBKSdir9hhlraRsv8JzHNFmf42k97Pz8w8SBjKFJSExsyNXy2RmklYYJ5PDn2BIDqRH4xKxwWR05MbnK4LrHI3FcVxGE0lBo18XYFqFGeecnlaPBjMmfLZ7D6rv7999OzqpTz8ffzh6c1rbkl3WouG2M30nrbTgFAjDneJAJTN9XybRNYOytO15OZbL1rXC8aIEsBY7ZpuD6tXWdxXD7wVT1pNPm1hmxnKSlsVLKugKeLgFbQwpRRz0KvrJxFtNQW961aVXfd9rEbzYOS_9hO4O3xVZ9i93e5OsGYdoZuvTvaviDROyLZzacjd-xNv__Ko_fX37T4Z6q_Up4_pOa-Iv3YlGcH1xdqwvLiX9-OW802fNX9pWoS4</recordid><startdate>199012</startdate><enddate>199012</enddate><creator>De Felici, Massimo</creator><creator>Camaioni, Antonella</creator><creator>Russo, Mario A.</creator><creator>Siracusa, Gregorio</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199012</creationdate><title>Selective binding of mouse and human spermatozoa to beads coated with extracellular matrix components</title><author>De Felici, Massimo ; Camaioni, Antonella ; Russo, Mario A. ; Siracusa, Gregorio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4088-735c6ab68c8c0d907a5d950182abbd95763f9c14b5704584d47d540800cce62c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chondroitin Sulfates - metabolism</topic><topic>Chondroitinsulfate</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibronectin</topic><topic>Fibronectins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins</topic><topic>Heparin</topic><topic>Heparin - metabolism</topic><topic>Humans</topic><topic>Laminin</topic><topic>Laminin - metabolism</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>Proteins - metabolism</topic><topic>Spermatozoa - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Felici, Massimo</creatorcontrib><creatorcontrib>Camaioni, Antonella</creatorcontrib><creatorcontrib>Russo, Mario A.</creatorcontrib><creatorcontrib>Siracusa, Gregorio</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Felici, Massimo</au><au>Camaioni, Antonella</au><au>Russo, Mario A.</au><au>Siracusa, Gregorio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective binding of mouse and human spermatozoa to beads coated with extracellular matrix components</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1990-12</date><risdate>1990</risdate><volume>27</volume><issue>4</issue><spage>337</spage><epage>343</epage><pages>337-343</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>The ability of mouse epididymal and human ejaculated spermatozoa to bind to beads coated with various extracellular matrix components was examined. Mouse spermatozoa preferentially bound to beads coated with heparin (average values ranging between 6.2 and 8.8 sperm per bead were obtained in different experiments) and with chondroitinsulfate (6.2–7.0), and also, although with significant differences across replicate experiments, to beads coated with laminin (7.9–15.6 sperm per bead) and with collagen type I (6.1–18.5). Human spermatozoa bound to collagen‐coated beads (15.4–22.6 sperm per bead) and, to a much lower extent, to chondroitinsulfate‐coated beads (3.2–4.7); they were also able to bind heparin‐coated beads, although with ample differences between individual sperm donors (ranging between 0.8 and 18.7 sperm per bead). Very few human and mouse sperm bound fibronectin‐coated beads; beads coated with albumin, hyaluronic acid, and chondronectin were always totally free of adhering sperm. The possible physiological role of the interactions between spermatozoa and extracellular matrix components are discussed.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2124812</pmid><doi>10.1002/mrd.1080270408</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Biological and medical sciences Chondroitin Sulfates - metabolism Chondroitinsulfate Collagen Collagen - metabolism Extracellular Matrix - metabolism Fibronectin Fibronectins - metabolism Fundamental and applied biological sciences. Psychology Glycoproteins Heparin Heparin - metabolism Humans Laminin Laminin - metabolism Male Mammalian male genital system Mice Molecular Sequence Data Morphology. Physiology Proteins - metabolism Spermatozoa - metabolism Vertebrates: reproduction |
| title | Selective binding of mouse and human spermatozoa to beads coated with extracellular matrix components |
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