Functional characterization of active transport of progesterone to adrenal cells
ABSTRACT The characterization of the transport mechanism of progesterone, which is one of the neutral steroids in the adrenal cells, has been studied by the examination of progesterone uptake into the monolayers of SW‐13 cells (a human adrenal adenocarcinoma cell line). The uptake of [3H]progesteron...
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| Veröffentlicht in: | Journal of pharmacy and pharmacology 2004-01, Vol.56 (1), p.79-84 |
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| container_title | Journal of pharmacy and pharmacology |
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| creator | Ogihara, Takuo Matsumoto, Shigeki Ohnishi, Shuhei |
| description | ABSTRACT
The characterization of the transport mechanism of progesterone, which is one of the neutral steroids in the adrenal cells, has been studied by the examination of progesterone uptake into the monolayers of SW‐13 cells (a human adrenal adenocarcinoma cell line). The uptake of [3H]progesterone at a tracer concentration (1 nM) exhibited temperature, pH and sodium dependency. According to kinetic analysis of the concentration dependence, the uptake of progesterone involves saturable and non‐saturable processes. The uptake for the saturable process, which gave Kt values (half‐saturation concentration) of 4.7 ± 8.7 μM, was inhibited by metabolic inhibitors and amino‐acid modifiers but not by endocytosis inhibitors or substrates for known transporters. The uptake of progesterone was also inhibited by several neutral steroids but not by anionic steroids. The inhibition by both β‐estradiol and estriol was competitive. The uptake of progesterone by the adrenal cells might be at least partially accounted for by a specific carrier‐mediated transport mechanism generated by sodium ions and an electrochemical mechanism. |
| doi_str_mv | 10.1211/0022357022386 |
| format | Article |
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The characterization of the transport mechanism of progesterone, which is one of the neutral steroids in the adrenal cells, has been studied by the examination of progesterone uptake into the monolayers of SW‐13 cells (a human adrenal adenocarcinoma cell line). The uptake of [3H]progesterone at a tracer concentration (1 nM) exhibited temperature, pH and sodium dependency. According to kinetic analysis of the concentration dependence, the uptake of progesterone involves saturable and non‐saturable processes. The uptake for the saturable process, which gave Kt values (half‐saturation concentration) of 4.7 ± 8.7 μM, was inhibited by metabolic inhibitors and amino‐acid modifiers but not by endocytosis inhibitors or substrates for known transporters. The uptake of progesterone was also inhibited by several neutral steroids but not by anionic steroids. The inhibition by both β‐estradiol and estriol was competitive. The uptake of progesterone by the adrenal cells might be at least partially accounted for by a specific carrier‐mediated transport mechanism generated by sodium ions and an electrochemical mechanism.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1211/0022357022386</identifier><identifier>PMID: 14980004</identifier><identifier>CODEN: JPPMAB</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adenocarcinoma ; Adrenal Gland Neoplasms ; Adrenal Glands - cytology ; Adrenal Glands - metabolism ; Biological and medical sciences ; Biological Transport, Active ; Cell Line, Tumor ; Gonadal Steroid Hormones - pharmacology ; Humans ; Hydrogen-Ion Concentration ; Medical sciences ; Pharmacology. Drug treatments ; Progesterone - metabolism ; Sodium ; Temperature</subject><ispartof>Journal of pharmacy and pharmacology, 2004-01, Vol.56 (1), p.79-84</ispartof><rights>2004 Royal Pharmaceutical Society of Great Britain</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5060-bb73712c42250877706bddc201d75f8419b25bff6b4a92127e7f50d2ad26c7d23</citedby><cites>FETCH-LOGICAL-c5060-bb73712c42250877706bddc201d75f8419b25bff6b4a92127e7f50d2ad26c7d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1211%2F0022357022386$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1211%2F0022357022386$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15417408$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14980004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ogihara, Takuo</creatorcontrib><creatorcontrib>Matsumoto, Shigeki</creatorcontrib><creatorcontrib>Ohnishi, Shuhei</creatorcontrib><title>Functional characterization of active transport of progesterone to adrenal cells</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>ABSTRACT
The characterization of the transport mechanism of progesterone, which is one of the neutral steroids in the adrenal cells, has been studied by the examination of progesterone uptake into the monolayers of SW‐13 cells (a human adrenal adenocarcinoma cell line). The uptake of [3H]progesterone at a tracer concentration (1 nM) exhibited temperature, pH and sodium dependency. According to kinetic analysis of the concentration dependence, the uptake of progesterone involves saturable and non‐saturable processes. The uptake for the saturable process, which gave Kt values (half‐saturation concentration) of 4.7 ± 8.7 μM, was inhibited by metabolic inhibitors and amino‐acid modifiers but not by endocytosis inhibitors or substrates for known transporters. The uptake of progesterone was also inhibited by several neutral steroids but not by anionic steroids. The inhibition by both β‐estradiol and estriol was competitive. The uptake of progesterone by the adrenal cells might be at least partially accounted for by a specific carrier‐mediated transport mechanism generated by sodium ions and an electrochemical mechanism.</description><subject>Adenocarcinoma</subject><subject>Adrenal Gland Neoplasms</subject><subject>Adrenal Glands - cytology</subject><subject>Adrenal Glands - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biological Transport, Active</subject><subject>Cell Line, Tumor</subject><subject>Gonadal Steroid Hormones - pharmacology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Progesterone - metabolism</subject><subject>Sodium</subject><subject>Temperature</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kElPwzAQhS0EgrIcuaJc4BYYT7z1iBC0LIIeQEhcLMdxIJAmxW7Zfj0OrVguXGzNvG9mnh4h2xT2KVJ6AICYcdm9SiyRHgLDVFKulkmv09IoZmtkPYRHAJBCiFWyRllfxYr1yOhk1thp1TamTuyD8cZOna8-TNdK2jKJdfXikqk3TZi0ftr1Jr69dyFybROVNjGFd1_zrq7DJlkpTR3c1uLfIDcnx9dHw_TianB6dHiRWg4C0jyXmaRoGSIHJaUEkReFRaCF5KVitJ8jz8tS5Mz0kaJ0suRQoClQWFlgtkH25nujm-dZtKPHVegcmMa1s6AVUMUYhwimc9D6NgTvSj3x1dj4d01BdxHqPxFGfmexeJaPXfFDLzKLwO4CMMGauozR2Cr8cJxRyUBFLptzr1Xt3v-_qs9GwxFy9ctuFRN--54y_kmLGBnXt5cDjbcDuB6KO32efQLucZXd</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Ogihara, Takuo</creator><creator>Matsumoto, Shigeki</creator><creator>Ohnishi, Shuhei</creator><general>Blackwell Publishing Ltd</general><general>Pharmaceutical Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200401</creationdate><title>Functional characterization of active transport of progesterone to adrenal cells</title><author>Ogihara, Takuo ; Matsumoto, Shigeki ; Ohnishi, Shuhei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5060-bb73712c42250877706bddc201d75f8419b25bff6b4a92127e7f50d2ad26c7d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adenocarcinoma</topic><topic>Adrenal Gland Neoplasms</topic><topic>Adrenal Glands - cytology</topic><topic>Adrenal Glands - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biological Transport, Active</topic><topic>Cell Line, Tumor</topic><topic>Gonadal Steroid Hormones - pharmacology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Progesterone - metabolism</topic><topic>Sodium</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ogihara, Takuo</creatorcontrib><creatorcontrib>Matsumoto, Shigeki</creatorcontrib><creatorcontrib>Ohnishi, Shuhei</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogihara, Takuo</au><au>Matsumoto, Shigeki</au><au>Ohnishi, Shuhei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional characterization of active transport of progesterone to adrenal cells</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>2004-01</date><risdate>2004</risdate><volume>56</volume><issue>1</issue><spage>79</spage><epage>84</epage><pages>79-84</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><coden>JPPMAB</coden><abstract>ABSTRACT
The characterization of the transport mechanism of progesterone, which is one of the neutral steroids in the adrenal cells, has been studied by the examination of progesterone uptake into the monolayers of SW‐13 cells (a human adrenal adenocarcinoma cell line). The uptake of [3H]progesterone at a tracer concentration (1 nM) exhibited temperature, pH and sodium dependency. According to kinetic analysis of the concentration dependence, the uptake of progesterone involves saturable and non‐saturable processes. The uptake for the saturable process, which gave Kt values (half‐saturation concentration) of 4.7 ± 8.7 μM, was inhibited by metabolic inhibitors and amino‐acid modifiers but not by endocytosis inhibitors or substrates for known transporters. The uptake of progesterone was also inhibited by several neutral steroids but not by anionic steroids. The inhibition by both β‐estradiol and estriol was competitive. The uptake of progesterone by the adrenal cells might be at least partially accounted for by a specific carrier‐mediated transport mechanism generated by sodium ions and an electrochemical mechanism.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>14980004</pmid><doi>10.1211/0022357022386</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of pharmacy and pharmacology, 2004-01, Vol.56 (1), p.79-84 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adenocarcinoma Adrenal Gland Neoplasms Adrenal Glands - cytology Adrenal Glands - metabolism Biological and medical sciences Biological Transport, Active Cell Line, Tumor Gonadal Steroid Hormones - pharmacology Humans Hydrogen-Ion Concentration Medical sciences Pharmacology. Drug treatments Progesterone - metabolism Sodium Temperature |
| title | Functional characterization of active transport of progesterone to adrenal cells |
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