Separation of phenylthiohydantoin amino acids by capillary electrochromatography

Capillary electrochromatography (CEC) was employed as a rapid and high‐efficiency method for the isocratic separation of all 20 important phenylthiohydantoin (PTH) amino acids, the end products of Edman degradation during N‐terminal protein sequencing. For this purpose, 75 μm ID fused‐silica capilla...

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Veröffentlicht in:	Electrophoresis 2004-02, Vol.25 (4-5), p.578-585
Hauptverfasser:	Mueller, Thomas, Posch, Ulrike C., Lindner, Herbert H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitriles - chemistry Amino Acids - chemistry Amino Acids - isolation & purification Buffers Capillary electrochromatography Chromatography, High Pressure Liquid Chromatography, Micellar Electrokinetic Capillary - methods Furans - chemistry Hydrogen-Ion Concentration Isocratic separation Osmolar Concentration Phenylthiohydantoin - chemistry Phenylthiohydantoin - isolation & purification Phenylthiohydantoin amino acids Protein sequencing Proteins - chemistry Reproducibility of Results Silicon Dioxide Temperature Time
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