Identification of the Coxiella Sp. Detected from Haemaphysalis longicornis Ticks in Korea

Two Haemaphysalis longicornis ticks were found positive in PCR assay of com‐1 gene to detect Coxiella burnetii DNA from 100 ticks. The nucleotide sequences of com‐1 and 16S rRNA gene were determined from 2 ticks and compared with those of other C. burnetii strains. The results suggest that H. longic...

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Veröffentlicht in:Microbiology and immunology 2004-01, Vol.48 (2), p.125-130
Hauptverfasser: Lee, Jung-Hee, Park, Hyo-Soon, Jang, Won-Jong, Koh, Seong-Eun, Park, Tae-Kyu, Kang, Shin-Seok, Kim, Bum-Joon, Kook, Yoon-Hoh, Park, Kyung-Hee, Lee, Seung-Hyun
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container_issue 2
container_start_page 125
container_title Microbiology and immunology
container_volume 48
creator Lee, Jung-Hee
Park, Hyo-Soon
Jang, Won-Jong
Koh, Seong-Eun
Park, Tae-Kyu
Kang, Shin-Seok
Kim, Bum-Joon
Kook, Yoon-Hoh
Park, Kyung-Hee
Lee, Seung-Hyun
description Two Haemaphysalis longicornis ticks were found positive in PCR assay of com‐1 gene to detect Coxiella burnetii DNA from 100 ticks. The nucleotide sequences of com‐1 and 16S rRNA gene were determined from 2 ticks and compared with those of other C. burnetii strains. The results suggest that H. longicornis harbor Coxiella sp. bacteria in Korea. Furthermore, icd, cbhE′, and cbbE′ genes are C. burnetii specific genes whereas com‐1 gene is Coxiella genus specific gene. This study gives the first documentation to prove the existence of Coxiella sp. in tick collected in Korea.
doi_str_mv 10.1111/j.1348-0421.2004.tb03498.x
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subjects 16S rRNA gene
Animals
com-1 gene
Coxiella - classification
Coxiella - genetics
Coxiella - isolation & purification
Coxiella burnetii
coxiella sp
DNA, Bacterial - analysis
Haemaphysalis longicornis
identification
Ixodidae - microbiology
Korea
Phylogeny
RNA, Ribosomal, 16S - analysis
title Identification of the Coxiella Sp. Detected from Haemaphysalis longicornis Ticks in Korea
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