Fluorescence in situ hybridization performed on exfoliated urothelial cells in patients with transitional cell carcinoma of the bladder
To evaluate comparatively fluorescence in situ hybridization (FISH) and cytology performed on exfoliated urothelial cells obtained from voided urine and bladder washings as a method of diagnosis and follow-up in patients with transitional cell carcinoma (TCC) of the bladder. Thirty patients with con...
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| Veröffentlicht in: | Urology (Ridgewood, N.J.) N.J.), 2004-02, Vol.63 (2), p.398-401 |
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| creator | Degtyar, Pinhas Neulander, Endre Zirkin, Howard Yusim, Igor Douvdevani, Amos Mermershtain, Wilmosh Kaneti, Jacob Manor, Ester |
| description | To evaluate comparatively fluorescence in situ hybridization (FISH) and cytology performed on exfoliated urothelial cells obtained from voided urine and bladder washings as a method of diagnosis and follow-up in patients with transitional cell carcinoma (TCC) of the bladder.
Thirty patients with confirmed bladder TCC, 10 patients enrolled in cystoscopy follow-up for previous bladder tumors, and 10 patients with bladders free of tumor without a previous history of bladder TCC underwent cytologic examination and FISH performed on voided urine and bladder washing specimens. The FISH probes were targeted to chromosomes 7 and 9.
FISH had a sensitivity of 92% for high-grade tumors in both voided urine and bladder washing specimens, significantly greater than that of cytology at a sensitivity of 64% in voided urine and 67% in the bladder washing specimens (
P = 0.02). The sensitivity of FISH and cytology were both low and not significantly different statistically from each other for the low-grade tumors. Monosomy of chromosome 9 correlated with early tumor recurrence. Polysomy of chromosomes 7 and 9 correlated with high-grade tumors (80% and 92%, respectively).
According to our results, with the local cytopathology expertise, FISH performed on urothelial cells from voided urine has a sensitivity that supersedes that of cytology, making the former a valuable complementary method in the diagnosis and follow-up of patients with bladder TCC. |
| doi_str_mv | 10.1016/j.urology.2003.08.026 |
| format | Article |
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Thirty patients with confirmed bladder TCC, 10 patients enrolled in cystoscopy follow-up for previous bladder tumors, and 10 patients with bladders free of tumor without a previous history of bladder TCC underwent cytologic examination and FISH performed on voided urine and bladder washing specimens. The FISH probes were targeted to chromosomes 7 and 9.
FISH had a sensitivity of 92% for high-grade tumors in both voided urine and bladder washing specimens, significantly greater than that of cytology at a sensitivity of 64% in voided urine and 67% in the bladder washing specimens (
P = 0.02). The sensitivity of FISH and cytology were both low and not significantly different statistically from each other for the low-grade tumors. Monosomy of chromosome 9 correlated with early tumor recurrence. Polysomy of chromosomes 7 and 9 correlated with high-grade tumors (80% and 92%, respectively).
According to our results, with the local cytopathology expertise, FISH performed on urothelial cells from voided urine has a sensitivity that supersedes that of cytology, making the former a valuable complementary method in the diagnosis and follow-up of patients with bladder TCC.</description><identifier>ISSN: 0090-4295</identifier><identifier>EISSN: 1527-9995</identifier><identifier>DOI: 10.1016/j.urology.2003.08.026</identifier><identifier>PMID: 14972510</identifier><identifier>CODEN: URGYAZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Aneuploidy ; Biological and medical sciences ; Carcinoma, Transitional Cell - diagnosis ; Carcinoma, Transitional Cell - genetics ; Carcinoma, Transitional Cell - pathology ; Carcinoma, Transitional Cell - surgery ; Carcinoma, Transitional Cell - urine ; Chromosomes, Human, Pair 7 ; Chromosomes, Human, Pair 9 ; Female ; Follow-Up Studies ; Humans ; In Situ Hybridization, Fluorescence ; Male ; Medical sciences ; Middle Aged ; Neoplasm Recurrence, Local - diagnosis ; Nephrology. Urinary tract diseases ; Prognosis ; Sensitivity and Specificity ; Therapeutic Irrigation ; Urinary Bladder Neoplasms - diagnosis ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology ; Urinary Bladder Neoplasms - surgery ; Urinary Bladder Neoplasms - urine ; Urine - cytology ; Urothelium - ultrastructure</subject><ispartof>Urology (Ridgewood, N.J.), 2004-02, Vol.63 (2), p.398-401</ispartof><rights>2004 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-8528528e14004be8d1d8518d00ad12186e2cbc41d5e41c4acceba898509f0dac3</citedby><cites>FETCH-LOGICAL-c391t-8528528e14004be8d1d8518d00ad12186e2cbc41d5e41c4acceba898509f0dac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0090429503009154$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15547310$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14972510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Degtyar, Pinhas</creatorcontrib><creatorcontrib>Neulander, Endre</creatorcontrib><creatorcontrib>Zirkin, Howard</creatorcontrib><creatorcontrib>Yusim, Igor</creatorcontrib><creatorcontrib>Douvdevani, Amos</creatorcontrib><creatorcontrib>Mermershtain, Wilmosh</creatorcontrib><creatorcontrib>Kaneti, Jacob</creatorcontrib><creatorcontrib>Manor, Ester</creatorcontrib><title>Fluorescence in situ hybridization performed on exfoliated urothelial cells in patients with transitional cell carcinoma of the bladder</title><title>Urology (Ridgewood, N.J.)</title><addtitle>Urology</addtitle><description>To evaluate comparatively fluorescence in situ hybridization (FISH) and cytology performed on exfoliated urothelial cells obtained from voided urine and bladder washings as a method of diagnosis and follow-up in patients with transitional cell carcinoma (TCC) of the bladder.
Thirty patients with confirmed bladder TCC, 10 patients enrolled in cystoscopy follow-up for previous bladder tumors, and 10 patients with bladders free of tumor without a previous history of bladder TCC underwent cytologic examination and FISH performed on voided urine and bladder washing specimens. The FISH probes were targeted to chromosomes 7 and 9.
FISH had a sensitivity of 92% for high-grade tumors in both voided urine and bladder washing specimens, significantly greater than that of cytology at a sensitivity of 64% in voided urine and 67% in the bladder washing specimens (
P = 0.02). The sensitivity of FISH and cytology were both low and not significantly different statistically from each other for the low-grade tumors. Monosomy of chromosome 9 correlated with early tumor recurrence. Polysomy of chromosomes 7 and 9 correlated with high-grade tumors (80% and 92%, respectively).
According to our results, with the local cytopathology expertise, FISH performed on urothelial cells from voided urine has a sensitivity that supersedes that of cytology, making the former a valuable complementary method in the diagnosis and follow-up of patients with bladder TCC.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Aneuploidy</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Transitional Cell - diagnosis</subject><subject>Carcinoma, Transitional Cell - genetics</subject><subject>Carcinoma, Transitional Cell - pathology</subject><subject>Carcinoma, Transitional Cell - surgery</subject><subject>Carcinoma, Transitional Cell - urine</subject><subject>Chromosomes, Human, Pair 7</subject><subject>Chromosomes, Human, Pair 9</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Recurrence, Local - diagnosis</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Prognosis</subject><subject>Sensitivity and Specificity</subject><subject>Therapeutic Irrigation</subject><subject>Urinary Bladder Neoplasms - diagnosis</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - pathology</subject><subject>Urinary Bladder Neoplasms - surgery</subject><subject>Urinary Bladder Neoplasms - urine</subject><subject>Urine - cytology</subject><subject>Urothelium - ultrastructure</subject><issn>0090-4295</issn><issn>1527-9995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVGL1DAUhYO4uOPqT1Dyom_t3nSaNnkSWVwVFnzR55Amt06GTDMmqe7sH9i_beoU9lEIXC585-ZwDiFvGNQMWHe9r-cYfPh5qhuAbQ2ihqZ7RjaMN30lpeTPyQZAQtU2kl-SlyntAaDruv4FuWSt7BvOYEMeb_0cIiaDk0HqJppcnunuNERn3YPOLkz0iHEM8YCWlgXvx-CdzmUrBvIOy-KpQe_TIj8WCU450T8u72iOeioHy5GVoUZH46Zw0DSMtKjp4LW1GF-Ri1H7hK_XeUV-3H76fvOluvv2-evNx7vKbCXLleDN8pC1AO2AwjIrOBMWQFvWMNFhYwbTMsuxZabVxuCghRQc5AhWm-0VeX--e4zh14wpq4NLizM9YZiTEsB6Lvq-gPwMmhhSijiqY3QHHU-KgVoaUHu1NqCWBhQIVRoourfrB_NQIntSrZEX4N0K6GS0H0tExqUnjvO23_7jPpw5LHH8dhhVMm5pybqIJisb3H-s_AVHsasQ</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Degtyar, Pinhas</creator><creator>Neulander, Endre</creator><creator>Zirkin, Howard</creator><creator>Yusim, Igor</creator><creator>Douvdevani, Amos</creator><creator>Mermershtain, Wilmosh</creator><creator>Kaneti, Jacob</creator><creator>Manor, Ester</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040201</creationdate><title>Fluorescence in situ hybridization performed on exfoliated urothelial cells in patients with transitional cell carcinoma of the bladder</title><author>Degtyar, Pinhas ; Neulander, Endre ; Zirkin, Howard ; Yusim, Igor ; Douvdevani, Amos ; Mermershtain, Wilmosh ; Kaneti, Jacob ; Manor, Ester</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-8528528e14004be8d1d8518d00ad12186e2cbc41d5e41c4acceba898509f0dac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Aneuploidy</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Transitional Cell - diagnosis</topic><topic>Carcinoma, Transitional Cell - genetics</topic><topic>Carcinoma, Transitional Cell - pathology</topic><topic>Carcinoma, Transitional Cell - surgery</topic><topic>Carcinoma, Transitional Cell - urine</topic><topic>Chromosomes, Human, Pair 7</topic><topic>Chromosomes, Human, Pair 9</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Recurrence, Local - diagnosis</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Prognosis</topic><topic>Sensitivity and Specificity</topic><topic>Therapeutic Irrigation</topic><topic>Urinary Bladder Neoplasms - diagnosis</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary Bladder Neoplasms - pathology</topic><topic>Urinary Bladder Neoplasms - surgery</topic><topic>Urinary Bladder Neoplasms - urine</topic><topic>Urine - cytology</topic><topic>Urothelium - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Degtyar, Pinhas</creatorcontrib><creatorcontrib>Neulander, Endre</creatorcontrib><creatorcontrib>Zirkin, Howard</creatorcontrib><creatorcontrib>Yusim, Igor</creatorcontrib><creatorcontrib>Douvdevani, Amos</creatorcontrib><creatorcontrib>Mermershtain, Wilmosh</creatorcontrib><creatorcontrib>Kaneti, Jacob</creatorcontrib><creatorcontrib>Manor, Ester</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Urology (Ridgewood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Degtyar, Pinhas</au><au>Neulander, Endre</au><au>Zirkin, Howard</au><au>Yusim, Igor</au><au>Douvdevani, Amos</au><au>Mermershtain, Wilmosh</au><au>Kaneti, Jacob</au><au>Manor, Ester</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescence in situ hybridization performed on exfoliated urothelial cells in patients with transitional cell carcinoma of the bladder</atitle><jtitle>Urology (Ridgewood, N.J.)</jtitle><addtitle>Urology</addtitle><date>2004-02-01</date><risdate>2004</risdate><volume>63</volume><issue>2</issue><spage>398</spage><epage>401</epage><pages>398-401</pages><issn>0090-4295</issn><eissn>1527-9995</eissn><coden>URGYAZ</coden><abstract>To evaluate comparatively fluorescence in situ hybridization (FISH) and cytology performed on exfoliated urothelial cells obtained from voided urine and bladder washings as a method of diagnosis and follow-up in patients with transitional cell carcinoma (TCC) of the bladder.
Thirty patients with confirmed bladder TCC, 10 patients enrolled in cystoscopy follow-up for previous bladder tumors, and 10 patients with bladders free of tumor without a previous history of bladder TCC underwent cytologic examination and FISH performed on voided urine and bladder washing specimens. The FISH probes were targeted to chromosomes 7 and 9.
FISH had a sensitivity of 92% for high-grade tumors in both voided urine and bladder washing specimens, significantly greater than that of cytology at a sensitivity of 64% in voided urine and 67% in the bladder washing specimens (
P = 0.02). The sensitivity of FISH and cytology were both low and not significantly different statistically from each other for the low-grade tumors. Monosomy of chromosome 9 correlated with early tumor recurrence. Polysomy of chromosomes 7 and 9 correlated with high-grade tumors (80% and 92%, respectively).
According to our results, with the local cytopathology expertise, FISH performed on urothelial cells from voided urine has a sensitivity that supersedes that of cytology, making the former a valuable complementary method in the diagnosis and follow-up of patients with bladder TCC.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>14972510</pmid><doi>10.1016/j.urology.2003.08.026</doi><tpages>4</tpages></addata></record> |
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| ispartof | Urology (Ridgewood, N.J.), 2004-02, Vol.63 (2), p.398-401 |
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| subjects | Adult Aged Aged, 80 and over Aneuploidy Biological and medical sciences Carcinoma, Transitional Cell - diagnosis Carcinoma, Transitional Cell - genetics Carcinoma, Transitional Cell - pathology Carcinoma, Transitional Cell - surgery Carcinoma, Transitional Cell - urine Chromosomes, Human, Pair 7 Chromosomes, Human, Pair 9 Female Follow-Up Studies Humans In Situ Hybridization, Fluorescence Male Medical sciences Middle Aged Neoplasm Recurrence, Local - diagnosis Nephrology. Urinary tract diseases Prognosis Sensitivity and Specificity Therapeutic Irrigation Urinary Bladder Neoplasms - diagnosis Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - pathology Urinary Bladder Neoplasms - surgery Urinary Bladder Neoplasms - urine Urine - cytology Urothelium - ultrastructure |
| title | Fluorescence in situ hybridization performed on exfoliated urothelial cells in patients with transitional cell carcinoma of the bladder |
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