Quantitative measurement of different ceramide species from crude cellular extracts by normal-phase high-performance liquid chromatography coupled to atmospheric pressure ionization mass spectrometry

Normal‐phase high‐performance liquid chromatography (NP‐HPLC) coupled to atmospheric pressure ionization mass spectrometry (APCI‐MS) allows quantitative analysis of endogenous ceramide and dihydroceramide species from crude lipid extracts. Qualitative information for the species comes from observati...

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Veröffentlicht in:	Rapid communications in mass spectrometry 2004-01, Vol.18 (5), p.577-583
Hauptverfasser:	Pettus, Benjamin J., Kroesen, Bart-Jan, Szulc, Zdizslaw M., Bielawska, Alicia, Bielawski, Jacek, Hannun, Yusuf A., Busman, Mark
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Extracts - chemistry Ceramides - analysis Chromatography, High Pressure Liquid - methods Female HL-60 Cells - chemistry Humans Spectrometry, Mass, Electrospray Ionization - methods
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