Phosphorylation of the human cell proliferation-associated nucleolar protein P120
The human cell proliferation-associated nucleolar protein p120 was found in a variety of human cancer specimens but not in most normal resting cells. Polyclonal antibodies raised against bacterially expressed p120 were used to immunoprecipitate the p120 protein isolated from 32P-labeled HeLa cells....
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| Veröffentlicht in: | Biochemical and biophysical research communications 1990-11, Vol.173 (1), p.423-430 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Valdez, Benigno C. Busch, Rose K. Busch, Harris |
| description | The human cell proliferation-associated nucleolar protein p120 was found in a variety of human cancer specimens but not in most normal resting cells. Polyclonal antibodies raised against bacterially expressed p120 were used to immunoprecipitate the p120 protein isolated from
32P-labeled HeLa cells. The p120 protein was phosphorylated at serine, threonine and tyrosine residues. A tryptic peptide map showed it contained three labeled peptides. One of these peptides comigrated with a p120 peptide phosphorylated
in
vitro by casein kinase II. This peptide was phosphorylated
in
vitro both at Ser-181 and Thr-185. This region is juxtaposed to the epitope site recognized by the anti-p120 monoclonal antibody. |
| doi_str_mv | 10.1016/S0006-291X(05)81075-7 |
| format | Article |
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32P-labeled HeLa cells. The p120 protein was phosphorylated at serine, threonine and tyrosine residues. A tryptic peptide map showed it contained three labeled peptides. One of these peptides comigrated with a p120 peptide phosphorylated
in
vitro by casein kinase II. This peptide was phosphorylated
in
vitro both at Ser-181 and Thr-185. This region is juxtaposed to the epitope site recognized by the anti-p120 monoclonal antibody.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(05)81075-7</identifier><identifier>PMID: 2256932</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Amino Acids - analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Carrier Proteins - genetics ; Carrier Proteins - isolation & purification ; Cloning, Molecular ; Fundamental and applied biological sciences. Psychology ; HeLa Cells - metabolism ; Holoproteins ; Humans ; Maltose-Binding Proteins ; Molecular Sequence Data ; Nuclear proteins ; Nuclear Proteins - genetics ; Nuclear Proteins - immunology ; Nuclear Proteins - isolation & purification ; Nuclear Proteins - metabolism ; p120 protein ; Peptide Fragments - isolation & purification ; Phosphorylation ; Proteins ; Restriction Mapping ; tRNA Methyltransferases ; Trypsin</subject><ispartof>Biochemical and biophysical research communications, 1990-11, Vol.173 (1), p.423-430</ispartof><rights>1990 Academic Press, Inc.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-44d31d5d97175347f57660645dcd3ce2e3359b1c5e94c88aebacb6ce4be38b183</citedby><cites>FETCH-LOGICAL-c457t-44d31d5d97175347f57660645dcd3ce2e3359b1c5e94c88aebacb6ce4be38b183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X05810757$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19384853$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2256932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valdez, Benigno C.</creatorcontrib><creatorcontrib>Busch, Rose K.</creatorcontrib><creatorcontrib>Busch, Harris</creatorcontrib><title>Phosphorylation of the human cell proliferation-associated nucleolar protein P120</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The human cell proliferation-associated nucleolar protein p120 was found in a variety of human cancer specimens but not in most normal resting cells. Polyclonal antibodies raised against bacterially expressed p120 were used to immunoprecipitate the p120 protein isolated from
32P-labeled HeLa cells. The p120 protein was phosphorylated at serine, threonine and tyrosine residues. A tryptic peptide map showed it contained three labeled peptides. One of these peptides comigrated with a p120 peptide phosphorylated
in
vitro by casein kinase II. This peptide was phosphorylated
in
vitro both at Ser-181 and Thr-185. This region is juxtaposed to the epitope site recognized by the anti-p120 monoclonal antibody.</description><subject>Amino Acid Sequence</subject><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - isolation & purification</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells - metabolism</subject><subject>Holoproteins</subject><subject>Humans</subject><subject>Maltose-Binding Proteins</subject><subject>Molecular Sequence Data</subject><subject>Nuclear proteins</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - immunology</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>Nuclear Proteins - metabolism</subject><subject>p120 protein</subject><subject>Peptide Fragments - isolation & purification</subject><subject>Phosphorylation</subject><subject>Proteins</subject><subject>Restriction Mapping</subject><subject>tRNA Methyltransferases</subject><subject>Trypsin</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rFEEQhhtR4ib6EwJzUcxhtGr6c04Sgl8QMKKCt6anu4ZtmZ1eu2eE_Htndpd4zKkO7_NWFQ9jlwhvEVC9-w4Aqm5a_PUG5JVB0LLWT9gGoYW6QRBP2eYBec7OS_kNgChUe8bOmkaqljcb9u1um8p-m_L94KaYxir11bSlajvv3Fh5GoZqn9MQe8qHvHalJB_dRKEaZz9QGlxekYniWN1hAy_Ys94NhV6e5gX7-fHDj5vP9e3XT19urm9rL6SeaiECxyBDq1FLLnQvtVKghAw-cE8NcS7bDr2kVnhjHHXOd8qT6IibDg2_YK-Pe5fjf2Yqk93Fsj7sRkpzsQZQA2j1KIjSYIN8BeUR9DmVkqm3-xx3Lt9bBLs6twfndhVqQdqDc6uX3uXpwNztKDy0TpKX_NUpd8W7oc9u9LH8X95yI4zkC_f-yNGi7W-kbIuPNHoKMZOfbEjxkU_-AQKinkk</recordid><startdate>19901130</startdate><enddate>19901130</enddate><creator>Valdez, Benigno C.</creator><creator>Busch, Rose K.</creator><creator>Busch, Harris</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19901130</creationdate><title>Phosphorylation of the human cell proliferation-associated nucleolar protein P120</title><author>Valdez, Benigno C. ; Busch, Rose K. ; Busch, Harris</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-44d31d5d97175347f57660645dcd3ce2e3359b1c5e94c88aebacb6ce4be38b183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - isolation & purification</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa Cells - metabolism</topic><topic>Holoproteins</topic><topic>Humans</topic><topic>Maltose-Binding Proteins</topic><topic>Molecular Sequence Data</topic><topic>Nuclear proteins</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - immunology</topic><topic>Nuclear Proteins - isolation & purification</topic><topic>Nuclear Proteins - metabolism</topic><topic>p120 protein</topic><topic>Peptide Fragments - isolation & purification</topic><topic>Phosphorylation</topic><topic>Proteins</topic><topic>Restriction Mapping</topic><topic>tRNA Methyltransferases</topic><topic>Trypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valdez, Benigno C.</creatorcontrib><creatorcontrib>Busch, Rose K.</creatorcontrib><creatorcontrib>Busch, Harris</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valdez, Benigno C.</au><au>Busch, Rose K.</au><au>Busch, Harris</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphorylation of the human cell proliferation-associated nucleolar protein P120</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1990-11-30</date><risdate>1990</risdate><volume>173</volume><issue>1</issue><spage>423</spage><epage>430</epage><pages>423-430</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The human cell proliferation-associated nucleolar protein p120 was found in a variety of human cancer specimens but not in most normal resting cells. Polyclonal antibodies raised against bacterially expressed p120 were used to immunoprecipitate the p120 protein isolated from
32P-labeled HeLa cells. The p120 protein was phosphorylated at serine, threonine and tyrosine residues. A tryptic peptide map showed it contained three labeled peptides. One of these peptides comigrated with a p120 peptide phosphorylated
in
vitro by casein kinase II. This peptide was phosphorylated
in
vitro both at Ser-181 and Thr-185. This region is juxtaposed to the epitope site recognized by the anti-p120 monoclonal antibody.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2256932</pmid><doi>10.1016/S0006-291X(05)81075-7</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1990-11, Vol.173 (1), p.423-430 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Amino Acids - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - isolation & purification Cloning, Molecular Fundamental and applied biological sciences. Psychology HeLa Cells - metabolism Holoproteins Humans Maltose-Binding Proteins Molecular Sequence Data Nuclear proteins Nuclear Proteins - genetics Nuclear Proteins - immunology Nuclear Proteins - isolation & purification Nuclear Proteins - metabolism p120 protein Peptide Fragments - isolation & purification Phosphorylation Proteins Restriction Mapping tRNA Methyltransferases Trypsin |
| title | Phosphorylation of the human cell proliferation-associated nucleolar protein P120 |
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