Rapid simple cell suspension enzyme-linked immunosorbent assay to demonstrate and measure antibody binding

Rapid simple cell suspension enzyme-linked immunosorbent assay to demonstrate and measure antibody binding

A simple cell suspension enzyme-linked immunosorbent assay system, which can be used to test the reactivity of antibodies with cells, is described. Such a system is of particular use with cells that are difficult to fix to microtitre plates or when measuring antigens which are labile to fixation tec...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analyst (London) 1990-08, Vol.115 (8), p.1145-1146
Hauptverfasser: O'Kennedy, R J, Reading, C L
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies, Monoclonal - analysis
Antigen-Antibody Reactions
Binding, Competitive
Enzyme-Linked Immunosorbent Assay
Humans
Tumor Cells, Cultured
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1146
container_issue 8
container_start_page 1145
container_title Analyst (London)
container_volume 115
creator O'Kennedy, R J
Reading, C L
description A simple cell suspension enzyme-linked immunosorbent assay system, which can be used to test the reactivity of antibodies with cells, is described. Such a system is of particular use with cells that are difficult to fix to microtitre plates or when measuring antigens which are labile to fixation techniques.
doi_str_mv 10.1039/AN9901501145
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80165820</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80165820</sourcerecordid><originalsourceid>FETCH-LOGICAL-c310t-9710f0ffa3daa17529a21b8632097b07de70b31fe18518e4df4417935dae131e3</originalsourceid><addsrcrecordid>eNpFkEtLxDAUhYMoOj52boWsXFm9t2n6WIr4gkFBdF3Sya1kbJLa2y7GX-8MM-jqngsfB84nxDnCNYKqbm5fqgpQA2Km98QMVZ4lWqflvpgBgErSXGdH4ph5uX4RNByKwzTVudbFTCzfTO-sZOf7juSCuk7yxD0FdjFICj8rT0nnwhdZ6byfQuQ4NBRGaZjNSo5RWvIx8DiYkaQJVnoyPA2bPLom2pVsXLAufJ6Kg9Z0TGe7eyI-Hu7f756S-evj893tPFkohDGpCoQW2tYoawwWOq1Mik2ZqxSqooHCUgGNwpaw1FhSZtssw6JS2hpChaROxOW2tx_i90Q81t7xZpkJFCeuS8BclymswastuBgi80Bt3Q_Om2FVI9QbtbUJ_2rX-MWud2o82T9451L9AkREdVc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80165820</pqid></control><display><type>article</type><title>Rapid simple cell suspension enzyme-linked immunosorbent assay to demonstrate and measure antibody binding</title><source>MEDLINE</source><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>O'Kennedy, R J ; Reading, C L</creator><creatorcontrib>O'Kennedy, R J ; Reading, C L</creatorcontrib><description>A simple cell suspension enzyme-linked immunosorbent assay system, which can be used to test the reactivity of antibodies with cells, is described. Such a system is of particular use with cells that are difficult to fix to microtitre plates or when measuring antigens which are labile to fixation techniques.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/AN9901501145</identifier><identifier>PMID: 2256557</identifier><language>eng</language><publisher>England</publisher><subject>Antibodies, Monoclonal - analysis ; Antigen-Antibody Reactions ; Binding, Competitive ; Enzyme-Linked Immunosorbent Assay ; Humans ; Tumor Cells, Cultured</subject><ispartof>Analyst (London), 1990-08, Vol.115 (8), p.1145-1146</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c310t-9710f0ffa3daa17529a21b8632097b07de70b31fe18518e4df4417935dae131e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2256557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Kennedy, R J</creatorcontrib><creatorcontrib>Reading, C L</creatorcontrib><title>Rapid simple cell suspension enzyme-linked immunosorbent assay to demonstrate and measure antibody binding</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>A simple cell suspension enzyme-linked immunosorbent assay system, which can be used to test the reactivity of antibodies with cells, is described. Such a system is of particular use with cells that are difficult to fix to microtitre plates or when measuring antigens which are labile to fixation techniques.</description><subject>Antibodies, Monoclonal - analysis</subject><subject>Antigen-Antibody Reactions</subject><subject>Binding, Competitive</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>Tumor Cells, Cultured</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLxDAUhYMoOj52boWsXFm9t2n6WIr4gkFBdF3Sya1kbJLa2y7GX-8MM-jqngsfB84nxDnCNYKqbm5fqgpQA2Km98QMVZ4lWqflvpgBgErSXGdH4ph5uX4RNByKwzTVudbFTCzfTO-sZOf7juSCuk7yxD0FdjFICj8rT0nnwhdZ6byfQuQ4NBRGaZjNSo5RWvIx8DiYkaQJVnoyPA2bPLom2pVsXLAufJ6Kg9Z0TGe7eyI-Hu7f756S-evj893tPFkohDGpCoQW2tYoawwWOq1Mik2ZqxSqooHCUgGNwpaw1FhSZtssw6JS2hpChaROxOW2tx_i90Q81t7xZpkJFCeuS8BclymswastuBgi80Bt3Q_Om2FVI9QbtbUJ_2rX-MWud2o82T9451L9AkREdVc</recordid><startdate>19900801</startdate><enddate>19900801</enddate><creator>O'Kennedy, R J</creator><creator>Reading, C L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900801</creationdate><title>Rapid simple cell suspension enzyme-linked immunosorbent assay to demonstrate and measure antibody binding</title><author>O'Kennedy, R J ; Reading, C L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c310t-9710f0ffa3daa17529a21b8632097b07de70b31fe18518e4df4417935dae131e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Antibodies, Monoclonal - analysis</topic><topic>Antigen-Antibody Reactions</topic><topic>Binding, Competitive</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Humans</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Kennedy, R J</creatorcontrib><creatorcontrib>Reading, C L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Kennedy, R J</au><au>Reading, C L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid simple cell suspension enzyme-linked immunosorbent assay to demonstrate and measure antibody binding</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>1990-08-01</date><risdate>1990</risdate><volume>115</volume><issue>8</issue><spage>1145</spage><epage>1146</epage><pages>1145-1146</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>A simple cell suspension enzyme-linked immunosorbent assay system, which can be used to test the reactivity of antibodies with cells, is described. Such a system is of particular use with cells that are difficult to fix to microtitre plates or when measuring antigens which are labile to fixation techniques.</abstract><cop>England</cop><pmid>2256557</pmid><doi>10.1039/AN9901501145</doi><tpages>2</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0003-2654
ispartof Analyst (London), 1990-08, Vol.115 (8), p.1145-1146
issn 0003-2654
1364-5528
language eng
recordid cdi_proquest_miscellaneous_80165820
source MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Antibodies, Monoclonal - analysis
Antigen-Antibody Reactions
Binding, Competitive
Enzyme-Linked Immunosorbent Assay
Humans
Tumor Cells, Cultured
title Rapid simple cell suspension enzyme-linked immunosorbent assay to demonstrate and measure antibody binding
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T14%3A16%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20simple%20cell%20suspension%20enzyme-linked%20immunosorbent%20assay%20to%20demonstrate%20and%20measure%20antibody%20binding&rft.jtitle=Analyst%20(London)&rft.au=O'Kennedy,%20R%20J&rft.date=1990-08-01&rft.volume=115&rft.issue=8&rft.spage=1145&rft.epage=1146&rft.pages=1145-1146&rft.issn=0003-2654&rft.eissn=1364-5528&rft_id=info:doi/10.1039/AN9901501145&rft_dat=%3Cproquest_cross%3E80165820%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80165820&rft_id=info:pmid/2256557&rfr_iscdi=true