Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy
Quantitative evaluation of the dose enhancement obtained with analog nucleoside agents such as iododeoxyuridme (IdUrd) requires knowledge of the degree to which the thymidine (Thd) in DNA is replaced by IdUrd. In the present investigation, mice were infused with IdUrd using an intravenous infusion a...
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| Veröffentlicht in: | Radiotherapy and oncology 1990-10, Vol.19 (2), p.169-178 |
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| container_title | Radiotherapy and oncology |
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| creator | Laster, B.H. Popenoe, E.A. Wielopolski, L. Commerford, S.L. Gahbauer, R. Goodman, J. Meek, A. Fairchild, R.G. |
| description | Quantitative evaluation of the dose enhancement obtained with analog nucleoside agents such as iododeoxyuridme (IdUrd) requires knowledge of the degree to which the thymidine (Thd) in DNA is replaced by IdUrd. In the present investigation, mice were infused with IdUrd using an intravenous infusion apparatus capable of delivering continuous multi-day infusions without restraining the mice. The absolute incorporation of IdUrd in DNA was measured by
125IdUrd label, both in whole tissue and extracted DNA, showing a good correlation between levels observed in DNA and whole tissue. Replacement in a Harding-Passey murine melanoma tumor carried in BALB/c mice approached 10%. In addition, a Neutron Activation Analysis (NAA) technique was developed which showed in vitro, a sensitivity sufficient to evaluate the ‰ replacement of Thd by IdUrd in small biological samples with a sensitivity greater than 0.1 ppm, at 1 ‰ replacement in mg samples. This method can provide information on iodine substitution in DNA in humans where the. use of a radioactive DNA-seeking substance would be undesirable. Analyses of IdUrd incorporation in cultured cells by NAA and
125I counting showed good agreement. |
| doi_str_mv | 10.1016/0167-8140(90)90130-O |
| format | Article |
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125IdUrd label, both in whole tissue and extracted DNA, showing a good correlation between levels observed in DNA and whole tissue. Replacement in a Harding-Passey murine melanoma tumor carried in BALB/c mice approached 10%. In addition, a Neutron Activation Analysis (NAA) technique was developed which showed in vitro, a sensitivity sufficient to evaluate the ‰ replacement of Thd by IdUrd in small biological samples with a sensitivity greater than 0.1 ppm, at 1 ‰ replacement in mg samples. This method can provide information on iodine substitution in DNA in humans where the. use of a radioactive DNA-seeking substance would be undesirable. Analyses of IdUrd incorporation in cultured cells by NAA and
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125IdUrd label, both in whole tissue and extracted DNA, showing a good correlation between levels observed in DNA and whole tissue. Replacement in a Harding-Passey murine melanoma tumor carried in BALB/c mice approached 10%. In addition, a Neutron Activation Analysis (NAA) technique was developed which showed in vitro, a sensitivity sufficient to evaluate the ‰ replacement of Thd by IdUrd in small biological samples with a sensitivity greater than 0.1 ppm, at 1 ‰ replacement in mg samples. This method can provide information on iodine substitution in DNA in humans where the. use of a radioactive DNA-seeking substance would be undesirable. Analyses of IdUrd incorporation in cultured cells by NAA and
125I counting showed good agreement.</description><subject>127IdUrd</subject><subject>Animals</subject><subject>Continuous infusion</subject><subject>DNA, Neoplasm - metabolism</subject><subject>Idoxuridine - administration & dosage</subject><subject>Idoxuridine - therapeutic use</subject><subject>Infusion Pumps</subject><subject>Iodine Radioisotopes</subject><subject>Melanoma, Experimental - radiotherapy</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neutron Activation Analysis</subject><subject>Nucleoside analog substitution</subject><subject>Photon activation therapy</subject><subject>Radiation-Sensitizing Agents</subject><subject>Thymidine - metabolism</subject><issn>0167-8140</issn><issn>1879-0887</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtKBDEQhoMoOj5uoNAr0UU0j-5JshFEfIEwG12HdFLByHSnTXrE2Xkmj-RJzDCDS6FCkfr_qlQ-hI4puaCETi_LEVjSmpwpcq4I5QTPttCESqEwkVJso8mfZQ_t5_xGCGGEi120y1jTCEEmyF33Zr7MIVfRVw0O0UXMfr6-K-wgfi4XKbjQQxV6G9MQkxlD7Mut6opS6h3MTR87U_mYquE1jkU1dgwfa-P4CskMy0O04808w9EmH6CXu9vnmwf8NLt_vLl-wpY3YsSOA7OeUeEMGDGtKTetIpJ67qx3U6moamsPjW2kYC1IyRXjviVy2kopleEH6HQ9d0jxfQF51F3IFuZlR4iLrGXhwWpSF2O9NtoUc07g9ZBCZ9JSU6JXcPWKnF6R02oVBa6elbaTzfxF24H7a9rQLPrVWofyyY8ASWcboLfgQgI7ahfD_w_8AoSpiyM</recordid><startdate>19901001</startdate><enddate>19901001</enddate><creator>Laster, B.H.</creator><creator>Popenoe, E.A.</creator><creator>Wielopolski, L.</creator><creator>Commerford, S.L.</creator><creator>Gahbauer, R.</creator><creator>Goodman, J.</creator><creator>Meek, A.</creator><creator>Fairchild, R.G.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19901001</creationdate><title>Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy</title><author>Laster, B.H. ; Popenoe, E.A. ; Wielopolski, L. ; Commerford, S.L. ; Gahbauer, R. ; Goodman, J. ; Meek, A. ; Fairchild, R.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-d3e2cf217daea76413ab9081f3dcfd68919b4fe5c5872be883923fb086b8889a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>127IdUrd</topic><topic>Animals</topic><topic>Continuous infusion</topic><topic>DNA, Neoplasm - metabolism</topic><topic>Idoxuridine - administration & dosage</topic><topic>Idoxuridine - therapeutic use</topic><topic>Infusion Pumps</topic><topic>Iodine Radioisotopes</topic><topic>Melanoma, Experimental - radiotherapy</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neutron Activation Analysis</topic><topic>Nucleoside analog substitution</topic><topic>Photon activation therapy</topic><topic>Radiation-Sensitizing Agents</topic><topic>Thymidine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laster, B.H.</creatorcontrib><creatorcontrib>Popenoe, E.A.</creatorcontrib><creatorcontrib>Wielopolski, L.</creatorcontrib><creatorcontrib>Commerford, S.L.</creatorcontrib><creatorcontrib>Gahbauer, R.</creatorcontrib><creatorcontrib>Goodman, J.</creatorcontrib><creatorcontrib>Meek, A.</creatorcontrib><creatorcontrib>Fairchild, R.G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Radiotherapy and oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laster, B.H.</au><au>Popenoe, E.A.</au><au>Wielopolski, L.</au><au>Commerford, S.L.</au><au>Gahbauer, R.</au><au>Goodman, J.</au><au>Meek, A.</au><au>Fairchild, R.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy</atitle><jtitle>Radiotherapy and oncology</jtitle><addtitle>Radiother Oncol</addtitle><date>1990-10-01</date><risdate>1990</risdate><volume>19</volume><issue>2</issue><spage>169</spage><epage>178</epage><pages>169-178</pages><issn>0167-8140</issn><eissn>1879-0887</eissn><abstract>Quantitative evaluation of the dose enhancement obtained with analog nucleoside agents such as iododeoxyuridme (IdUrd) requires knowledge of the degree to which the thymidine (Thd) in DNA is replaced by IdUrd. In the present investigation, mice were infused with IdUrd using an intravenous infusion apparatus capable of delivering continuous multi-day infusions without restraining the mice. The absolute incorporation of IdUrd in DNA was measured by
125IdUrd label, both in whole tissue and extracted DNA, showing a good correlation between levels observed in DNA and whole tissue. Replacement in a Harding-Passey murine melanoma tumor carried in BALB/c mice approached 10%. In addition, a Neutron Activation Analysis (NAA) technique was developed which showed in vitro, a sensitivity sufficient to evaluate the ‰ replacement of Thd by IdUrd in small biological samples with a sensitivity greater than 0.1 ppm, at 1 ‰ replacement in mg samples. This method can provide information on iodine substitution in DNA in humans where the. use of a radioactive DNA-seeking substance would be undesirable. Analyses of IdUrd incorporation in cultured cells by NAA and
125I counting showed good agreement.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>2255770</pmid><doi>10.1016/0167-8140(90)90130-O</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 127IdUrd Animals Continuous infusion DNA, Neoplasm - metabolism Idoxuridine - administration & dosage Idoxuridine - therapeutic use Infusion Pumps Iodine Radioisotopes Melanoma, Experimental - radiotherapy Mice Mice, Inbred BALB C Neutron Activation Analysis Nucleoside analog substitution Photon activation therapy Radiation-Sensitizing Agents Thymidine - metabolism |
| title | Analysis of 5-iodo-2′ -deoxyuridine incorporation in murine melanoma for photon activation therapy |
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