HP1 Is Essential for DNA Methylation in Neurospora
Methylation of cytosines silences transposable elements and selected cellular genes in mammals, plants, and some fungi. Recent findings have revealed mechanistic connections between DNA methylation and features of specialized condensed chromatin, “heterochromatin.” In Neurospora crassa, DNA methylat...
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| Veröffentlicht in: | Molecular cell 2004-02, Vol.13 (3), p.427-434 |
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| creator | Freitag, Michael Hickey, Patrick C. Khlafallah, Tamir K. Read, Nick D. Selker, Eric U. |
| description | Methylation of cytosines silences transposable elements and selected cellular genes in mammals, plants, and some fungi. Recent findings have revealed mechanistic connections between DNA methylation and features of specialized condensed chromatin, “heterochromatin.” In
Neurospora crassa, DNA methylation depends on trimethylation of Lys9 in histone H3 by DIM-5. Heterochromatin protein HP1 binds methylated Lys9 in vitro. We therefore investigated the possibility that a
Neurospora HP1 homolog reads the methyl-Lys9 mark to signal DNA methylation. We identified an HP1 homolog and showed that it is essential for DNA methylation, is localized to heterochromatic foci, and that this localization is dependent on the catalytic activity of DIM-5. We conclude that HP1 serves as an adaptor between methylated H3 Lys9 and the DNA methylation machinery. Unlike mutants that lack DNA methyltransferase, mutants with defects in the HP1 gene
hpo exhibit severe growth defects, suggesting that HP1 is required for processes besides DNA methylation. |
| doi_str_mv | 10.1016/S1097-2765(04)00024-3 |
| format | Article |
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Neurospora crassa, DNA methylation depends on trimethylation of Lys9 in histone H3 by DIM-5. Heterochromatin protein HP1 binds methylated Lys9 in vitro. We therefore investigated the possibility that a
Neurospora HP1 homolog reads the methyl-Lys9 mark to signal DNA methylation. We identified an HP1 homolog and showed that it is essential for DNA methylation, is localized to heterochromatic foci, and that this localization is dependent on the catalytic activity of DIM-5. We conclude that HP1 serves as an adaptor between methylated H3 Lys9 and the DNA methylation machinery. Unlike mutants that lack DNA methyltransferase, mutants with defects in the HP1 gene
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Neurospora crassa, DNA methylation depends on trimethylation of Lys9 in histone H3 by DIM-5. Heterochromatin protein HP1 binds methylated Lys9 in vitro. We therefore investigated the possibility that a
Neurospora HP1 homolog reads the methyl-Lys9 mark to signal DNA methylation. We identified an HP1 homolog and showed that it is essential for DNA methylation, is localized to heterochromatic foci, and that this localization is dependent on the catalytic activity of DIM-5. We conclude that HP1 serves as an adaptor between methylated H3 Lys9 and the DNA methylation machinery. Unlike mutants that lack DNA methyltransferase, mutants with defects in the HP1 gene
hpo exhibit severe growth defects, suggesting that HP1 is required for processes besides DNA methylation.</description><subject>AHP1 protein</subject><subject>Catalytic Domain - genetics</subject><subject>Chromosomal Proteins, Non-Histone - genetics</subject><subject>Chromosomal Proteins, Non-Histone - isolation & purification</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>DNA - metabolism</subject><subject>DNA Methylation</subject><subject>DNA, Complementary - analysis</subject><subject>DNA, Complementary - genetics</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - isolation & purification</subject><subject>Fungal Proteins - metabolism</subject><subject>Heterochromatin - genetics</subject><subject>Histone-Lysine N-Methyltransferase</subject><subject>HP1 protein</subject><subject>hpo gene</subject><subject>Lysine - metabolism</subject><subject>Methyltransferases - genetics</subject><subject>Molecular Sequence Data</subject><subject>Neurospora</subject><subject>Neurospora crassa - enzymology</subject><subject>Neurospora crassa - genetics</subject><subject>Protein Methyltransferases</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>1097-2765</issn><issn>1097-4164</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFFLwzAQgIMobk5_gtIn0YdqkiZp8iRjTjeYU1CfQ9pcMdK1M2mF_Xu7reDjXu6O47s77kPokuA7gom4fydYpTFNBb_B7BZjTFmcHKHhrs2IYMd9vUUG6CyEb4wJ41KdogFhSqRdGCI6eyPRPETTEKBqnCmjovbR43IcvUDztSlN4-oqclW0hNbXYV17c45OClMGuOjzCH0-TT8ms3jx-jyfjBdxzhLVxEXKRVZIllJhBRCprM1ykVomuUy4EZkAbC2WwIAyw7qySGiBlTEpxTxhyQhd7_euff3TQmj0yoUcytJUULdBy04DpVwdBImighEqO5Dvwbx7JXgo9Nq7lfEbTbDeWtU7q3qrTGOmd1Z10s1d9QfabAX2f6rX2AEPewA6H78OvA65gyoH6zzkjba1O3DiD05uhGY</recordid><startdate>20040213</startdate><enddate>20040213</enddate><creator>Freitag, Michael</creator><creator>Hickey, Patrick C.</creator><creator>Khlafallah, Tamir K.</creator><creator>Read, Nick D.</creator><creator>Selker, Eric U.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20040213</creationdate><title>HP1 Is Essential for DNA Methylation in Neurospora</title><author>Freitag, Michael ; Hickey, Patrick C. ; Khlafallah, Tamir K. ; Read, Nick D. ; Selker, Eric U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-f756bf84726d6e189ddbc67d485835a6b6e0dd08e4e24a4dd0f32f09aa7205343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>AHP1 protein</topic><topic>Catalytic Domain - genetics</topic><topic>Chromosomal Proteins, Non-Histone - genetics</topic><topic>Chromosomal Proteins, Non-Histone - isolation & purification</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>DNA - metabolism</topic><topic>DNA Methylation</topic><topic>DNA, Complementary - analysis</topic><topic>DNA, Complementary - genetics</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - isolation & purification</topic><topic>Fungal Proteins - metabolism</topic><topic>Heterochromatin - genetics</topic><topic>Histone-Lysine N-Methyltransferase</topic><topic>HP1 protein</topic><topic>hpo gene</topic><topic>Lysine - metabolism</topic><topic>Methyltransferases - genetics</topic><topic>Molecular Sequence Data</topic><topic>Neurospora</topic><topic>Neurospora crassa - enzymology</topic><topic>Neurospora crassa - genetics</topic><topic>Protein Methyltransferases</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freitag, Michael</creatorcontrib><creatorcontrib>Hickey, Patrick C.</creatorcontrib><creatorcontrib>Khlafallah, Tamir K.</creatorcontrib><creatorcontrib>Read, Nick D.</creatorcontrib><creatorcontrib>Selker, Eric U.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freitag, Michael</au><au>Hickey, Patrick C.</au><au>Khlafallah, Tamir K.</au><au>Read, Nick D.</au><au>Selker, Eric U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HP1 Is Essential for DNA Methylation in Neurospora</atitle><jtitle>Molecular cell</jtitle><addtitle>Mol Cell</addtitle><date>2004-02-13</date><risdate>2004</risdate><volume>13</volume><issue>3</issue><spage>427</spage><epage>434</epage><pages>427-434</pages><issn>1097-2765</issn><eissn>1097-4164</eissn><abstract>Methylation of cytosines silences transposable elements and selected cellular genes in mammals, plants, and some fungi. Recent findings have revealed mechanistic connections between DNA methylation and features of specialized condensed chromatin, “heterochromatin.” In
Neurospora crassa, DNA methylation depends on trimethylation of Lys9 in histone H3 by DIM-5. Heterochromatin protein HP1 binds methylated Lys9 in vitro. We therefore investigated the possibility that a
Neurospora HP1 homolog reads the methyl-Lys9 mark to signal DNA methylation. We identified an HP1 homolog and showed that it is essential for DNA methylation, is localized to heterochromatic foci, and that this localization is dependent on the catalytic activity of DIM-5. We conclude that HP1 serves as an adaptor between methylated H3 Lys9 and the DNA methylation machinery. Unlike mutants that lack DNA methyltransferase, mutants with defects in the HP1 gene
hpo exhibit severe growth defects, suggesting that HP1 is required for processes besides DNA methylation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>14967149</pmid><doi>10.1016/S1097-2765(04)00024-3</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | AHP1 protein Catalytic Domain - genetics Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - isolation & purification Chromosomal Proteins, Non-Histone - metabolism DNA - metabolism DNA Methylation DNA, Complementary - analysis DNA, Complementary - genetics Fungal Proteins - genetics Fungal Proteins - isolation & purification Fungal Proteins - metabolism Heterochromatin - genetics Histone-Lysine N-Methyltransferase HP1 protein hpo gene Lysine - metabolism Methyltransferases - genetics Molecular Sequence Data Neurospora Neurospora crassa - enzymology Neurospora crassa - genetics Protein Methyltransferases Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid |
| title | HP1 Is Essential for DNA Methylation in Neurospora |
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