Envelope membranes from mature spinach chloroplasts contain a NADPH:protochlorophyllide reductase on the cytosolic side of the outer membrane

Using fluorescence spectroscopy, we have demonstrated that isolated envelope membranes from mature spinach chloroplasts catalyze the phototransformation of endogenous protochlorophyllide into chlorophyllide in presence of NADPH, but not in presence of NADH. Protochlorophyllide reductase was characte...

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Veröffentlicht in:	The Journal of biological chemistry 1990-12, Vol.265 (35), p.21820-21827
Hauptverfasser:	Joyard, J, Block, M, Pineau, B, Albrieux, C, Douce, R
Format:	Artikel
Sprache:	eng
Schlagworte:	CELL MEMBRANES CHARACTERIZATION CHLOROPHYLLE CHLOROPHYLLS CHLOROPLASTE CHLOROPLASTS Chloroplasts - enzymology Chloroplasts - ultrastructure CLOROFILAS CLOROPLASTO COENZIMAS COENZYME COENZYMES envelopes Intracellular Membranes - enzymology Light MEMBRANAS CELULARES MEMBRANE CELLULAIRE Molecular Weight NADP - physiology NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS outer membranes OXIDOREDUCTASES Oxidoreductases - immunology Oxidoreductases - metabolism Oxidoreductases Acting on CH-CH Group Donors OXIDORREDUCTASAS OXYDOREDUCTASE Plant Proteins - chemistry Plant Proteins - immunology Plants protochlorophyllide reductase Spectrometry, Fluorescence SPINACIA OLERACEA
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