Mechanical modulation of cartilage structure and function during embryogenesis in the chick
The mechanical behavior of cartilage is intimately related to its biochemical composition, and tissue composition is known to be influenced by its local mechanical loading environment. Although this phenomenon has been well-studied in adult cartilage, few investigations have examined such structure-...
Gespeichert in:
| Veröffentlicht in: | Annals of biomedical engineering 2004-01, Vol.32 (1), p.18-25 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 25 |
|---|---|
| container_issue | 1 |
| container_start_page | 18 |
| container_title | Annals of biomedical engineering |
| container_volume | 32 |
| creator | Mikic, Borjana Isenstein, Arin Lynn Chhabra, Abhinav |
| description | The mechanical behavior of cartilage is intimately related to its biochemical composition, and tissue composition is known to be influenced by its local mechanical loading environment. Although this phenomenon has been well-studied in adult cartilage, few investigations have examined such structure-function relationships in embryonic cartilage. The goal of this work was to elucidate the role of mechanical loading on the development of cartilage composition during embryogenesis. Using an embryonic chick model, cartilage from the tibiofemoral joints of immobilized embryos was compared to that of controls. The normal time course of changes in glycosaminoglycan/DNA and hydroxyproline/DNA were significantly influenced by loading history, with the most pronounced effects observed between days 9 and 14 during the period of most rapid increase in motility in control embryos. Stress-relaxation tests conducted on samples from day 14 indicate that the effects of embryonic immobilization on cartilage matrix composition have direct consequences for the mechanical behavior of the tissue, resulting in compromised material properties (e.g. 50% reduction in E(inst)). Because embryogenesis provides a unique model for identifying key factors which influence the establishment of functional biomechanical tissues in the skeleton, these data suggest that treating mechanical loading as an in vitro culture variable for tissue engineering approaches to cartilage repair is likely to be a sound approach. |
| doi_str_mv | 10.1023/b:abme.0000007787.39262.a7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80159236</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1875848491</sourcerecordid><originalsourceid>FETCH-LOGICAL-c472t-f62e1ccfef660477e1f999ee514e3e9a1743b3b47895ca4396a0c65c5ad9428d3</originalsourceid><addsrcrecordid>eNqFkT1rHDEQhkVIiC92_oIRLoKbvej7w53POB9gkyauUgitdvZO9q7WkVaF_7035wNDimSageF5ZxgehM4oWVPC-Of2wrcjrMm-tDZ6zS1TbO31G7SiUvPGKqPeohUhljTKKnGEPpRyTwilhsv36IiKZaipWaFftxB2PsXgBzxOXR38HKeEpx4Hn-c4-C3gMuca5poB-9ThvqawZ7qaY9piGNv8NG0hQYkFx4TnHeCwi-HhBL3r_VDg46Efo7sv1z-vvjU3P75-v7q8aYLQbG56xYCG0EOvFBFaA-2ttQCSCuBgPdWCt7wV2lgZvOBWeRKUDNJ3VjDT8WP06WXvY55-VyizG2MJMAw-wVSLM4RKy7hawPN_gtRoaYQRlv53J9XKaCbFAp79Bd5PNaflX2ctM4oSzRbo4gUKeSolQ-8ecxx9fnKUuD9O3cZdbm6v3atTt3fqvF7Cp4cLddHevUYPEvkznByecQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>992861072</pqid></control><display><type>article</type><title>Mechanical modulation of cartilage structure and function during embryogenesis in the chick</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Mikic, Borjana ; Isenstein, Arin Lynn ; Chhabra, Abhinav</creator><creatorcontrib>Mikic, Borjana ; Isenstein, Arin Lynn ; Chhabra, Abhinav</creatorcontrib><description>The mechanical behavior of cartilage is intimately related to its biochemical composition, and tissue composition is known to be influenced by its local mechanical loading environment. Although this phenomenon has been well-studied in adult cartilage, few investigations have examined such structure-function relationships in embryonic cartilage. The goal of this work was to elucidate the role of mechanical loading on the development of cartilage composition during embryogenesis. Using an embryonic chick model, cartilage from the tibiofemoral joints of immobilized embryos was compared to that of controls. The normal time course of changes in glycosaminoglycan/DNA and hydroxyproline/DNA were significantly influenced by loading history, with the most pronounced effects observed between days 9 and 14 during the period of most rapid increase in motility in control embryos. Stress-relaxation tests conducted on samples from day 14 indicate that the effects of embryonic immobilization on cartilage matrix composition have direct consequences for the mechanical behavior of the tissue, resulting in compromised material properties (e.g. 50% reduction in E(inst)). Because embryogenesis provides a unique model for identifying key factors which influence the establishment of functional biomechanical tissues in the skeleton, these data suggest that treating mechanical loading as an in vitro culture variable for tissue engineering approaches to cartilage repair is likely to be a sound approach.</description><identifier>ISSN: 0090-6964</identifier><identifier>EISSN: 1573-9686</identifier><identifier>DOI: 10.1023/b:abme.0000007787.39262.a7</identifier><identifier>PMID: 14964718</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Adaptation, Physiological - physiology ; Animals ; Biochemical composition ; Cartilage, Articular - cytology ; Cartilage, Articular - drug effects ; Cartilage, Articular - embryology ; Cartilage, Articular - physiology ; Chick Embryo ; Chickens ; Collagen - metabolism ; Decamethonium Compounds - pharmacology ; Deoxyribonucleic acid ; DNA ; Elasticity ; Embryonic growth stage ; Embryos ; Glycosaminoglycans - metabolism ; Hydroxyproline - metabolism ; Immobilization ; Knee Joint - drug effects ; Knee Joint - growth & development ; Knee Joint - physiology ; Mechanotransduction, Cellular - drug effects ; Mechanotransduction, Cellular - physiology ; Stress, Mechanical ; Weight-Bearing - physiology</subject><ispartof>Annals of biomedical engineering, 2004-01, Vol.32 (1), p.18-25</ispartof><rights>Biomedical Engineering Society 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-f62e1ccfef660477e1f999ee514e3e9a1743b3b47895ca4396a0c65c5ad9428d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14964718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mikic, Borjana</creatorcontrib><creatorcontrib>Isenstein, Arin Lynn</creatorcontrib><creatorcontrib>Chhabra, Abhinav</creatorcontrib><title>Mechanical modulation of cartilage structure and function during embryogenesis in the chick</title><title>Annals of biomedical engineering</title><addtitle>Ann Biomed Eng</addtitle><description>The mechanical behavior of cartilage is intimately related to its biochemical composition, and tissue composition is known to be influenced by its local mechanical loading environment. Although this phenomenon has been well-studied in adult cartilage, few investigations have examined such structure-function relationships in embryonic cartilage. The goal of this work was to elucidate the role of mechanical loading on the development of cartilage composition during embryogenesis. Using an embryonic chick model, cartilage from the tibiofemoral joints of immobilized embryos was compared to that of controls. The normal time course of changes in glycosaminoglycan/DNA and hydroxyproline/DNA were significantly influenced by loading history, with the most pronounced effects observed between days 9 and 14 during the period of most rapid increase in motility in control embryos. Stress-relaxation tests conducted on samples from day 14 indicate that the effects of embryonic immobilization on cartilage matrix composition have direct consequences for the mechanical behavior of the tissue, resulting in compromised material properties (e.g. 50% reduction in E(inst)). Because embryogenesis provides a unique model for identifying key factors which influence the establishment of functional biomechanical tissues in the skeleton, these data suggest that treating mechanical loading as an in vitro culture variable for tissue engineering approaches to cartilage repair is likely to be a sound approach.</description><subject>Adaptation, Physiological - physiology</subject><subject>Animals</subject><subject>Biochemical composition</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - drug effects</subject><subject>Cartilage, Articular - embryology</subject><subject>Cartilage, Articular - physiology</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Collagen - metabolism</subject><subject>Decamethonium Compounds - pharmacology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Elasticity</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Glycosaminoglycans - metabolism</subject><subject>Hydroxyproline - metabolism</subject><subject>Immobilization</subject><subject>Knee Joint - drug effects</subject><subject>Knee Joint - growth & development</subject><subject>Knee Joint - physiology</subject><subject>Mechanotransduction, Cellular - drug effects</subject><subject>Mechanotransduction, Cellular - physiology</subject><subject>Stress, Mechanical</subject><subject>Weight-Bearing - physiology</subject><issn>0090-6964</issn><issn>1573-9686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkT1rHDEQhkVIiC92_oIRLoKbvej7w53POB9gkyauUgitdvZO9q7WkVaF_7035wNDimSageF5ZxgehM4oWVPC-Of2wrcjrMm-tDZ6zS1TbO31G7SiUvPGKqPeohUhljTKKnGEPpRyTwilhsv36IiKZaipWaFftxB2PsXgBzxOXR38HKeEpx4Hn-c4-C3gMuca5poB-9ThvqawZ7qaY9piGNv8NG0hQYkFx4TnHeCwi-HhBL3r_VDg46Efo7sv1z-vvjU3P75-v7q8aYLQbG56xYCG0EOvFBFaA-2ttQCSCuBgPdWCt7wV2lgZvOBWeRKUDNJ3VjDT8WP06WXvY55-VyizG2MJMAw-wVSLM4RKy7hawPN_gtRoaYQRlv53J9XKaCbFAp79Bd5PNaflX2ctM4oSzRbo4gUKeSolQ-8ecxx9fnKUuD9O3cZdbm6v3atTt3fqvF7Cp4cLddHevUYPEvkznByecQ</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Mikic, Borjana</creator><creator>Isenstein, Arin Lynn</creator><creator>Chhabra, Abhinav</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7X8</scope></search><sort><creationdate>200401</creationdate><title>Mechanical modulation of cartilage structure and function during embryogenesis in the chick</title><author>Mikic, Borjana ; Isenstein, Arin Lynn ; Chhabra, Abhinav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-f62e1ccfef660477e1f999ee514e3e9a1743b3b47895ca4396a0c65c5ad9428d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adaptation, Physiological - physiology</topic><topic>Animals</topic><topic>Biochemical composition</topic><topic>Cartilage, Articular - cytology</topic><topic>Cartilage, Articular - drug effects</topic><topic>Cartilage, Articular - embryology</topic><topic>Cartilage, Articular - physiology</topic><topic>Chick Embryo</topic><topic>Chickens</topic><topic>Collagen - metabolism</topic><topic>Decamethonium Compounds - pharmacology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Elasticity</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Glycosaminoglycans - metabolism</topic><topic>Hydroxyproline - metabolism</topic><topic>Immobilization</topic><topic>Knee Joint - drug effects</topic><topic>Knee Joint - growth & development</topic><topic>Knee Joint - physiology</topic><topic>Mechanotransduction, Cellular - drug effects</topic><topic>Mechanotransduction, Cellular - physiology</topic><topic>Stress, Mechanical</topic><topic>Weight-Bearing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mikic, Borjana</creatorcontrib><creatorcontrib>Isenstein, Arin Lynn</creatorcontrib><creatorcontrib>Chhabra, Abhinav</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of biomedical engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mikic, Borjana</au><au>Isenstein, Arin Lynn</au><au>Chhabra, Abhinav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanical modulation of cartilage structure and function during embryogenesis in the chick</atitle><jtitle>Annals of biomedical engineering</jtitle><addtitle>Ann Biomed Eng</addtitle><date>2004-01</date><risdate>2004</risdate><volume>32</volume><issue>1</issue><spage>18</spage><epage>25</epage><pages>18-25</pages><issn>0090-6964</issn><eissn>1573-9686</eissn><abstract>The mechanical behavior of cartilage is intimately related to its biochemical composition, and tissue composition is known to be influenced by its local mechanical loading environment. Although this phenomenon has been well-studied in adult cartilage, few investigations have examined such structure-function relationships in embryonic cartilage. The goal of this work was to elucidate the role of mechanical loading on the development of cartilage composition during embryogenesis. Using an embryonic chick model, cartilage from the tibiofemoral joints of immobilized embryos was compared to that of controls. The normal time course of changes in glycosaminoglycan/DNA and hydroxyproline/DNA were significantly influenced by loading history, with the most pronounced effects observed between days 9 and 14 during the period of most rapid increase in motility in control embryos. Stress-relaxation tests conducted on samples from day 14 indicate that the effects of embryonic immobilization on cartilage matrix composition have direct consequences for the mechanical behavior of the tissue, resulting in compromised material properties (e.g. 50% reduction in E(inst)). Because embryogenesis provides a unique model for identifying key factors which influence the establishment of functional biomechanical tissues in the skeleton, these data suggest that treating mechanical loading as an in vitro culture variable for tissue engineering approaches to cartilage repair is likely to be a sound approach.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>14964718</pmid><doi>10.1023/b:abme.0000007787.39262.a7</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0090-6964 |
| ispartof | Annals of biomedical engineering, 2004-01, Vol.32 (1), p.18-25 |
| issn | 0090-6964 1573-9686 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80159236 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adaptation, Physiological - physiology Animals Biochemical composition Cartilage, Articular - cytology Cartilage, Articular - drug effects Cartilage, Articular - embryology Cartilage, Articular - physiology Chick Embryo Chickens Collagen - metabolism Decamethonium Compounds - pharmacology Deoxyribonucleic acid DNA Elasticity Embryonic growth stage Embryos Glycosaminoglycans - metabolism Hydroxyproline - metabolism Immobilization Knee Joint - drug effects Knee Joint - growth & development Knee Joint - physiology Mechanotransduction, Cellular - drug effects Mechanotransduction, Cellular - physiology Stress, Mechanical Weight-Bearing - physiology |
| title | Mechanical modulation of cartilage structure and function during embryogenesis in the chick |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T18%3A37%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mechanical%20modulation%20of%20cartilage%20structure%20and%20function%20during%20embryogenesis%20in%20the%20chick&rft.jtitle=Annals%20of%20biomedical%20engineering&rft.au=Mikic,%20Borjana&rft.date=2004-01&rft.volume=32&rft.issue=1&rft.spage=18&rft.epage=25&rft.pages=18-25&rft.issn=0090-6964&rft.eissn=1573-9686&rft_id=info:doi/10.1023/b:abme.0000007787.39262.a7&rft_dat=%3Cproquest_cross%3E1875848491%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=992861072&rft_id=info:pmid/14964718&rfr_iscdi=true |