In vitro polyadenylation is stimulated by the presence of an upstream intron
The majority of vertebrate pre-mRNAs are both spliced and polyadenylated. To investigate the mechanism whereby processing factors recognize last exons containing both splicing and polyadenylation consensus elements, chimeric precursor RNAs containing a single intron and a poly(A) site were construct...
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| Veröffentlicht in: | Genes & development 1990-09, Vol.4 (9), p.1552-1559 |
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| creator | NIWA, M ROSE, S. D BERGET, S. M |
| description | The majority of vertebrate pre-mRNAs are both spliced and polyadenylated. To investigate the mechanism whereby processing factors recognize last exons containing both splicing and polyadenylation consensus elements, chimeric precursor RNAs containing a single intron and a poly(A) site were constructed and assayed for in vitro splicing and polyadenylation. Chimeric RNAs underwent splicing and polyadenylation. Both reactions occurred in a single RNA. The presence of an intron enhanced the rate of polyadenylation at a downstream poly(A) site. The extent of stimulation varied from two- to fivefold, depending on the magnesium concentration. Maximal stimulation of polyadenylation by an upstream intron required a 3' splice site but not a 5' splice site, suggesting that the structure of the terminal exon was more important than the presence of a complete upstream intron. We suggest that splicing and polyadenylation factors interact to recognize terminal, poly(A) site-containing exons. Such interaction may explain why all known intron-containing eukaryotic pre-mRNAs generate their 3' ends by polyadenylation. |
| doi_str_mv | 10.1101/gad.4.9.1552 |
| format | Article |
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We suggest that splicing and polyadenylation factors interact to recognize terminal, poly(A) site-containing exons. Such interaction may explain why all known intron-containing eukaryotic pre-mRNAs generate their 3' ends by polyadenylation.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.4.9.1552</identifier><identifier>PMID: 1701407</identifier><identifier>CODEN: GEDEEP</identifier><language>eng</language><publisher>Cold Spring Harbor, NY: Cold Spring Harbor Laboratory</publisher><subject>Base Sequence ; Biological and medical sciences ; Cell Nucleus - metabolism ; Cell-Free System ; Exons - physiology ; Fundamental and applied biological sciences. 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D</creatorcontrib><creatorcontrib>BERGET, S. M</creatorcontrib><title>In vitro polyadenylation is stimulated by the presence of an upstream intron</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>The majority of vertebrate pre-mRNAs are both spliced and polyadenylated. To investigate the mechanism whereby processing factors recognize last exons containing both splicing and polyadenylation consensus elements, chimeric precursor RNAs containing a single intron and a poly(A) site were constructed and assayed for in vitro splicing and polyadenylation. Chimeric RNAs underwent splicing and polyadenylation. Both reactions occurred in a single RNA. The presence of an intron enhanced the rate of polyadenylation at a downstream poly(A) site. The extent of stimulation varied from two- to fivefold, depending on the magnesium concentration. Maximal stimulation of polyadenylation by an upstream intron required a 3' splice site but not a 5' splice site, suggesting that the structure of the terminal exon was more important than the presence of a complete upstream intron. We suggest that splicing and polyadenylation factors interact to recognize terminal, poly(A) site-containing exons. Such interaction may explain why all known intron-containing eukaryotic pre-mRNAs generate their 3' ends by polyadenylation.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell-Free System</subject><subject>Exons - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Introns - genetics</subject><subject>Introns - physiology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Poly A - biosynthesis</subject><subject>Regulatory Sequences, Nucleic Acid - genetics</subject><subject>RNA - genetics</subject><subject>RNA Precursors - metabolism</subject><subject>RNA Processing, Post-Transcriptional - genetics</subject><subject>RNA Splicing - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Introns - genetics</topic><topic>Introns - physiology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Poly A - biosynthesis</topic><topic>Regulatory Sequences, Nucleic Acid - genetics</topic><topic>RNA - genetics</topic><topic>RNA Precursors - metabolism</topic><topic>RNA Processing, Post-Transcriptional - genetics</topic><topic>RNA Splicing - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Vertebrata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NIWA, M</creatorcontrib><creatorcontrib>ROSE, S. D</creatorcontrib><creatorcontrib>BERGET, S. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro polyadenylation is stimulated by the presence of an upstream intron</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>1990-09</date><risdate>1990</risdate><volume>4</volume><issue>9</issue><spage>1552</spage><epage>1559</epage><pages>1552-1559</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><coden>GEDEEP</coden><abstract>The majority of vertebrate pre-mRNAs are both spliced and polyadenylated. To investigate the mechanism whereby processing factors recognize last exons containing both splicing and polyadenylation consensus elements, chimeric precursor RNAs containing a single intron and a poly(A) site were constructed and assayed for in vitro splicing and polyadenylation. Chimeric RNAs underwent splicing and polyadenylation. Both reactions occurred in a single RNA. The presence of an intron enhanced the rate of polyadenylation at a downstream poly(A) site. The extent of stimulation varied from two- to fivefold, depending on the magnesium concentration. Maximal stimulation of polyadenylation by an upstream intron required a 3' splice site but not a 5' splice site, suggesting that the structure of the terminal exon was more important than the presence of a complete upstream intron. We suggest that splicing and polyadenylation factors interact to recognize terminal, poly(A) site-containing exons. Such interaction may explain why all known intron-containing eukaryotic pre-mRNAs generate their 3' ends by polyadenylation.</abstract><cop>Cold Spring Harbor, NY</cop><pub>Cold Spring Harbor Laboratory</pub><pmid>1701407</pmid><doi>10.1101/gad.4.9.1552</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0890-9369 |
| ispartof | Genes & development, 1990-09, Vol.4 (9), p.1552-1559 |
| issn | 0890-9369 1549-5477 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Base Sequence Biological and medical sciences Cell Nucleus - metabolism Cell-Free System Exons - physiology Fundamental and applied biological sciences. Psychology HeLa Cells Humans Introns - genetics Introns - physiology Molecular and cellular biology Molecular genetics Molecular Sequence Data Poly A - biosynthesis Regulatory Sequences, Nucleic Acid - genetics RNA - genetics RNA Precursors - metabolism RNA Processing, Post-Transcriptional - genetics RNA Splicing - genetics RNA, Messenger - biosynthesis Transcription. Transcription factor. Splicing. Rna processing Vertebrata |
| title | In vitro polyadenylation is stimulated by the presence of an upstream intron |
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