The surface of articular cartilage contains a progenitor cell population
It is becoming increasingly apparent that articular cartilage growth is achieved by apposition from the articular surface. For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report...
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| Veröffentlicht in: | Journal of cell science 2004-02, Vol.117 (Pt 6), p.889-897 |
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| container_issue | Pt 6 |
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| container_title | Journal of cell science |
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| creator | Dowthwaite, Gary P Bishop, Joanna C Redman, Samantha N Khan, Ilyas M Rooney, Paul Evans, Darrell J R Haughton, Laura Bayram, Zubeyde Boyer, Sam Thomson, Brian Wolfe, Michael S Archer, Charles W |
| description | It is becoming increasingly apparent that articular cartilage growth is achieved by apposition from the articular surface. For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. The identification of a chondrocyte subpopulation with progenitor-like characteristics will allow for advances in our understanding of both cartilage growth and maintenance as well as provide novel solutions to articular cartilage repair. |
| doi_str_mv | 10.1242/jcs.00912 |
| format | Article |
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For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. 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For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. The identification of a chondrocyte subpopulation with progenitor-like characteristics will allow for advances in our understanding of both cartilage growth and maintenance as well as provide novel solutions to articular cartilage repair.</description><subject>Amyloid Precursor Protein Secretases</subject><subject>Animals</subject><subject>Bone and Bones - embryology</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - growth & development</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cattle</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - physiology</subject><subject>Chick Embryo</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - metabolism</subject><subject>Endopeptidases - metabolism</subject><subject>Fibronectins - pharmacology</subject><subject>Genetic Vectors</subject><subject>Integrin alpha5beta1 - metabolism</subject><subject>Protease Inhibitors - pharmacology</subject><subject>Receptor, Notch1</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Recombinant Proteins</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Tendons - embryology</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transduction, Genetic</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFLwzAYhoMobk4P_gHJSfDQmS9Jm-QoQ50w8DLP5WuWzI6uqUl78N_buYFHT98L38PLy0PILbA5cMkfdzbNGTPAz8gUpFKZAaHOyZQxDpnJhZiQq5R2jDHFjbokkxEqODA1Jcv1p6NpiB6to8FTjH1thwYjtYfY4NZRG9oe6zZRpF0MW9fWfRj_rmloF7oR7uvQXpMLj01yN6c7Ix8vz-vFMlu9v74tnlaZFZr1WVVUyGHDALTUkhv0lWeoAGUhhNVFIaVCzlADr9B7bXJbiaIw3OQbYUQuZuT-2DtO-Rpc6st9nQ5bsHVhSKVmkGtl1L8gKAWGSzOCD0fQxpBSdL7sYr3H-F0CKw9-y9Fv-et3ZO9OpUO1d5s_8iRU_ABG2XT2</recordid><startdate>20040222</startdate><enddate>20040222</enddate><creator>Dowthwaite, Gary P</creator><creator>Bishop, Joanna C</creator><creator>Redman, Samantha N</creator><creator>Khan, Ilyas M</creator><creator>Rooney, Paul</creator><creator>Evans, Darrell J R</creator><creator>Haughton, Laura</creator><creator>Bayram, Zubeyde</creator><creator>Boyer, Sam</creator><creator>Thomson, Brian</creator><creator>Wolfe, Michael S</creator><creator>Archer, Charles W</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>20040222</creationdate><title>The surface of articular cartilage contains a progenitor cell population</title><author>Dowthwaite, Gary P ; 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For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Company of Biologists |
| subjects | Amyloid Precursor Protein Secretases Animals Bone and Bones - embryology Cartilage, Articular - cytology Cartilage, Articular - growth & development Cartilage, Articular - metabolism Cattle Cell Adhesion - drug effects Cell Adhesion - physiology Cell Division - drug effects Cell Division - physiology Chick Embryo Chondrocytes - cytology Chondrocytes - metabolism Endopeptidases - metabolism Fibronectins - pharmacology Genetic Vectors Integrin alpha5beta1 - metabolism Protease Inhibitors - pharmacology Receptor, Notch1 Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Recombinant Proteins Stem Cells - cytology Stem Cells - metabolism Tendons - embryology Transcription Factors - genetics Transcription Factors - metabolism Transduction, Genetic |
| title | The surface of articular cartilage contains a progenitor cell population |
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