Factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from Flavobacterium multivorum
Aims: To study the effect of intermediates of the tricarboxylic acid (TCA) cycle on the production of zeaxanthin from Flavobacterium multivorum in order to optimize production of this xanthophyll carotenoid. Methods and Results: The concentration of selected TCA cycle intermediates (malic acid, is...
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| Veröffentlicht in: | Journal of applied microbiology 2004-03, Vol.96 (3), p.623-629 |
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| creator | Bhosale, P Larson, A.J Bernstein, P.S |
| description | Aims: To study the effect of intermediates of the tricarboxylic acid (TCA) cycle on the production of zeaxanthin from Flavobacterium multivorum in order to optimize production of this xanthophyll carotenoid.
Methods and Results: The concentration of selected TCA cycle intermediates (malic acid, isocitric acid and α‐ketoglutarate) was optimized in shake flask culture, using a statistical two‐level, three‐variable factorial approach. The carotenoid production profile was also studied in the optimized medium at various growth phases. Optimized medium resulted in a sixfold increase in volumetric production of zeaxanthin (10·65 ± 0·63 μg ml−1) using malic acid (6·02 mm), isocitric acid (6·20 mm) and α‐ketoglutarate (0·02 mm). The majority of zeaxanthin was produced in the late logarithmic growth phase whereas a substantial amount of β‐cryptoxanthin and β‐carotene were observed in the early logarithmic phase.
Significance and Impact of the Study: This study demonstrates improvement of zeaxanthin production from F. multivorum which might aid in the commercialization of zeaxanthin production from this microbe. |
| doi_str_mv | 10.1111/j.1365-2672.2004.02197.x |
| format | Article |
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Methods and Results: The concentration of selected TCA cycle intermediates (malic acid, isocitric acid and α‐ketoglutarate) was optimized in shake flask culture, using a statistical two‐level, three‐variable factorial approach. The carotenoid production profile was also studied in the optimized medium at various growth phases. Optimized medium resulted in a sixfold increase in volumetric production of zeaxanthin (10·65 ± 0·63 μg ml−1) using malic acid (6·02 mm), isocitric acid (6·20 mm) and α‐ketoglutarate (0·02 mm). The majority of zeaxanthin was produced in the late logarithmic growth phase whereas a substantial amount of β‐cryptoxanthin and β‐carotene were observed in the early logarithmic phase.
Significance and Impact of the Study: This study demonstrates improvement of zeaxanthin production from F. multivorum which might aid in the commercialization of zeaxanthin production from this microbe.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2004.02197.x</identifier><identifier>PMID: 14962143</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>beta Carotene - analogs & derivatives ; beta Carotene - analysis ; beta Carotene - biosynthesis ; beta Carotene - therapeutic use ; Biological and medical sciences ; Bioreactors ; Chromatography, High Pressure Liquid ; Citric Acid Cycle ; Culture Media ; Dietary Supplements ; factorial design ; Flavobacterium - growth & development ; Flavobacterium - metabolism ; Flavobacterium multivorum ; Fundamental and applied biological sciences. Psychology ; Humans ; Isocitrates - metabolism ; Ketoglutaric Acids - metabolism ; Macular Degeneration - drug therapy ; Malates - metabolism ; Microbiology ; Neoplasms - drug therapy ; TCA cycle intermediates ; Xanthophylls ; zeaxanthin ; Zeaxanthins ; β‐carotene ; β‐cryptoxanthin</subject><ispartof>Journal of applied microbiology, 2004-03, Vol.96 (3), p.623-629</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5937-208f79919abf4b7423e770c7ecf6449ce3ff8a8c8e2f58dba2814d6d995c50213</citedby><cites>FETCH-LOGICAL-c5937-208f79919abf4b7423e770c7ecf6449ce3ff8a8c8e2f58dba2814d6d995c50213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2004.02197.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2004.02197.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15521218$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14962143$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhosale, P</creatorcontrib><creatorcontrib>Larson, A.J</creatorcontrib><creatorcontrib>Bernstein, P.S</creatorcontrib><title>Factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from Flavobacterium multivorum</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: To study the effect of intermediates of the tricarboxylic acid (TCA) cycle on the production of zeaxanthin from Flavobacterium multivorum in order to optimize production of this xanthophyll carotenoid.
Methods and Results: The concentration of selected TCA cycle intermediates (malic acid, isocitric acid and α‐ketoglutarate) was optimized in shake flask culture, using a statistical two‐level, three‐variable factorial approach. The carotenoid production profile was also studied in the optimized medium at various growth phases. Optimized medium resulted in a sixfold increase in volumetric production of zeaxanthin (10·65 ± 0·63 μg ml−1) using malic acid (6·02 mm), isocitric acid (6·20 mm) and α‐ketoglutarate (0·02 mm). The majority of zeaxanthin was produced in the late logarithmic growth phase whereas a substantial amount of β‐cryptoxanthin and β‐carotene were observed in the early logarithmic phase.
Significance and Impact of the Study: This study demonstrates improvement of zeaxanthin production from F. multivorum which might aid in the commercialization of zeaxanthin production from this microbe.</description><subject>beta Carotene - analogs & derivatives</subject><subject>beta Carotene - analysis</subject><subject>beta Carotene - biosynthesis</subject><subject>beta Carotene - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Citric Acid Cycle</subject><subject>Culture Media</subject><subject>Dietary Supplements</subject><subject>factorial design</subject><subject>Flavobacterium - growth & development</subject><subject>Flavobacterium - metabolism</subject><subject>Flavobacterium multivorum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Isocitrates - metabolism</subject><subject>Ketoglutaric Acids - metabolism</subject><subject>Macular Degeneration - drug therapy</subject><subject>Malates - metabolism</subject><subject>Microbiology</subject><subject>Neoplasms - drug therapy</subject><subject>TCA cycle intermediates</subject><subject>Xanthophylls</subject><subject>zeaxanthin</subject><subject>Zeaxanthins</subject><subject>β‐carotene</subject><subject>β‐cryptoxanthin</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhiMEoqXwCmAhlVsW23Fi-8ChqlhaVMQBerYmjg1eOfFiJ2XTO--Ns7uiEhfwZUaa758Zze-iQASvSH5vNytSNXVJG05XFGO2wpRIvto9Kk7_FB7vc1bWmNOT4llKG4xJhevmaXFCmGwoYdVp8WsNegzRgUcwgJ-TSyhYNEanIbZhN3unEWjXIT1rb5AbRhN70zkYTUI2RBS2o-vdPYwuDIv03sAOhvG7G9A2hm7S-4KNoUdrD3ehzQNNdFOP-smP7i7EqX9ePLHgk3lxjGfF7fr918ur8ubzh-vLi5tS17LiJcXCcimJhNayljNaGc6x5kbbhjGpTWWtAKGFobYWXQtUENY1nZS1rvOJqrPizaFv3uzHZNKoepe08R4GE6akBCZ1RRj-J0i4JKKhC_j6L3ATppgvmRStqGSsEU2GxAHSMaQUjVXb6HqIsyJYLYaqjVp8U4tvajFU7Q1Vuyx9eew_tfnsD8Kjgxk4PwKQNHgbYdAuPXB1TQklInPvDtxP58383wuojxeflizrXx30FoKCbzHPuP1Clx-FpRSMN9VvLJbHww</recordid><startdate>200403</startdate><enddate>200403</enddate><creator>Bhosale, P</creator><creator>Larson, A.J</creator><creator>Bernstein, P.S</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200403</creationdate><title>Factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from Flavobacterium multivorum</title><author>Bhosale, P ; Larson, A.J ; Bernstein, P.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5937-208f79919abf4b7423e770c7ecf6449ce3ff8a8c8e2f58dba2814d6d995c50213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>beta Carotene - analogs & derivatives</topic><topic>beta Carotene - analysis</topic><topic>beta Carotene - biosynthesis</topic><topic>beta Carotene - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Citric Acid Cycle</topic><topic>Culture Media</topic><topic>Dietary Supplements</topic><topic>factorial design</topic><topic>Flavobacterium - growth & development</topic><topic>Flavobacterium - metabolism</topic><topic>Flavobacterium multivorum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Isocitrates - metabolism</topic><topic>Ketoglutaric Acids - metabolism</topic><topic>Macular Degeneration - drug therapy</topic><topic>Malates - metabolism</topic><topic>Microbiology</topic><topic>Neoplasms - drug therapy</topic><topic>TCA cycle intermediates</topic><topic>Xanthophylls</topic><topic>zeaxanthin</topic><topic>Zeaxanthins</topic><topic>β‐carotene</topic><topic>β‐cryptoxanthin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhosale, P</creatorcontrib><creatorcontrib>Larson, A.J</creatorcontrib><creatorcontrib>Bernstein, P.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhosale, P</au><au>Larson, A.J</au><au>Bernstein, P.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from Flavobacterium multivorum</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2004-03</date><risdate>2004</risdate><volume>96</volume><issue>3</issue><spage>623</spage><epage>629</epage><pages>623-629</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims: To study the effect of intermediates of the tricarboxylic acid (TCA) cycle on the production of zeaxanthin from Flavobacterium multivorum in order to optimize production of this xanthophyll carotenoid.
Methods and Results: The concentration of selected TCA cycle intermediates (malic acid, isocitric acid and α‐ketoglutarate) was optimized in shake flask culture, using a statistical two‐level, three‐variable factorial approach. The carotenoid production profile was also studied in the optimized medium at various growth phases. Optimized medium resulted in a sixfold increase in volumetric production of zeaxanthin (10·65 ± 0·63 μg ml−1) using malic acid (6·02 mm), isocitric acid (6·20 mm) and α‐ketoglutarate (0·02 mm). The majority of zeaxanthin was produced in the late logarithmic growth phase whereas a substantial amount of β‐cryptoxanthin and β‐carotene were observed in the early logarithmic phase.
Significance and Impact of the Study: This study demonstrates improvement of zeaxanthin production from F. multivorum which might aid in the commercialization of zeaxanthin production from this microbe.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>14962143</pmid><doi>10.1111/j.1365-2672.2004.02197.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied microbiology, 2004-03, Vol.96 (3), p.623-629 |
| issn | 1364-5072 1365-2672 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | beta Carotene - analogs & derivatives beta Carotene - analysis beta Carotene - biosynthesis beta Carotene - therapeutic use Biological and medical sciences Bioreactors Chromatography, High Pressure Liquid Citric Acid Cycle Culture Media Dietary Supplements factorial design Flavobacterium - growth & development Flavobacterium - metabolism Flavobacterium multivorum Fundamental and applied biological sciences. Psychology Humans Isocitrates - metabolism Ketoglutaric Acids - metabolism Macular Degeneration - drug therapy Malates - metabolism Microbiology Neoplasms - drug therapy TCA cycle intermediates Xanthophylls zeaxanthin Zeaxanthins β‐carotene β‐cryptoxanthin |
| title | Factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from Flavobacterium multivorum |
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