Role of calf-adapted Escherichia coli in maintenance of antimicrobial drug resistance in dairy calves
The prevalence of antimicrobial drug-resistant bacteria is typically highest in younger animals, and prevalence is not necessarily related to recent use of antimicrobial drugs. In dairy cattle, we hypothesize that antimicrobial drug-resistant, neonate-adapted bacteria are responsible for the observe...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2004-02, Vol.70 (2), p.752-757 |
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| description | The prevalence of antimicrobial drug-resistant bacteria is typically highest in younger animals, and prevalence is not necessarily related to recent use of antimicrobial drugs. In dairy cattle, we hypothesize that antimicrobial drug-resistant, neonate-adapted bacteria are responsible for the observed high frequencies of resistant Escherichia coli in calves. To explore this issue, we examined the age distribution of antimicrobial drug-resistant E. coli from Holstein cattle at a local dairy and conducted an experiment to determine if low doses of oxytetracycline affected the prevalence of antimicrobial drug-resistant E. coli. Isolates resistant to tetracycline (>4 micrograms/ml) were more prevalent in 12 micrograms/ml), sulfadiazine (>512 micrograms/ml), and tetracycline (>4 micrograms/ml) (SSuT), and this resistance pattern was most prevalent during the period when calves were on milk diets. To determine if prevalence was a function of differential fitness, we orally inoculated animals with nalidixic acid-resistant strains of SSuT E. coli and susceptible E. coli. Shedding of SSuT E. coli was significantly greater than that of susceptible strains in neonatal calves (P < 0.001), whereas there was no difference in older animals (P = 0.5). These data support the hypothesis that active selection for traits linked to the SSuT phenotype are responsible for maintaining drug-resistant E. coli in this population of dairy calves. |
| doi_str_mv | 10.1128/AEM.70.2.752-757.2004 |
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In dairy cattle, we hypothesize that antimicrobial drug-resistant, neonate-adapted bacteria are responsible for the observed high frequencies of resistant Escherichia coli in calves. To explore this issue, we examined the age distribution of antimicrobial drug-resistant E. coli from Holstein cattle at a local dairy and conducted an experiment to determine if low doses of oxytetracycline affected the prevalence of antimicrobial drug-resistant E. coli. Isolates resistant to tetracycline (>4 micrograms/ml) were more prevalent in <3-month-old calves (79%) compared with lactating cows (14%). In an experimental trial where calves received diets supplemented with or without oxytetracycline, the prevalence of tetracycline-resistant E. coli was slightly higher for the latter group (P = 0.039), indicating that drug use was not required to maintain a high prevalence of resistant E. coli. The most common resistance pattern among calf E. coli isolates included resistance to streptomycin (>12 micrograms/ml), sulfadiazine (>512 micrograms/ml), and tetracycline (>4 micrograms/ml) (SSuT), and this resistance pattern was most prevalent during the period when calves were on milk diets. To determine if prevalence was a function of differential fitness, we orally inoculated animals with nalidixic acid-resistant strains of SSuT E. coli and susceptible E. coli. Shedding of SSuT E. coli was significantly greater than that of susceptible strains in neonatal calves (P < 0.001), whereas there was no difference in older animals (P = 0.5). These data support the hypothesis that active selection for traits linked to the SSuT phenotype are responsible for maintaining drug-resistant E. coli in this population of dairy calves.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.70.2.752-757.2004</identifier><identifier>PMID: 14766551</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>age ; Aging - physiology ; ampicillin ; Animals ; Anti-Bacterial Agents - pharmacology ; antibiotic resistance ; Bacteria ; Biological and medical sciences ; calves ; Cattle ; cattle diseases ; Cattle Diseases - microbiology ; chloramphenicol ; dairy cattle ; Dairying ; digestive system diseases ; Drug resistance ; Drug Resistance, Bacterial ; drug therapy ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - veterinary ; Escherichia infections ; feed supplements ; Fundamental and applied biological sciences. Psychology ; intestinal microorganisms ; Microbial Sensitivity Tests ; Microbiology ; oral administration ; oxytetracycline ; Oxytetracycline - pharmacology ; Prevalence ; Public Health Microbiology ; streptomycin ; sulfadiazine ; sulfamethoxazole ; tetracycline</subject><ispartof>Applied and Environmental Microbiology, 2004-02, Vol.70 (2), p.752-757</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Feb 2004</rights><rights>Copyright © 2004, American Society for Microbiology 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c595t-c1f577d2c643afed62a38ebfff7adeb3d54157668af56628e3b256a3074509733</citedby><cites>FETCH-LOGICAL-c595t-c1f577d2c643afed62a38ebfff7adeb3d54157668af56628e3b256a3074509733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC348837/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC348837/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15595100$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14766551$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khachatryan, A.R</creatorcontrib><creatorcontrib>Hancock, D.D</creatorcontrib><creatorcontrib>Besser, T.E</creatorcontrib><creatorcontrib>Call, D.R</creatorcontrib><title>Role of calf-adapted Escherichia coli in maintenance of antimicrobial drug resistance in dairy calves</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The prevalence of antimicrobial drug-resistant bacteria is typically highest in younger animals, and prevalence is not necessarily related to recent use of antimicrobial drugs. In dairy cattle, we hypothesize that antimicrobial drug-resistant, neonate-adapted bacteria are responsible for the observed high frequencies of resistant Escherichia coli in calves. To explore this issue, we examined the age distribution of antimicrobial drug-resistant E. coli from Holstein cattle at a local dairy and conducted an experiment to determine if low doses of oxytetracycline affected the prevalence of antimicrobial drug-resistant E. coli. Isolates resistant to tetracycline (>4 micrograms/ml) were more prevalent in <3-month-old calves (79%) compared with lactating cows (14%). In an experimental trial where calves received diets supplemented with or without oxytetracycline, the prevalence of tetracycline-resistant E. coli was slightly higher for the latter group (P = 0.039), indicating that drug use was not required to maintain a high prevalence of resistant E. coli. The most common resistance pattern among calf E. coli isolates included resistance to streptomycin (>12 micrograms/ml), sulfadiazine (>512 micrograms/ml), and tetracycline (>4 micrograms/ml) (SSuT), and this resistance pattern was most prevalent during the period when calves were on milk diets. To determine if prevalence was a function of differential fitness, we orally inoculated animals with nalidixic acid-resistant strains of SSuT E. coli and susceptible E. coli. Shedding of SSuT E. coli was significantly greater than that of susceptible strains in neonatal calves (P < 0.001), whereas there was no difference in older animals (P = 0.5). These data support the hypothesis that active selection for traits linked to the SSuT phenotype are responsible for maintaining drug-resistant E. coli in this population of dairy calves.</description><subject>age</subject><subject>Aging - physiology</subject><subject>ampicillin</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiotic resistance</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>calves</subject><subject>Cattle</subject><subject>cattle diseases</subject><subject>Cattle Diseases - microbiology</subject><subject>chloramphenicol</subject><subject>dairy cattle</subject><subject>Dairying</subject><subject>digestive system diseases</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial</subject><subject>drug therapy</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Escherichia infections</subject><subject>feed supplements</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>intestinal microorganisms</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>oral administration</subject><subject>oxytetracycline</subject><subject>Oxytetracycline - pharmacology</subject><subject>Prevalence</subject><subject>Public Health Microbiology</subject><subject>streptomycin</subject><subject>sulfadiazine</subject><subject>sulfamethoxazole</subject><subject>tetracycline</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0stu1DAUBmALgehQeAQgQoJdBl_i26KLqhouUhES0LV14tgTV0k82ElR3x5PZ9QCGxaRF_nO8bF_I_SS4DUhVL0_33xZS7yma8lpLblcU4ybR2hFsFY1Z0w8RiuMta4pbfAJepbzNS4CC_UUnZBGCsE5WSH3LQ6uir6yMPgaOtjNrqs22fYuBdsHqGwcQhWmaoQwzW6Cyd55mOYwBptiG2CourRsq-RyyPMdKL6DkG73bW9cfo6eeBiye3FcT9HVh82Pi0_15dePny_OL2vLNZ9rSzyXsqNWNAy86wQFplzrvZfQuZZ1vCG8TK7AcyGocqylXADDsuFYS8ZO0dmh725pR9dZN80JBrNLYYR0ayIE8_efKfRmG28Ma5RistS_O9an-HNxeTZjyNYNA0wuLtkoTBqtpfovJJpKLCkt8M0_8DouaSqXYCguh9aU6YL4AZXrzDk5fz8xwWaftilpG4kNNSXt8kmzT7vUvfrzuA9Vx3gLeHsEkPcBpxJOyA-OlwkIxsVVB9eHbf8rJGcgjwbceL9pIa8PxEM0sE2lzdV3igkrj4xxIjT7DWTBx_I</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Khachatryan, A.R</creator><creator>Hancock, D.D</creator><creator>Besser, T.E</creator><creator>Call, D.R</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20040201</creationdate><title>Role of calf-adapted Escherichia coli in maintenance of antimicrobial drug resistance in dairy calves</title><author>Khachatryan, A.R ; Hancock, D.D ; Besser, T.E ; Call, D.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c595t-c1f577d2c643afed62a38ebfff7adeb3d54157668af56628e3b256a3074509733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>age</topic><topic>Aging - physiology</topic><topic>ampicillin</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiotic resistance</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>calves</topic><topic>Cattle</topic><topic>cattle diseases</topic><topic>Cattle Diseases - microbiology</topic><topic>chloramphenicol</topic><topic>dairy cattle</topic><topic>Dairying</topic><topic>digestive system diseases</topic><topic>Drug resistance</topic><topic>Drug Resistance, Bacterial</topic><topic>drug therapy</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Escherichia infections</topic><topic>feed supplements</topic><topic>Fundamental and applied biological sciences. 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In dairy cattle, we hypothesize that antimicrobial drug-resistant, neonate-adapted bacteria are responsible for the observed high frequencies of resistant Escherichia coli in calves. To explore this issue, we examined the age distribution of antimicrobial drug-resistant E. coli from Holstein cattle at a local dairy and conducted an experiment to determine if low doses of oxytetracycline affected the prevalence of antimicrobial drug-resistant E. coli. Isolates resistant to tetracycline (>4 micrograms/ml) were more prevalent in <3-month-old calves (79%) compared with lactating cows (14%). In an experimental trial where calves received diets supplemented with or without oxytetracycline, the prevalence of tetracycline-resistant E. coli was slightly higher for the latter group (P = 0.039), indicating that drug use was not required to maintain a high prevalence of resistant E. coli. The most common resistance pattern among calf E. coli isolates included resistance to streptomycin (>12 micrograms/ml), sulfadiazine (>512 micrograms/ml), and tetracycline (>4 micrograms/ml) (SSuT), and this resistance pattern was most prevalent during the period when calves were on milk diets. To determine if prevalence was a function of differential fitness, we orally inoculated animals with nalidixic acid-resistant strains of SSuT E. coli and susceptible E. coli. Shedding of SSuT E. coli was significantly greater than that of susceptible strains in neonatal calves (P < 0.001), whereas there was no difference in older animals (P = 0.5). These data support the hypothesis that active selection for traits linked to the SSuT phenotype are responsible for maintaining drug-resistant E. coli in this population of dairy calves.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>14766551</pmid><doi>10.1128/AEM.70.2.752-757.2004</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | age Aging - physiology ampicillin Animals Anti-Bacterial Agents - pharmacology antibiotic resistance Bacteria Biological and medical sciences calves Cattle cattle diseases Cattle Diseases - microbiology chloramphenicol dairy cattle Dairying digestive system diseases Drug resistance Drug Resistance, Bacterial drug therapy Escherichia coli Escherichia coli - drug effects Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia infections feed supplements Fundamental and applied biological sciences. Psychology intestinal microorganisms Microbial Sensitivity Tests Microbiology oral administration oxytetracycline Oxytetracycline - pharmacology Prevalence Public Health Microbiology streptomycin sulfadiazine sulfamethoxazole tetracycline |
| title | Role of calf-adapted Escherichia coli in maintenance of antimicrobial drug resistance in dairy calves |
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