Dual role of sequence-dependent DNA curvature in nucleosome stability: the critical test of highly bent Crithidia fasciculata DNA tract
In spite of the knowledge of the nucleosome molecular structure, the role of DNA intrinsic curvature in determining nucleosome stabilization is still an open question. In this paper, we describe a general model that allows the prediction of the nucleosome stability, tested on 83 different DNA sequen...
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| Veröffentlicht in: | Biophysical chemistry 2004-01, Vol.107 (1), p.7-17 |
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| container_title | Biophysical chemistry |
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| creator | Scipioni, Anita Pisano, Sabrina Anselmi, Claudio Savino, Maria De Santis, Pasquale |
| description | In spite of the knowledge of the nucleosome molecular structure, the role of DNA intrinsic curvature in determining nucleosome stabilization is still an open question. In this paper, we describe a general model that allows the prediction of the nucleosome stability, tested on 83 different DNA sequences, in surprising good agreement with the experimental data, carried out in ours as well as in many other laboratories. The model is based on the dual role of DNA curvature in nucleosome thermodynamic stabilization. A critical test is the evaluation of the nucleosome free energy relative to a Crithidia fasciculata kinetoplast DNA fragment, which represents the most curved DNA found so far in biological systems and, therefore, is generally believed to form a highly stable nucleosome. |
| doi_str_mv | 10.1016/S0301-4622(03)00214-X |
| format | Article |
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| subjects | Animals Base Sequence Crithidia fasciculata - genetics DNA, Protozoan - chemistry Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Nucleosomes - chemistry Nucleosomes - ultrastructure Thermodynamics |
| title | Dual role of sequence-dependent DNA curvature in nucleosome stability: the critical test of highly bent Crithidia fasciculata DNA tract |
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