Clonal proliferation of T-Cell large granular lymphocytes
Background T‐cell large granular lymphocyte (T‐LGL) leukemia is a disorder only rarely reported in children. We diagnosed a new case of a clonal T‐LGL proliferation in a 6‐year‐old girl presenting with severe neutropenia and pure red blood cell aplasia (PRCA). Procedure Flow cytometric analysis incl...
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| Veröffentlicht in: | Pediatric Blood & Cancer 2004-03, Vol.42 (3), p.275-277 |
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| container_title | Pediatric Blood & Cancer |
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| creator | Boeckx, Nancy Uyttebroeck, Anne Langerak, Anton W. Brusselmans, Caroline Goossens, Willy Bossuyt, Xavier |
| description | Background
T‐cell large granular lymphocyte (T‐LGL) leukemia is a disorder only rarely reported in children. We diagnosed a new case of a clonal T‐LGL proliferation in a 6‐year‐old girl presenting with severe neutropenia and pure red blood cell aplasia (PRCA).
Procedure
Flow cytometric analysis including TCR‐Vβ repertoire analysis and molecular studies using reverse transcriptase polymerase chain reaction (RT‐PCR), PCR heteroduplex analysis and GeneScan analysis were performed to investigate the clonal nature of the T‐LGL.
Results
Flow cytometric analysis revealed a Vβ3 clonal nature. Molecular studies identified a clonal Vβ3‐Cβ RT‐PCR product. Both PCR heteroduplex analysis and GeneScan analysis found clonal TCRB and TCRG gene rearrangements.
Conclusions
An underlying T‐LGL leukemia should be investigated in the diagnostic evaluation of acquired PRCA and neutropenia in young children. Both flow cytometric and molecular analyses can be used to establish the clonal nature of T‐LGL. © 2003 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/pbc.10378 |
| format | Article |
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T‐cell large granular lymphocyte (T‐LGL) leukemia is a disorder only rarely reported in children. We diagnosed a new case of a clonal T‐LGL proliferation in a 6‐year‐old girl presenting with severe neutropenia and pure red blood cell aplasia (PRCA).
Procedure
Flow cytometric analysis including TCR‐Vβ repertoire analysis and molecular studies using reverse transcriptase polymerase chain reaction (RT‐PCR), PCR heteroduplex analysis and GeneScan analysis were performed to investigate the clonal nature of the T‐LGL.
Results
Flow cytometric analysis revealed a Vβ3 clonal nature. Molecular studies identified a clonal Vβ3‐Cβ RT‐PCR product. Both PCR heteroduplex analysis and GeneScan analysis found clonal TCRB and TCRG gene rearrangements.
Conclusions
An underlying T‐LGL leukemia should be investigated in the diagnostic evaluation of acquired PRCA and neutropenia in young children. Both flow cytometric and molecular analyses can be used to establish the clonal nature of T‐LGL. © 2003 Wiley‐Liss, Inc.</description><identifier>ISSN: 1545-5009</identifier><identifier>EISSN: 1545-5017</identifier><identifier>EISSN: 1096-911X</identifier><identifier>DOI: 10.1002/pbc.10378</identifier><identifier>PMID: 14752866</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; Child ; Clone Cells - pathology ; Female ; Flow Cytometry ; Gene Rearrangement, T-Lymphocyte ; General aspects ; Humans ; Immunophenotyping ; large granular lymphocytes ; Leukemia, Lymphoid - diagnosis ; Leukemia, Lymphoid - pathology ; Lymphocyte Activation - physiology ; Medical sciences ; Molecular Diagnostic Techniques ; Neutropenia - etiology ; pure red blood cell aplasia ; Red-Cell Aplasia, Pure - etiology ; Reverse Transcriptase Polymerase Chain Reaction ; T-LGL leukemia ; T-Lymphocytes - pathology ; TCR-Vβ analysis ; Tumors</subject><ispartof>Pediatric Blood & Cancer, 2004-03, Vol.42 (3), p.275-277</ispartof><rights>Copyright © 2003 Wiley‐Liss, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2003 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4558-3d8373fc828803f669f1bd91d59df26249dfccd978626d0170f4cf485b252d63</citedby><cites>FETCH-LOGICAL-c4558-3d8373fc828803f669f1bd91d59df26249dfccd978626d0170f4cf485b252d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpbc.10378$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpbc.10378$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15461498$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14752866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boeckx, Nancy</creatorcontrib><creatorcontrib>Uyttebroeck, Anne</creatorcontrib><creatorcontrib>Langerak, Anton W.</creatorcontrib><creatorcontrib>Brusselmans, Caroline</creatorcontrib><creatorcontrib>Goossens, Willy</creatorcontrib><creatorcontrib>Bossuyt, Xavier</creatorcontrib><title>Clonal proliferation of T-Cell large granular lymphocytes</title><title>Pediatric Blood & Cancer</title><addtitle>Pediatr. Blood Cancer</addtitle><description>Background
T‐cell large granular lymphocyte (T‐LGL) leukemia is a disorder only rarely reported in children. We diagnosed a new case of a clonal T‐LGL proliferation in a 6‐year‐old girl presenting with severe neutropenia and pure red blood cell aplasia (PRCA).
Procedure
Flow cytometric analysis including TCR‐Vβ repertoire analysis and molecular studies using reverse transcriptase polymerase chain reaction (RT‐PCR), PCR heteroduplex analysis and GeneScan analysis were performed to investigate the clonal nature of the T‐LGL.
Results
Flow cytometric analysis revealed a Vβ3 clonal nature. Molecular studies identified a clonal Vβ3‐Cβ RT‐PCR product. Both PCR heteroduplex analysis and GeneScan analysis found clonal TCRB and TCRG gene rearrangements.
Conclusions
An underlying T‐LGL leukemia should be investigated in the diagnostic evaluation of acquired PRCA and neutropenia in young children. Both flow cytometric and molecular analyses can be used to establish the clonal nature of T‐LGL. © 2003 Wiley‐Liss, Inc.</description><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Clone Cells - pathology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Gene Rearrangement, T-Lymphocyte</subject><subject>General aspects</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>large granular lymphocytes</subject><subject>Leukemia, Lymphoid - diagnosis</subject><subject>Leukemia, Lymphoid - pathology</subject><subject>Lymphocyte Activation - physiology</subject><subject>Medical sciences</subject><subject>Molecular Diagnostic Techniques</subject><subject>Neutropenia - etiology</subject><subject>pure red blood cell aplasia</subject><subject>Red-Cell Aplasia, Pure - etiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>T-LGL leukemia</subject><subject>T-Lymphocytes - pathology</subject><subject>TCR-Vβ analysis</subject><subject>Tumors</subject><issn>1545-5009</issn><issn>1545-5017</issn><issn>1096-911X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtOAzEURC0EIhAo-AG0DUgUS_xYv0pYICDxFJEoLcdrhwUnu9iJIH-PISFUiOpOcWbuaADYQ_AYQYh77dAkQbhYA1uIFjSnEPH1lYayA7ZjfEkog1Rsgg4qOMWCsS0gS99MtM_a0Pja2aCndTPJGpcN8tJ6n3kdRjYbBT2ZJZn5-bh9bsx8auMO2HDaR7u7vF0wuDgflJf59V3_qjy5zk1BqchJJQgnzggsBCSOMenQsJKoorJymOEiHWMqyQXDrEq9oSuMKwQdYoorRrrgcBGbGr7NbJyqcR1NqqYntplFJSAiQgj-L4ghQxRLmcCjBWhCE2OwTrWhHuswVwiqrz1V2lN975nY_WXobDi21S-5HDABB0tAR6O9S0OZOv5ytGCokF9BvQX3Xns7__ujuj8tf17nC0cdp_Zj5dDhVTFOOFVPt311w8UjeXg8U5h8AhArmU8</recordid><startdate>200403</startdate><enddate>200403</enddate><creator>Boeckx, Nancy</creator><creator>Uyttebroeck, Anne</creator><creator>Langerak, Anton W.</creator><creator>Brusselmans, Caroline</creator><creator>Goossens, Willy</creator><creator>Bossuyt, Xavier</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200403</creationdate><title>Clonal proliferation of T-Cell large granular lymphocytes</title><author>Boeckx, Nancy ; Uyttebroeck, Anne ; Langerak, Anton W. ; Brusselmans, Caroline ; Goossens, Willy ; Bossuyt, Xavier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4558-3d8373fc828803f669f1bd91d59df26249dfccd978626d0170f4cf485b252d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Clone Cells - pathology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Gene Rearrangement, T-Lymphocyte</topic><topic>General aspects</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>large granular lymphocytes</topic><topic>Leukemia, Lymphoid - diagnosis</topic><topic>Leukemia, Lymphoid - pathology</topic><topic>Lymphocyte Activation - physiology</topic><topic>Medical sciences</topic><topic>Molecular Diagnostic Techniques</topic><topic>Neutropenia - etiology</topic><topic>pure red blood cell aplasia</topic><topic>Red-Cell Aplasia, Pure - etiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>T-LGL leukemia</topic><topic>T-Lymphocytes - pathology</topic><topic>TCR-Vβ analysis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boeckx, Nancy</creatorcontrib><creatorcontrib>Uyttebroeck, Anne</creatorcontrib><creatorcontrib>Langerak, Anton W.</creatorcontrib><creatorcontrib>Brusselmans, Caroline</creatorcontrib><creatorcontrib>Goossens, Willy</creatorcontrib><creatorcontrib>Bossuyt, Xavier</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric Blood & Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boeckx, Nancy</au><au>Uyttebroeck, Anne</au><au>Langerak, Anton W.</au><au>Brusselmans, Caroline</au><au>Goossens, Willy</au><au>Bossuyt, Xavier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clonal proliferation of T-Cell large granular lymphocytes</atitle><jtitle>Pediatric Blood & Cancer</jtitle><addtitle>Pediatr. Blood Cancer</addtitle><date>2004-03</date><risdate>2004</risdate><volume>42</volume><issue>3</issue><spage>275</spage><epage>277</epage><pages>275-277</pages><issn>1545-5009</issn><eissn>1545-5017</eissn><eissn>1096-911X</eissn><abstract>Background
T‐cell large granular lymphocyte (T‐LGL) leukemia is a disorder only rarely reported in children. We diagnosed a new case of a clonal T‐LGL proliferation in a 6‐year‐old girl presenting with severe neutropenia and pure red blood cell aplasia (PRCA).
Procedure
Flow cytometric analysis including TCR‐Vβ repertoire analysis and molecular studies using reverse transcriptase polymerase chain reaction (RT‐PCR), PCR heteroduplex analysis and GeneScan analysis were performed to investigate the clonal nature of the T‐LGL.
Results
Flow cytometric analysis revealed a Vβ3 clonal nature. Molecular studies identified a clonal Vβ3‐Cβ RT‐PCR product. Both PCR heteroduplex analysis and GeneScan analysis found clonal TCRB and TCRG gene rearrangements.
Conclusions
An underlying T‐LGL leukemia should be investigated in the diagnostic evaluation of acquired PRCA and neutropenia in young children. Both flow cytometric and molecular analyses can be used to establish the clonal nature of T‐LGL. © 2003 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>14752866</pmid><doi>10.1002/pbc.10378</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Child Clone Cells - pathology Female Flow Cytometry Gene Rearrangement, T-Lymphocyte General aspects Humans Immunophenotyping large granular lymphocytes Leukemia, Lymphoid - diagnosis Leukemia, Lymphoid - pathology Lymphocyte Activation - physiology Medical sciences Molecular Diagnostic Techniques Neutropenia - etiology pure red blood cell aplasia Red-Cell Aplasia, Pure - etiology Reverse Transcriptase Polymerase Chain Reaction T-LGL leukemia T-Lymphocytes - pathology TCR-Vβ analysis Tumors |
| title | Clonal proliferation of T-Cell large granular lymphocytes |
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