Comparison of rinderpest and peste des petits ruminants viruses using anti-nucleoprotein monoclonal antibodies
Monoclonal antibodies (MAbs) were obtained using a purified preparation of the RBOK strain of a rinderpest vaccine virus. The cytoplasmic immunofluorescent staining test showed that these clones had specificity for the nucleoprotein (N) of the virus. Six clones which immunoprecipitated the N protein...
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| Veröffentlicht in: | Veterinary microbiology 1990, Vol.25 (1), p.1-16 |
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| creator | Libeau, G. Lefevre, P.C. |
| description | Monoclonal antibodies (MAbs) were obtained using a purified preparation of the RBOK strain of a rinderpest vaccine virus. The cytoplasmic immunofluorescent staining test showed that these clones had specificity for the nucleoprotein (N) of the virus. Six clones which immunoprecipitated the N protein corroborated these results. Thirteen anti-N MAbs were used to compare geographically widespread rinderpest viruses (RPV) and peste des petits ruminants viruses (PPRV) to two other morbilliviruses, measles (MV) and canine distemper (CDV). The N protein antigen profiles of the 23 isolates determined by immunofluorescent staining and enzyme linked immunosorbent assay (ELISA) on infected cells enabled us to classify the strains into groups.
A differential identification of the morbilliviruses can be made using one MAb or associations of the MAbs. The potential to distinguish between RPV and PPRV and between virulent and avirulent strains of rinderpest is of primary interest. |
| doi_str_mv | 10.1016/0378-1135(90)90088-D |
| format | Article |
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A differential identification of the morbilliviruses can be made using one MAb or associations of the MAbs. The potential to distinguish between RPV and PPRV and between virulent and avirulent strains of rinderpest is of primary interest.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>anticorps monoclonal</subject><subject>anticuerpos monoclonales</subject><subject>Antigens, Viral - analysis</subject><subject>betail</subject><subject>Biological and medical sciences</subject><subject>elisa</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ganado</subject><subject>identificacion</subject><subject>identification</subject><subject>livestock</subject><subject>Microbiology</subject><subject>monoclonal antibodies</subject><subject>morbillivirus</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Nucleoproteins - immunology</subject><subject>peste bovina</subject><subject>peste bovine</subject><subject>Precipitin Tests</subject><subject>rinderpest</subject><subject>Rinderpest virus - immunology</subject><subject>Rinderpest virus - pathogenicity</subject><subject>test elisa</subject><subject>Vaccines, Attenuated - immunology</subject><subject>Viral Proteins - immunology</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><subject>Virulence</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKtuFfwAoFyo4BMZ2nNgXpGpbKNIKLnC2HHtSGSX2YieV-u9xuqtyA1880vtm_DyPkFcUPlCg7Ufgnawp5eKdgvcKQMr66gnZUNnxmomGPSWbR-Q5Oc_5FwA0qoUzcsZY0ynONiTs4nQwyecYqjhUyQeH6YB5rkxw1Vpg5TCXavZzrtIy-WBCqe58WnIRluzDbYFnX4fFjhgPKc7oQzXFEO0Ygxkf1D46j_kFeTaYMePL070lPz9f_9jd1PvvX77uLve15aKda8c64FyAcKoV0nHGGXVYvNPeDQNVHWW96ChyoaTkzqGRVthukBz6tgXFt-TiOLe4-b2UX-jJZ4vjaALGJWsJlMuG_x-kQqrCdQVsjqBNMeeEgz4kP5l0rynoNQ-9Lluvy9YK9EMe-qq0vT7NX_oJ3WPTKYCivz3pJlszDskE6_Pf2Yo3fD1b8ubIDSZqc1sC09_2VCkKwFtRXt2ST0cAy1rvPCadrcdg0fmEdtYu-n87_QNS1rEI</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Libeau, G.</creator><creator>Lefevre, P.C.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Comparison of rinderpest and peste des petits ruminants viruses using anti-nucleoprotein monoclonal antibodies</title><author>Libeau, G. ; Lefevre, P.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-d27033505d9658d32321de4961bdff19712b571e359883ddea8c5c7f830b66093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>anticorps monoclonal</topic><topic>anticuerpos monoclonales</topic><topic>Antigens, Viral - analysis</topic><topic>betail</topic><topic>Biological and medical sciences</topic><topic>elisa</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>ganado</topic><topic>identificacion</topic><topic>identification</topic><topic>livestock</topic><topic>Microbiology</topic><topic>monoclonal antibodies</topic><topic>morbillivirus</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Nucleoproteins - immunology</topic><topic>peste bovina</topic><topic>peste bovine</topic><topic>Precipitin Tests</topic><topic>rinderpest</topic><topic>Rinderpest virus - immunology</topic><topic>Rinderpest virus - pathogenicity</topic><topic>test elisa</topic><topic>Vaccines, Attenuated - immunology</topic><topic>Viral Proteins - immunology</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Libeau, G.</creatorcontrib><creatorcontrib>Lefevre, P.C.</creatorcontrib><creatorcontrib>Institut d' Elevage et de Medecine Veterinaire des Pays Tropicaux CIRAD, Maisons-Alfort (France)</creatorcontrib><creatorcontrib>Sveriges Lantbruksuniv., Uppsala (Sweden)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Libeau, G.</au><au>Lefevre, P.C.</au><aucorp>Institut d' Elevage et de Medecine Veterinaire des Pays Tropicaux CIRAD, Maisons-Alfort (France)</aucorp><aucorp>Sveriges Lantbruksuniv., Uppsala (Sweden)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of rinderpest and peste des petits ruminants viruses using anti-nucleoprotein monoclonal antibodies</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>1990</date><risdate>1990</risdate><volume>25</volume><issue>1</issue><spage>1</spage><epage>16</epage><pages>1-16</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Monoclonal antibodies (MAbs) were obtained using a purified preparation of the RBOK strain of a rinderpest vaccine virus. The cytoplasmic immunofluorescent staining test showed that these clones had specificity for the nucleoprotein (N) of the virus. Six clones which immunoprecipitated the N protein corroborated these results. Thirteen anti-N MAbs were used to compare geographically widespread rinderpest viruses (RPV) and peste des petits ruminants viruses (PPRV) to two other morbilliviruses, measles (MV) and canine distemper (CDV). The N protein antigen profiles of the 23 isolates determined by immunofluorescent staining and enzyme linked immunosorbent assay (ELISA) on infected cells enabled us to classify the strains into groups.
A differential identification of the morbilliviruses can be made using one MAb or associations of the MAbs. The potential to distinguish between RPV and PPRV and between virulent and avirulent strains of rinderpest is of primary interest.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2247932</pmid><doi>10.1016/0378-1135(90)90088-D</doi><tpages>16</tpages></addata></record> |
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| subjects | Animals Antibodies, Monoclonal - immunology Antibody Specificity anticorps monoclonal anticuerpos monoclonales Antigens, Viral - analysis betail Biological and medical sciences elisa Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology ganado identificacion identification livestock Microbiology monoclonal antibodies morbillivirus Morphology, structure, chemical composition, physicochemical properties Nucleoproteins - immunology peste bovina peste bovine Precipitin Tests rinderpest Rinderpest virus - immunology Rinderpest virus - pathogenicity test elisa Vaccines, Attenuated - immunology Viral Proteins - immunology Viral Vaccines - immunology Virology Virulence |
| title | Comparison of rinderpest and peste des petits ruminants viruses using anti-nucleoprotein monoclonal antibodies |
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