The evolution of function and response to arginine challenge and pregnancy of portally and systemically placed islet cell grafts in streptozotocin diabetic mice
Mice made diabetic with streptozotocin received organ cultured fetal islet cell grafts in the portal (PG) or systemic (SG) vascular beds in order to determine whether portal delivery of insulin is an important consideration in graft placement. Grafts were established more quickly in SG mice as shown...
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Veröffentlicht in: | Metabolism, clinical and experimental clinical and experimental, 1990-12, Vol.39 (12), p.1253-1258 |
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description | Mice made diabetic with streptozotocin received organ cultured fetal islet cell grafts in the portal (PG) or systemic (SG) vascular beds in order to determine whether portal delivery of insulin is an important consideration in graft placement. Grafts were established more quickly in SG mice as shown by body weight and blood glucose estimations. Intraperitoneal arginine was found to be a stronger insulin secretagogue than intravenous glucose with the doses used when serum insulin was measured 5 minutes after injection. Glucose response to an arginine challenge was initially abnormal in both groups of mice, but was identical to normal controls 4 months after transplantation, showing that engraftment is a gradual process. Mice were then mated and their response to the chronic glycemic stress of pregnancy was noted. PG and SG mice had normal fertility rates in contrast to untreated diabetics, none of which became pregnant. Offspring were normal with respect to litter size, body weight, malformation rate, and pancreatic insulin content compared with historical controls. PG and SG mothers behaved normally during pregnancy, with the exception of a raised glycosylated hemoglobin level at day 19.5 gestation (5.5% and 5.5%
v 3.7%,
P < .01). We were, therefore, unable to detect any difference between portally and systemically placed islet grafts. |
doi_str_mv | 10.1016/0026-0495(90)90179-G |
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v 3.7%,
P < .01). We were, therefore, unable to detect any difference between portally and systemically placed islet grafts.</description><identifier>ISSN: 0026-0495</identifier><identifier>EISSN: 1532-8600</identifier><identifier>DOI: 10.1016/0026-0495(90)90179-G</identifier><identifier>PMID: 2246964</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Arginine - pharmacology ; Biological and medical sciences ; Blood Glucose - analysis ; Blood Vessels ; Body Weight ; Diabetes Mellitus, Experimental - physiopathology ; Diabetes. Impaired glucose tolerance ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Female ; Glucose Tolerance Test ; Glycated Hemoglobin A - analysis ; Insulin - metabolism ; Islets of Langerhans Transplantation ; Management. Various non-drug treatments. Langerhans islet grafts ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Pancreas - metabolism ; Portal System ; Pregnancy ; Pregnancy, Animal - physiology</subject><ispartof>Metabolism, clinical and experimental, 1990-12, Vol.39 (12), p.1253-1258</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-252f3b8635b7930064e79bef62dbfdb22aa670274ecb9d9f6c9dbe33cefe58023</citedby><cites>FETCH-LOGICAL-c387t-252f3b8635b7930064e79bef62dbfdb22aa670274ecb9d9f6c9dbe33cefe58023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0026-0495(90)90179-G$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19826374$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2246964$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gillies, M.C.</creatorcontrib><creatorcontrib>Mandel, T.E.</creatorcontrib><title>The evolution of function and response to arginine challenge and pregnancy of portally and systemically placed islet cell grafts in streptozotocin diabetic mice</title><title>Metabolism, clinical and experimental</title><addtitle>Metabolism</addtitle><description>Mice made diabetic with streptozotocin received organ cultured fetal islet cell grafts in the portal (PG) or systemic (SG) vascular beds in order to determine whether portal delivery of insulin is an important consideration in graft placement. Grafts were established more quickly in SG mice as shown by body weight and blood glucose estimations. Intraperitoneal arginine was found to be a stronger insulin secretagogue than intravenous glucose with the doses used when serum insulin was measured 5 minutes after injection. Glucose response to an arginine challenge was initially abnormal in both groups of mice, but was identical to normal controls 4 months after transplantation, showing that engraftment is a gradual process. Mice were then mated and their response to the chronic glycemic stress of pregnancy was noted. PG and SG mice had normal fertility rates in contrast to untreated diabetics, none of which became pregnant. Offspring were normal with respect to litter size, body weight, malformation rate, and pancreatic insulin content compared with historical controls. PG and SG mothers behaved normally during pregnancy, with the exception of a raised glycosylated hemoglobin level at day 19.5 gestation (5.5% and 5.5%
v 3.7%,
P < .01). We were, therefore, unable to detect any difference between portally and systemically placed islet grafts.</description><subject>Animals</subject><subject>Arginine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - analysis</subject><subject>Blood Vessels</subject><subject>Body Weight</subject><subject>Diabetes Mellitus, Experimental - physiopathology</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Glucose Tolerance Test</subject><subject>Glycated Hemoglobin A - analysis</subject><subject>Insulin - metabolism</subject><subject>Islets of Langerhans Transplantation</subject><subject>Management. Various non-drug treatments. Langerhans islet grafts</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Pancreas - metabolism</subject><subject>Portal System</subject><subject>Pregnancy</subject><subject>Pregnancy, Animal - physiology</subject><issn>0026-0495</issn><issn>1532-8600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhi0EKkPhDUDyBlQWgRM748QbJFSVAakSm7K2HOd4apSxg-1UGp6GR8XJjMqOlS__RUffIeR1DR9qqMVHACYqaOT2SsJ7CXUrq90Tsqm3nFWdAHhKNo-W5-RFSj8BoG07cUEuGGuEFM2G_Lm7R4oPYZyzC54GS-3szXrXfqAR0xR8QpoD1XHvvPNIzb0eR_R7XC1TxL3X3hyX8BRiLuJxVdIxZTw4s35MozY4UJdGzNTgONJ91DYn6jxNOeKUw--QgynPwekeszO0ZPEleWb1mPDV-bwkP77c3F1_rW6_775df76tDO_aXLEts7zvBN_2reQAosFW9mgFG3o79IxpLVpgbYOml4O0wsihR84NWtx2wPgleXfqnWL4NWPK6uDSMqf2GOakOqh5B5wXY3MymhhSimjVFN1Bx6OqQS2LUQt1tVBXEtS6GLUrsTfn_rk_4PAYOm-i6G_Puk6FmI0FqUv_umXHBG8X36eTDwuMB4dRJePQF7YuoslqCO7_g_wFvXGufA</recordid><startdate>19901201</startdate><enddate>19901201</enddate><creator>Gillies, M.C.</creator><creator>Mandel, T.E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19901201</creationdate><title>The evolution of function and response to arginine challenge and pregnancy of portally and systemically placed islet cell grafts in streptozotocin diabetic mice</title><author>Gillies, M.C. ; Mandel, T.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-252f3b8635b7930064e79bef62dbfdb22aa670274ecb9d9f6c9dbe33cefe58023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Arginine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - analysis</topic><topic>Blood Vessels</topic><topic>Body Weight</topic><topic>Diabetes Mellitus, Experimental - physiopathology</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Glucose Tolerance Test</topic><topic>Glycated Hemoglobin A - analysis</topic><topic>Insulin - metabolism</topic><topic>Islets of Langerhans Transplantation</topic><topic>Management. Various non-drug treatments. Langerhans islet grafts</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Pancreas - metabolism</topic><topic>Portal System</topic><topic>Pregnancy</topic><topic>Pregnancy, Animal - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gillies, M.C.</creatorcontrib><creatorcontrib>Mandel, T.E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolism, clinical and experimental</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gillies, M.C.</au><au>Mandel, T.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The evolution of function and response to arginine challenge and pregnancy of portally and systemically placed islet cell grafts in streptozotocin diabetic mice</atitle><jtitle>Metabolism, clinical and experimental</jtitle><addtitle>Metabolism</addtitle><date>1990-12-01</date><risdate>1990</risdate><volume>39</volume><issue>12</issue><spage>1253</spage><epage>1258</epage><pages>1253-1258</pages><issn>0026-0495</issn><eissn>1532-8600</eissn><abstract>Mice made diabetic with streptozotocin received organ cultured fetal islet cell grafts in the portal (PG) or systemic (SG) vascular beds in order to determine whether portal delivery of insulin is an important consideration in graft placement. Grafts were established more quickly in SG mice as shown by body weight and blood glucose estimations. Intraperitoneal arginine was found to be a stronger insulin secretagogue than intravenous glucose with the doses used when serum insulin was measured 5 minutes after injection. Glucose response to an arginine challenge was initially abnormal in both groups of mice, but was identical to normal controls 4 months after transplantation, showing that engraftment is a gradual process. Mice were then mated and their response to the chronic glycemic stress of pregnancy was noted. PG and SG mice had normal fertility rates in contrast to untreated diabetics, none of which became pregnant. Offspring were normal with respect to litter size, body weight, malformation rate, and pancreatic insulin content compared with historical controls. PG and SG mothers behaved normally during pregnancy, with the exception of a raised glycosylated hemoglobin level at day 19.5 gestation (5.5% and 5.5%
v 3.7%,
P < .01). We were, therefore, unable to detect any difference between portally and systemically placed islet grafts.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2246964</pmid><doi>10.1016/0026-0495(90)90179-G</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Arginine - pharmacology Biological and medical sciences Blood Glucose - analysis Blood Vessels Body Weight Diabetes Mellitus, Experimental - physiopathology Diabetes. Impaired glucose tolerance Endocrine pancreas. Apud cells (diseases) Endocrinopathies Female Glucose Tolerance Test Glycated Hemoglobin A - analysis Insulin - metabolism Islets of Langerhans Transplantation Management. Various non-drug treatments. Langerhans islet grafts Medical sciences Mice Mice, Inbred BALB C Pancreas - metabolism Portal System Pregnancy Pregnancy, Animal - physiology |
title | The evolution of function and response to arginine challenge and pregnancy of portally and systemically placed islet cell grafts in streptozotocin diabetic mice |
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