High-performance liquid chromatographic separation and quantification of citric, lactic, malic, oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection
An HPLC method was developed for the determination of citric, lactic, malic, oxalic and tartaric acids by chemiluminescent detection following online irradiation with visible light. The organic acids were irradiated with visible light in the presence of Fe 3+ and UO 2 2+ to generate Fe 2+, which was...
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| Veröffentlicht in: | Journal of Chromatography A 2004-02, Vol.1026 (1), p.57-64 |
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| creator | Pérez-Ruiz, Tomás Martı́nez-Lozano, Carmen Tomás, Virginia Martı́n, Jesús |
| description | An HPLC method was developed for the determination of citric, lactic, malic, oxalic and tartaric acids by chemiluminescent detection following online irradiation with visible light. The organic acids were irradiated with visible light in the presence of Fe
3+ and UO
2
2+ to generate Fe
2+, which was determined by measuring the chemiluminescence intensity in a luminol system in the absence of added oxidant. Factors affecting the photochemical and chemiluminescence reactions were optimised so that their contribution to the total band-broadening was negligible. The chromatographic separation was performed on a C
18 column under isocratic reversed-phase conditions using 0.005
M H
2SO
4 mobile phase. The optimised method was validated with respect to linearity, precision, limits of detection and quantification, accuracy specificity and robustness. The applicability of the assay was demonstrated by analysing these compounds in real samples such as milk, fruit juices, soft drinks, wine and beer. |
| doi_str_mv | 10.1016/j.chroma.2003.10.130 |
| format | Article |
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3+ and UO
2
2+ to generate Fe
2+, which was determined by measuring the chemiluminescence intensity in a luminol system in the absence of added oxidant. Factors affecting the photochemical and chemiluminescence reactions were optimised so that their contribution to the total band-broadening was negligible. The chromatographic separation was performed on a C
18 column under isocratic reversed-phase conditions using 0.005
M H
2SO
4 mobile phase. The optimised method was validated with respect to linearity, precision, limits of detection and quantification, accuracy specificity and robustness. The applicability of the assay was demonstrated by analysing these compounds in real samples such as milk, fruit juices, soft drinks, wine and beer.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2003.10.130</identifier><identifier>PMID: 14763733</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acids - analysis ; Acids - isolation & purification ; Animals ; Beer ; Beer - analysis ; Biological and medical sciences ; Chemiluminescence detection ; Chromatography, High Pressure Liquid ; Citric Acid - analysis ; Detection, LC ; Food analysis ; Food Analysis - methods ; Food industries ; Fruit - chemistry ; Fruit juices ; Fundamental and applied biological sciences. Psychology ; General aspects ; Lactic Acid - analysis ; Luminescent Measurements ; Malates - analysis ; Methods of analysis, processing and quality control, regulation, standards ; Milk ; Milk - chemistry ; Organic acids ; Oxalic Acid - analysis ; Photochemically induced chemiluminescence detection ; Photochemistry ; Reproducibility of Results ; Sensitivity and Specificity ; Soft drinks ; Tartrates - analysis ; Wine ; Wine - analysis</subject><ispartof>Journal of Chromatography A, 2004-02, Vol.1026 (1), p.57-64</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-61c24dd824ecefc685dab6f2379dd59f8de6db5b2b7874036776d9aad96457073</citedby><cites>FETCH-LOGICAL-c478t-61c24dd824ecefc685dab6f2379dd59f8de6db5b2b7874036776d9aad96457073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021967303020296$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15480175$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14763733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pérez-Ruiz, Tomás</creatorcontrib><creatorcontrib>Martı́nez-Lozano, Carmen</creatorcontrib><creatorcontrib>Tomás, Virginia</creatorcontrib><creatorcontrib>Martı́n, Jesús</creatorcontrib><title>High-performance liquid chromatographic separation and quantification of citric, lactic, malic, oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>An HPLC method was developed for the determination of citric, lactic, malic, oxalic and tartaric acids by chemiluminescent detection following online irradiation with visible light. The organic acids were irradiated with visible light in the presence of Fe
3+ and UO
2
2+ to generate Fe
2+, which was determined by measuring the chemiluminescence intensity in a luminol system in the absence of added oxidant. Factors affecting the photochemical and chemiluminescence reactions were optimised so that their contribution to the total band-broadening was negligible. The chromatographic separation was performed on a C
18 column under isocratic reversed-phase conditions using 0.005
M H
2SO
4 mobile phase. The optimised method was validated with respect to linearity, precision, limits of detection and quantification, accuracy specificity and robustness. The applicability of the assay was demonstrated by analysing these compounds in real samples such as milk, fruit juices, soft drinks, wine and beer.</description><subject>Acids - analysis</subject><subject>Acids - isolation & purification</subject><subject>Animals</subject><subject>Beer</subject><subject>Beer - analysis</subject><subject>Biological and medical sciences</subject><subject>Chemiluminescence detection</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Citric Acid - analysis</subject><subject>Detection, LC</subject><subject>Food analysis</subject><subject>Food Analysis - methods</subject><subject>Food industries</subject><subject>Fruit - chemistry</subject><subject>Fruit juices</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Lactic Acid - analysis</subject><subject>Luminescent Measurements</subject><subject>Malates - analysis</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>Organic acids</subject><subject>Oxalic Acid - analysis</subject><subject>Photochemically induced chemiluminescence detection</subject><subject>Photochemistry</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Soft drinks</subject><subject>Tartrates - analysis</subject><subject>Wine</subject><subject>Wine - analysis</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc-K1TAUxrtQnHH0DUSz0ZW9Jk2btBtBBnWEARc663CaP7e5tE1vkoq-nw9mcluYnRA45OR3vnx8pyheEXwgmLAPp4McvJvgUGFMD7lL8ZPiGuOKlB3j9Kp4HsIJY8Ixr54VV6TmjHJKr4u_d_Y4lIv2xvkJZqnRaM-rVWgTjO7oYRmsREEv4CFaNyOYFTqvMEdrrNxaziBpo7fyPRpBxlwnGHNxv3O9zETw6eSLtCqgNdj5iAAtLsRSunGdZrQMLjo56CkJj8jrrLX_eOkmyM46SJ2dKh315f1F8dTAGPTLvd4UD18-_7y9K--_f_12--m-lDVvY8mIrGql2qrWUhvJ2kZBz0xFeadU05lWaab6pq963vIaU8Y5Ux2A6ljdpOToTfFu0128O686RDHZ5GUcYdZuDaLFhHJG6gTWGyi9C8FrIxZvJ_B_BMEib0ycxBawyBu7dClOY693_bWftHoc2teVgLc7ACEFZHzamA2PXFMnC7xJ3JuNM-AEHH1iHn5UyR0muKJdnR1-3Aid8vpltRdB2pyqsj6FKpSz__f6Dyy1x0M</recordid><startdate>20040213</startdate><enddate>20040213</enddate><creator>Pérez-Ruiz, Tomás</creator><creator>Martı́nez-Lozano, Carmen</creator><creator>Tomás, Virginia</creator><creator>Martı́n, Jesús</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040213</creationdate><title>High-performance liquid chromatographic separation and quantification of citric, lactic, malic, oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection</title><author>Pérez-Ruiz, Tomás ; Martı́nez-Lozano, Carmen ; Tomás, Virginia ; Martı́n, Jesús</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-61c24dd824ecefc685dab6f2379dd59f8de6db5b2b7874036776d9aad96457073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acids - analysis</topic><topic>Acids - isolation & purification</topic><topic>Animals</topic><topic>Beer</topic><topic>Beer - analysis</topic><topic>Biological and medical sciences</topic><topic>Chemiluminescence detection</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Citric Acid - analysis</topic><topic>Detection, LC</topic><topic>Food analysis</topic><topic>Food Analysis - methods</topic><topic>Food industries</topic><topic>Fruit - chemistry</topic><topic>Fruit juices</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Lactic Acid - analysis</topic><topic>Luminescent Measurements</topic><topic>Malates - analysis</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>Organic acids</topic><topic>Oxalic Acid - analysis</topic><topic>Photochemically induced chemiluminescence detection</topic><topic>Photochemistry</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Soft drinks</topic><topic>Tartrates - analysis</topic><topic>Wine</topic><topic>Wine - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pérez-Ruiz, Tomás</creatorcontrib><creatorcontrib>Martı́nez-Lozano, Carmen</creatorcontrib><creatorcontrib>Tomás, Virginia</creatorcontrib><creatorcontrib>Martı́n, Jesús</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pérez-Ruiz, Tomás</au><au>Martı́nez-Lozano, Carmen</au><au>Tomás, Virginia</au><au>Martı́n, Jesús</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-performance liquid chromatographic separation and quantification of citric, lactic, malic, oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2004-02-13</date><risdate>2004</risdate><volume>1026</volume><issue>1</issue><spage>57</spage><epage>64</epage><pages>57-64</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>An HPLC method was developed for the determination of citric, lactic, malic, oxalic and tartaric acids by chemiluminescent detection following online irradiation with visible light. The organic acids were irradiated with visible light in the presence of Fe
3+ and UO
2
2+ to generate Fe
2+, which was determined by measuring the chemiluminescence intensity in a luminol system in the absence of added oxidant. Factors affecting the photochemical and chemiluminescence reactions were optimised so that their contribution to the total band-broadening was negligible. The chromatographic separation was performed on a C
18 column under isocratic reversed-phase conditions using 0.005
M H
2SO
4 mobile phase. The optimised method was validated with respect to linearity, precision, limits of detection and quantification, accuracy specificity and robustness. The applicability of the assay was demonstrated by analysing these compounds in real samples such as milk, fruit juices, soft drinks, wine and beer.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>14763733</pmid><doi>10.1016/j.chroma.2003.10.130</doi><tpages>8</tpages></addata></record> |
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| subjects | Acids - analysis Acids - isolation & purification Animals Beer Beer - analysis Biological and medical sciences Chemiluminescence detection Chromatography, High Pressure Liquid Citric Acid - analysis Detection, LC Food analysis Food Analysis - methods Food industries Fruit - chemistry Fruit juices Fundamental and applied biological sciences. Psychology General aspects Lactic Acid - analysis Luminescent Measurements Malates - analysis Methods of analysis, processing and quality control, regulation, standards Milk Milk - chemistry Organic acids Oxalic Acid - analysis Photochemically induced chemiluminescence detection Photochemistry Reproducibility of Results Sensitivity and Specificity Soft drinks Tartrates - analysis Wine Wine - analysis |
| title | High-performance liquid chromatographic separation and quantification of citric, lactic, malic, oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection |
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