Microbioreactor arrays with parametric control for high-throughput experimentation
A scalable array technology for parametric control of high‐throughput cell cultivations is demonstrated. The technology makes use of commercial printed circuit board (PCB) technology, integrated circuit sensors, and an electrochemical gas generation system. We present results for an array of eight 2...
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| Veröffentlicht in: | Biotechnology and bioengineering 2004-02, Vol.85 (4), p.376-381 |
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| container_title | Biotechnology and bioengineering |
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| creator | Maharbiz, Michel M. Holtz, William J. Howe, Roger T. Keasling, Jay D. |
| description | A scalable array technology for parametric control of high‐throughput cell cultivations is demonstrated. The technology makes use of commercial printed circuit board (PCB) technology, integrated circuit sensors, and an electrochemical gas generation system. We present results for an array of eight 250 μl microbioreactors. Each bioreactor contains an independently addressable suite that provides closed‐loop temperature control, generates feed gas electrochemically, and continuously monitors optical density. The PCB technology allows for the assembly of additional off‐the‐shelf components into the microbioreactor array; we demonstrate the use of a commercial ISFET chip to continuously monitor culture pH. The electrochemical dosing system provides a powerful paradigm for reproducible gas delivery to high‐density arrays of microreactors. Growth data are presented for Escherichia coli cultured in the array with varying microaerobic conditions using electrochemically generated oxygen. Additionally, we present data on carbon dioxide generation for pH dosing. © 2003 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.10835 |
| format | Article |
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The technology makes use of commercial printed circuit board (PCB) technology, integrated circuit sensors, and an electrochemical gas generation system. We present results for an array of eight 250 μl microbioreactors. Each bioreactor contains an independently addressable suite that provides closed‐loop temperature control, generates feed gas electrochemically, and continuously monitors optical density. The PCB technology allows for the assembly of additional off‐the‐shelf components into the microbioreactor array; we demonstrate the use of a commercial ISFET chip to continuously monitor culture pH. The electrochemical dosing system provides a powerful paradigm for reproducible gas delivery to high‐density arrays of microreactors. Growth data are presented for Escherichia coli cultured in the array with varying microaerobic conditions using electrochemically generated oxygen. 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Bioeng</addtitle><description>A scalable array technology for parametric control of high‐throughput cell cultivations is demonstrated. The technology makes use of commercial printed circuit board (PCB) technology, integrated circuit sensors, and an electrochemical gas generation system. We present results for an array of eight 250 μl microbioreactors. Each bioreactor contains an independently addressable suite that provides closed‐loop temperature control, generates feed gas electrochemically, and continuously monitors optical density. The PCB technology allows for the assembly of additional off‐the‐shelf components into the microbioreactor array; we demonstrate the use of a commercial ISFET chip to continuously monitor culture pH. The electrochemical dosing system provides a powerful paradigm for reproducible gas delivery to high‐density arrays of microreactors. Growth data are presented for Escherichia coli cultured in the array with varying microaerobic conditions using electrochemically generated oxygen. 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Bioeng</addtitle><date>2004-02-20</date><risdate>2004</risdate><volume>85</volume><issue>4</issue><spage>376</spage><epage>381</epage><pages>376-381</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><abstract>A scalable array technology for parametric control of high‐throughput cell cultivations is demonstrated. The technology makes use of commercial printed circuit board (PCB) technology, integrated circuit sensors, and an electrochemical gas generation system. We present results for an array of eight 250 μl microbioreactors. Each bioreactor contains an independently addressable suite that provides closed‐loop temperature control, generates feed gas electrochemically, and continuously monitors optical density. The PCB technology allows for the assembly of additional off‐the‐shelf components into the microbioreactor array; we demonstrate the use of a commercial ISFET chip to continuously monitor culture pH. 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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Bioreactors - microbiology Cell Culture Techniques - instrumentation Cell Culture Techniques - methods electrochemical gas generation Electrochemistry - instrumentation Electrochemistry - methods Electrodes Equipment Design Equipment Failure Analysis Escherichia coli Escherichia coli - growth & development Escherichia coli - metabolism Feedback - physiology fermentation Hydrogen-Ion Concentration microbioreactor Miniaturization - methods Oxygen - metabolism parameter optimization Temperature |
| title | Microbioreactor arrays with parametric control for high-throughput experimentation |
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