Hantavirus strains isolated from rodentia and insectivora in rural China differentiated by polymerase chain reaction assay
Polymerase chain reaction (PCR) assay and other techniques were applied to differentiate Hantavirus strains isolated from different animal hosts and geographic regions in China. Two groups of related strains, Hantaan and Seoul, have been classified by cross-neutralization, radioimmunoprecipitation (...
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| Veröffentlicht in: | Archives of virology 1990, Vol.115 (1-2), p.37-46 |
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| container_title | Archives of virology |
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| creator | Tang, Y W Ruo, S L Xu, X Sanchez, A Fisher-Hoch, S P McCormick, J B Xu, Z Y |
| description | Polymerase chain reaction (PCR) assay and other techniques were applied to differentiate Hantavirus strains isolated from different animal hosts and geographic regions in China. Two groups of related strains, Hantaan and Seoul, have been classified by cross-neutralization, radioimmunoprecipitation (RIP), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assays. The molecular weights of glycoprotein 1 (G1) of Hantaan and Seoul viruses were 72k and 80k, whereas those of the nucleocapsid (N) and glycoprotein 2 (G2) remained the same, respectively. The PCR assay was used to differentiate these isolates using synthetic oligonucleotide primers selected from various regions of the M genome of 76118 and R22 strains. 76118-specific primers amplified only the RNAs extracted from Hantaan strains while R22-specific primers, the RNAs from Seoul strains. The PCR results for classification are consistent with those obtained by cross-neutralization, RIP and SDS-PAGE assays. |
| doi_str_mv | 10.1007/BF01310621 |
| format | Article |
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Two groups of related strains, Hantaan and Seoul, have been classified by cross-neutralization, radioimmunoprecipitation (RIP), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assays. The molecular weights of glycoprotein 1 (G1) of Hantaan and Seoul viruses were 72k and 80k, whereas those of the nucleocapsid (N) and glycoprotein 2 (G2) remained the same, respectively. The PCR assay was used to differentiate these isolates using synthetic oligonucleotide primers selected from various regions of the M genome of 76118 and R22 strains. 76118-specific primers amplified only the RNAs extracted from Hantaan strains while R22-specific primers, the RNAs from Seoul strains. The PCR results for classification are consistent with those obtained by cross-neutralization, RIP and SDS-PAGE assays.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/BF01310621</identifier><identifier>PMID: 2123383</identifier><language>eng</language><publisher>Austria</publisher><subject>Animals ; Base Sequence ; China ; Fluorescent Antibody Technique ; Gerbillinae - microbiology ; Hantavirus ; Hantavirus - classification ; Hantavirus - genetics ; Hantavirus - isolation & purification ; Molecular Sequence Data ; Muridae - microbiology ; Neutralization Tests ; Nucleic Acid Hybridization ; Polymerase Chain Reaction ; Radioimmunoprecipitation Assay ; Rats - microbiology ; RNA Probes ; RNA, Viral - analysis ; Shrews - microbiology ; Vero Cells ; Viral Proteins - analysis</subject><ispartof>Archives of virology, 1990, Vol.115 (1-2), p.37-46</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2641-d6ce27fb8c65fc9536ae987121e5c1dda5ead4a11c4bae73a2bdd9e0969591913</citedby><cites>FETCH-LOGICAL-c2641-d6ce27fb8c65fc9536ae987121e5c1dda5ead4a11c4bae73a2bdd9e0969591913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2123383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Y W</creatorcontrib><creatorcontrib>Ruo, S L</creatorcontrib><creatorcontrib>Xu, X</creatorcontrib><creatorcontrib>Sanchez, A</creatorcontrib><creatorcontrib>Fisher-Hoch, S P</creatorcontrib><creatorcontrib>McCormick, J B</creatorcontrib><creatorcontrib>Xu, Z Y</creatorcontrib><title>Hantavirus strains isolated from rodentia and insectivora in rural China differentiated by polymerase chain reaction assay</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>Polymerase chain reaction (PCR) assay and other techniques were applied to differentiate Hantavirus strains isolated from different animal hosts and geographic regions in China. Two groups of related strains, Hantaan and Seoul, have been classified by cross-neutralization, radioimmunoprecipitation (RIP), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assays. The molecular weights of glycoprotein 1 (G1) of Hantaan and Seoul viruses were 72k and 80k, whereas those of the nucleocapsid (N) and glycoprotein 2 (G2) remained the same, respectively. The PCR assay was used to differentiate these isolates using synthetic oligonucleotide primers selected from various regions of the M genome of 76118 and R22 strains. 76118-specific primers amplified only the RNAs extracted from Hantaan strains while R22-specific primers, the RNAs from Seoul strains. The PCR results for classification are consistent with those obtained by cross-neutralization, RIP and SDS-PAGE assays.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>China</subject><subject>Fluorescent Antibody Technique</subject><subject>Gerbillinae - microbiology</subject><subject>Hantavirus</subject><subject>Hantavirus - classification</subject><subject>Hantavirus - genetics</subject><subject>Hantavirus - isolation & purification</subject><subject>Molecular Sequence Data</subject><subject>Muridae - microbiology</subject><subject>Neutralization Tests</subject><subject>Nucleic Acid Hybridization</subject><subject>Polymerase Chain Reaction</subject><subject>Radioimmunoprecipitation Assay</subject><subject>Rats - microbiology</subject><subject>RNA Probes</subject><subject>RNA, Viral - analysis</subject><subject>Shrews - microbiology</subject><subject>Vero Cells</subject><subject>Viral Proteins - analysis</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1PwzAQxS0EKuVjYUfyxIAU8NmJk4xQ8SVVYoE5utgX1SiJi50glb-eFCoYme5093tveI-xMxBXIER-fXsvQIHQEvbYHFIlkyIvi302F0qkSaFFcciOYnwTYjqobMZmEqRShZqzz0fsB_xwYYw8DgFdH7mLvsWBLG-C73jwlvrBIcfe8ulNZnAfPuC08zAGbPli5Xrk1jUNhW90q603fO3bTUcBI3Gzwi1OOIl9zzFG3JywgwbbSKe7ecxe7-9eFo_J8vnhaXGzTIzUKSRWG5J5UxdGZ40pM6WRyiIHCZQZsBYzQpsigElrpFyhrK0tSZS6zEooQR2zix_fdfDvI8Wh6lw01LbYkx9jVUzh5VLLf0HQqVa52IKXP6AJPsZATbUOrsOwqUBU20aqv0Ym-HznOtYd2V90V4H6AskTiBw</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Tang, Y W</creator><creator>Ruo, S L</creator><creator>Xu, X</creator><creator>Sanchez, A</creator><creator>Fisher-Hoch, S P</creator><creator>McCormick, J B</creator><creator>Xu, Z Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Hantavirus strains isolated from rodentia and insectivora in rural China differentiated by polymerase chain reaction assay</title><author>Tang, Y W ; Ruo, S L ; Xu, X ; Sanchez, A ; Fisher-Hoch, S P ; McCormick, J B ; Xu, Z Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2641-d6ce27fb8c65fc9536ae987121e5c1dda5ead4a11c4bae73a2bdd9e0969591913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>China</topic><topic>Fluorescent Antibody Technique</topic><topic>Gerbillinae - microbiology</topic><topic>Hantavirus</topic><topic>Hantavirus - classification</topic><topic>Hantavirus - genetics</topic><topic>Hantavirus - isolation & purification</topic><topic>Molecular Sequence Data</topic><topic>Muridae - microbiology</topic><topic>Neutralization Tests</topic><topic>Nucleic Acid Hybridization</topic><topic>Polymerase Chain Reaction</topic><topic>Radioimmunoprecipitation Assay</topic><topic>Rats - microbiology</topic><topic>RNA Probes</topic><topic>RNA, Viral - analysis</topic><topic>Shrews - microbiology</topic><topic>Vero Cells</topic><topic>Viral Proteins - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Y W</creatorcontrib><creatorcontrib>Ruo, S L</creatorcontrib><creatorcontrib>Xu, X</creatorcontrib><creatorcontrib>Sanchez, A</creatorcontrib><creatorcontrib>Fisher-Hoch, S P</creatorcontrib><creatorcontrib>McCormick, J B</creatorcontrib><creatorcontrib>Xu, Z Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Y W</au><au>Ruo, S L</au><au>Xu, X</au><au>Sanchez, A</au><au>Fisher-Hoch, S P</au><au>McCormick, J B</au><au>Xu, Z Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hantavirus strains isolated from rodentia and insectivora in rural China differentiated by polymerase chain reaction assay</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1990</date><risdate>1990</risdate><volume>115</volume><issue>1-2</issue><spage>37</spage><epage>46</epage><pages>37-46</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Polymerase chain reaction (PCR) assay and other techniques were applied to differentiate Hantavirus strains isolated from different animal hosts and geographic regions in China. Two groups of related strains, Hantaan and Seoul, have been classified by cross-neutralization, radioimmunoprecipitation (RIP), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assays. The molecular weights of glycoprotein 1 (G1) of Hantaan and Seoul viruses were 72k and 80k, whereas those of the nucleocapsid (N) and glycoprotein 2 (G2) remained the same, respectively. The PCR assay was used to differentiate these isolates using synthetic oligonucleotide primers selected from various regions of the M genome of 76118 and R22 strains. 76118-specific primers amplified only the RNAs extracted from Hantaan strains while R22-specific primers, the RNAs from Seoul strains. The PCR results for classification are consistent with those obtained by cross-neutralization, RIP and SDS-PAGE assays.</abstract><cop>Austria</cop><pmid>2123383</pmid><doi>10.1007/BF01310621</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-8608 |
| ispartof | Archives of virology, 1990, Vol.115 (1-2), p.37-46 |
| issn | 0304-8608 1432-8798 |
| language | eng |
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| source | MEDLINE; Springer Online Journals Complete |
| subjects | Animals Base Sequence China Fluorescent Antibody Technique Gerbillinae - microbiology Hantavirus Hantavirus - classification Hantavirus - genetics Hantavirus - isolation & purification Molecular Sequence Data Muridae - microbiology Neutralization Tests Nucleic Acid Hybridization Polymerase Chain Reaction Radioimmunoprecipitation Assay Rats - microbiology RNA Probes RNA, Viral - analysis Shrews - microbiology Vero Cells Viral Proteins - analysis |
| title | Hantavirus strains isolated from rodentia and insectivora in rural China differentiated by polymerase chain reaction assay |
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