Comparative cytokine profile of human skin mast cells from two compartments—strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming

Although known as heterogenous, mast cells (MC) are believed to induce allergic inflammation, partially by secretion of T helper cell type 2 (Th2) cytokines. We show here that MC purified from twohuman skin compartments produce cytokines that are primarily associated with inflammation and innate imm...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of leukocyte biology 2004-02, Vol.75 (2), p.244-252
Hauptverfasser: Babina, Magda, Guhl, Sven, Stärke, André, Kirchhof, Loreen, Zuberbier, Torsten, Henz, Beate M.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 252
container_issue 2
container_start_page 244
container_title Journal of leukocyte biology
container_volume 75
creator Babina, Magda
Guhl, Sven
Stärke, André
Kirchhof, Loreen
Zuberbier, Torsten
Henz, Beate M.
description Although known as heterogenous, mast cells (MC) are believed to induce allergic inflammation, partially by secretion of T helper cell type 2 (Th2) cytokines. We show here that MC purified from twohuman skin compartments produce cytokines that are primarily associated with inflammation and innate immunity [interleukin (IL)‐1β, IL‐6, IL‐8, tumor necrosis factor α (TNF‐α)]. Although these are detectable even without stimulation, immunoglobulin (Ig)E receptor cross‐linking is able to enhance only TNF‐α production, but phorbol 12‐myristate 13‐acetate additionally promotes IL‐1β and IL‐8. With the exception of TNF‐α, the presence of serum has a positive impact on cytokine production. Although IL‐13 transcripts (but not those for IL‐4 and ‐5) are produced by skin MC, all Th2 cytokines remain undetectable in the supernatants or lysates of MC from foreskin and breast skin by all treatments. Therefore, rather than sharing similarity with Th2 cells, the cytokine profile of skin MC at baseline resembles that of monocytes. Of note, MC precultured in the presence of IL‐4 [alone or plus stem cell factor (SCF)] before anti‐IgE stimulation, acquired the ability to produce IL‐5, and IL‐1β was concomitantly suppressed. Additionally, strong up‐regulation of IL‐6 by SCF was observed, which was inhibited by IL‐4. In summary, we present a detailed analysis of the cytokine array of human skin MC immediately upon isolation; demonstrate that MC from different skin compartments, although producing the same pattern of cytokines, display quantitative differences in several aspects; and provide further evidence that MC possess a proinflammatory capacity, which can, however, be altered by microenvironmental stimuli, substantiating the marked plasticity of the cells.
doi_str_mv 10.1189/jlb.0403157
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80136914</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80136914</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4244-2b3348aa6f5a859940781a38c1138a94648a12de9bd9d0d4ce13e2f738ebc3893</originalsourceid><addsrcrecordid>eNqFkc2OFCEUhYnROO3oyr1hoxtTIxRUFSydjj9jOnGja0JRt7oZCxiBstI7H8IX8NV8Eim7E3e6ugl851wOB6GnlFxRKuSr26m_Ipww2nT30IZKJirWduw-2pCO06rhhFygRyndEkJY3ZKH6ILylnHSNhv0cxvcnY4622-AzTGHL9YDvothtBPgMOLD7LTHqRxjp1PGBqYp4TEGh_MSsPkjzw58Tr--_0g5Br_HERK4ftLeAF5sPmAXfCjukLDOuNcJpnVNP2ds_TCbbINfl93sqgb3x3Xy8gjrrN8_Rg9GPSV4cp6X6PPbN5-276vdx3c329e7yvCa86ruGeNC63ZstGik5KQTVDNhKGVCS96WS1oPIPtBDmTgBiiDeuyYgN4wIdklenHyLeG_zpCycjatabWHMCclCGWtpPy_IJV1R6hcwZcn0MSQUoRRrZF0PCpK1FqdKtWpc3WFfna2nXsHw1_23FUByAlYSjXHf3mpD7trUj6lSJ6fJAe7Pyw2gkpOT1PZUKtlWbpG1WrlfgPDpLNK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19270194</pqid></control><display><type>article</type><title>Comparative cytokine profile of human skin mast cells from two compartments—strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming</title><source>Wiley-Blackwell Journals</source><source>MEDLINE</source><source>Oxford Academic Journals (OUP)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Babina, Magda ; Guhl, Sven ; Stärke, André ; Kirchhof, Loreen ; Zuberbier, Torsten ; Henz, Beate M.</creator><creatorcontrib>Babina, Magda ; Guhl, Sven ; Stärke, André ; Kirchhof, Loreen ; Zuberbier, Torsten ; Henz, Beate M.</creatorcontrib><description>Although known as heterogenous, mast cells (MC) are believed to induce allergic inflammation, partially by secretion of T helper cell type 2 (Th2) cytokines. We show here that MC purified from twohuman skin compartments produce cytokines that are primarily associated with inflammation and innate immunity [interleukin (IL)‐1β, IL‐6, IL‐8, tumor necrosis factor α (TNF‐α)]. Although these are detectable even without stimulation, immunoglobulin (Ig)E receptor cross‐linking is able to enhance only TNF‐α production, but phorbol 12‐myristate 13‐acetate additionally promotes IL‐1β and IL‐8. With the exception of TNF‐α, the presence of serum has a positive impact on cytokine production. Although IL‐13 transcripts (but not those for IL‐4 and ‐5) are produced by skin MC, all Th2 cytokines remain undetectable in the supernatants or lysates of MC from foreskin and breast skin by all treatments. Therefore, rather than sharing similarity with Th2 cells, the cytokine profile of skin MC at baseline resembles that of monocytes. Of note, MC precultured in the presence of IL‐4 [alone or plus stem cell factor (SCF)] before anti‐IgE stimulation, acquired the ability to produce IL‐5, and IL‐1β was concomitantly suppressed. Additionally, strong up‐regulation of IL‐6 by SCF was observed, which was inhibited by IL‐4. In summary, we present a detailed analysis of the cytokine array of human skin MC immediately upon isolation; demonstrate that MC from different skin compartments, although producing the same pattern of cytokines, display quantitative differences in several aspects; and provide further evidence that MC possess a proinflammatory capacity, which can, however, be altered by microenvironmental stimuli, substantiating the marked plasticity of the cells.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1189/jlb.0403157</identifier><identifier>PMID: 14634065</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>cell plasticity ; Culture Media - pharmacology ; Cytokines - biosynthesis ; Gene Expression Regulation - drug effects ; Humans ; Inflammation ; innate immunity ; interleukin ; Interleukin-4 - pharmacology ; Interleukin-5 - biosynthesis ; Mast Cells - immunology ; Mast Cells - metabolism ; Monocytes ; Skin - cytology ; Th2 Cells - immunology</subject><ispartof>Journal of leukocyte biology, 2004-02, Vol.75 (2), p.244-252</ispartof><rights>2004 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4244-2b3348aa6f5a859940781a38c1138a94648a12de9bd9d0d4ce13e2f738ebc3893</citedby><cites>FETCH-LOGICAL-c4244-2b3348aa6f5a859940781a38c1138a94648a12de9bd9d0d4ce13e2f738ebc3893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1189%2Fjlb.0403157$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1189%2Fjlb.0403157$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14634065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Babina, Magda</creatorcontrib><creatorcontrib>Guhl, Sven</creatorcontrib><creatorcontrib>Stärke, André</creatorcontrib><creatorcontrib>Kirchhof, Loreen</creatorcontrib><creatorcontrib>Zuberbier, Torsten</creatorcontrib><creatorcontrib>Henz, Beate M.</creatorcontrib><title>Comparative cytokine profile of human skin mast cells from two compartments—strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>Although known as heterogenous, mast cells (MC) are believed to induce allergic inflammation, partially by secretion of T helper cell type 2 (Th2) cytokines. We show here that MC purified from twohuman skin compartments produce cytokines that are primarily associated with inflammation and innate immunity [interleukin (IL)‐1β, IL‐6, IL‐8, tumor necrosis factor α (TNF‐α)]. Although these are detectable even without stimulation, immunoglobulin (Ig)E receptor cross‐linking is able to enhance only TNF‐α production, but phorbol 12‐myristate 13‐acetate additionally promotes IL‐1β and IL‐8. With the exception of TNF‐α, the presence of serum has a positive impact on cytokine production. Although IL‐13 transcripts (but not those for IL‐4 and ‐5) are produced by skin MC, all Th2 cytokines remain undetectable in the supernatants or lysates of MC from foreskin and breast skin by all treatments. Therefore, rather than sharing similarity with Th2 cells, the cytokine profile of skin MC at baseline resembles that of monocytes. Of note, MC precultured in the presence of IL‐4 [alone or plus stem cell factor (SCF)] before anti‐IgE stimulation, acquired the ability to produce IL‐5, and IL‐1β was concomitantly suppressed. Additionally, strong up‐regulation of IL‐6 by SCF was observed, which was inhibited by IL‐4. In summary, we present a detailed analysis of the cytokine array of human skin MC immediately upon isolation; demonstrate that MC from different skin compartments, although producing the same pattern of cytokines, display quantitative differences in several aspects; and provide further evidence that MC possess a proinflammatory capacity, which can, however, be altered by microenvironmental stimuli, substantiating the marked plasticity of the cells.</description><subject>cell plasticity</subject><subject>Culture Media - pharmacology</subject><subject>Cytokines - biosynthesis</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Inflammation</subject><subject>innate immunity</subject><subject>interleukin</subject><subject>Interleukin-4 - pharmacology</subject><subject>Interleukin-5 - biosynthesis</subject><subject>Mast Cells - immunology</subject><subject>Mast Cells - metabolism</subject><subject>Monocytes</subject><subject>Skin - cytology</subject><subject>Th2 Cells - immunology</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OFCEUhYnROO3oyr1hoxtTIxRUFSydjj9jOnGja0JRt7oZCxiBstI7H8IX8NV8Eim7E3e6ugl851wOB6GnlFxRKuSr26m_Ipww2nT30IZKJirWduw-2pCO06rhhFygRyndEkJY3ZKH6ILylnHSNhv0cxvcnY4622-AzTGHL9YDvothtBPgMOLD7LTHqRxjp1PGBqYp4TEGh_MSsPkjzw58Tr--_0g5Br_HERK4ftLeAF5sPmAXfCjukLDOuNcJpnVNP2ds_TCbbINfl93sqgb3x3Xy8gjrrN8_Rg9GPSV4cp6X6PPbN5-276vdx3c329e7yvCa86ruGeNC63ZstGik5KQTVDNhKGVCS96WS1oPIPtBDmTgBiiDeuyYgN4wIdklenHyLeG_zpCycjatabWHMCclCGWtpPy_IJV1R6hcwZcn0MSQUoRRrZF0PCpK1FqdKtWpc3WFfna2nXsHw1_23FUByAlYSjXHf3mpD7trUj6lSJ6fJAe7Pyw2gkpOT1PZUKtlWbpG1WrlfgPDpLNK</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Babina, Magda</creator><creator>Guhl, Sven</creator><creator>Stärke, André</creator><creator>Kirchhof, Loreen</creator><creator>Zuberbier, Torsten</creator><creator>Henz, Beate M.</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040201</creationdate><title>Comparative cytokine profile of human skin mast cells from two compartments—strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming</title><author>Babina, Magda ; Guhl, Sven ; Stärke, André ; Kirchhof, Loreen ; Zuberbier, Torsten ; Henz, Beate M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4244-2b3348aa6f5a859940781a38c1138a94648a12de9bd9d0d4ce13e2f738ebc3893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>cell plasticity</topic><topic>Culture Media - pharmacology</topic><topic>Cytokines - biosynthesis</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Inflammation</topic><topic>innate immunity</topic><topic>interleukin</topic><topic>Interleukin-4 - pharmacology</topic><topic>Interleukin-5 - biosynthesis</topic><topic>Mast Cells - immunology</topic><topic>Mast Cells - metabolism</topic><topic>Monocytes</topic><topic>Skin - cytology</topic><topic>Th2 Cells - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Babina, Magda</creatorcontrib><creatorcontrib>Guhl, Sven</creatorcontrib><creatorcontrib>Stärke, André</creatorcontrib><creatorcontrib>Kirchhof, Loreen</creatorcontrib><creatorcontrib>Zuberbier, Torsten</creatorcontrib><creatorcontrib>Henz, Beate M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Babina, Magda</au><au>Guhl, Sven</au><au>Stärke, André</au><au>Kirchhof, Loreen</au><au>Zuberbier, Torsten</au><au>Henz, Beate M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative cytokine profile of human skin mast cells from two compartments—strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2004-02-01</date><risdate>2004</risdate><volume>75</volume><issue>2</issue><spage>244</spage><epage>252</epage><pages>244-252</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>Although known as heterogenous, mast cells (MC) are believed to induce allergic inflammation, partially by secretion of T helper cell type 2 (Th2) cytokines. We show here that MC purified from twohuman skin compartments produce cytokines that are primarily associated with inflammation and innate immunity [interleukin (IL)‐1β, IL‐6, IL‐8, tumor necrosis factor α (TNF‐α)]. Although these are detectable even without stimulation, immunoglobulin (Ig)E receptor cross‐linking is able to enhance only TNF‐α production, but phorbol 12‐myristate 13‐acetate additionally promotes IL‐1β and IL‐8. With the exception of TNF‐α, the presence of serum has a positive impact on cytokine production. Although IL‐13 transcripts (but not those for IL‐4 and ‐5) are produced by skin MC, all Th2 cytokines remain undetectable in the supernatants or lysates of MC from foreskin and breast skin by all treatments. Therefore, rather than sharing similarity with Th2 cells, the cytokine profile of skin MC at baseline resembles that of monocytes. Of note, MC precultured in the presence of IL‐4 [alone or plus stem cell factor (SCF)] before anti‐IgE stimulation, acquired the ability to produce IL‐5, and IL‐1β was concomitantly suppressed. Additionally, strong up‐regulation of IL‐6 by SCF was observed, which was inhibited by IL‐4. In summary, we present a detailed analysis of the cytokine array of human skin MC immediately upon isolation; demonstrate that MC from different skin compartments, although producing the same pattern of cytokines, display quantitative differences in several aspects; and provide further evidence that MC possess a proinflammatory capacity, which can, however, be altered by microenvironmental stimuli, substantiating the marked plasticity of the cells.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>14634065</pmid><doi>10.1189/jlb.0403157</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0741-5400
ispartof Journal of leukocyte biology, 2004-02, Vol.75 (2), p.244-252
issn 0741-5400
1938-3673
language eng
recordid cdi_proquest_miscellaneous_80136914
source Wiley-Blackwell Journals; MEDLINE; Oxford Academic Journals (OUP); EZB-FREE-00999 freely available EZB journals
subjects cell plasticity
Culture Media - pharmacology
Cytokines - biosynthesis
Gene Expression Regulation - drug effects
Humans
Inflammation
innate immunity
interleukin
Interleukin-4 - pharmacology
Interleukin-5 - biosynthesis
Mast Cells - immunology
Mast Cells - metabolism
Monocytes
Skin - cytology
Th2 Cells - immunology
title Comparative cytokine profile of human skin mast cells from two compartments—strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T11%3A07%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20cytokine%20profile%20of%20human%20skin%20mast%20cells%20from%20two%20compartments%E2%80%94strong%20resemblance%20with%20monocytes%20at%20baseline%20but%20induction%20of%20IL-5%20by%20IL-4%20priming&rft.jtitle=Journal%20of%20leukocyte%20biology&rft.au=Babina,%20Magda&rft.date=2004-02-01&rft.volume=75&rft.issue=2&rft.spage=244&rft.epage=252&rft.pages=244-252&rft.issn=0741-5400&rft.eissn=1938-3673&rft_id=info:doi/10.1189/jlb.0403157&rft_dat=%3Cproquest_cross%3E80136914%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19270194&rft_id=info:pmid/14634065&rfr_iscdi=true