A Microphysiometer for Simultaneous Measurement of Changes in Extracellular Glucose, Lactate, Oxygen, and Acidification Rate

A microphysiometer capable of measuring changes in extracellular glucose, lactate, oxygen, and acidification rate has been developed by incorporating modified electrodes into a standard Cytosensor Microphysiometer plunger. Glucose and lactate are measured indirectly at platinum electrodes by amperom...

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Veröffentlicht in:	Analytical chemistry (Washington) 2004-02, Vol.76 (3), p.519-527
Hauptverfasser:	Eklund, Sven E, Taylor, Dale, Kozlov, Eugene, Prokop, Ales, Cliffel, David E
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Biosensing Techniques - instrumentation Biosensing Techniques - standards Cells Chemistry CHO Cells Cricetinae Electrochemical methods Electrodes Enzymes Exact sciences and technology Extracellular Space - metabolism Female Fibroblasts - metabolism Glucose - analysis Hydrogen-Ion Concentration Instruments Lactic Acid - analysis Measurement Mice Oxygen Oxygen - analysis Platinum - chemistry Rodents Sensitivity and Specificity
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