Analysis of a Human Fungiform Papillae cDNA Library and Identification of Taste-related Genes
Various genes related to early events in human gustation have recently been discovered, yet a thorough understanding of taste transduction is hampered by gaps in our knowledge of the signaling chain. As a first step toward gaining additional insight, the expression specificity of genes in human tast...
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| Veröffentlicht in: | Chemical senses 2004-01, Vol.29 (1), p.13-23 |
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| creator | Rossier, Olivier Cao, Jie Huque, Taufiqul Spielman, Andrew I. Feldman, Roy S. Medrano, Juan F. Brand, Joseph G. le Coutre, Johannes |
| description | Various genes related to early events in human gustation have recently been discovered, yet a thorough understanding of taste transduction is hampered by gaps in our knowledge of the signaling chain. As a first step toward gaining additional insight, the expression specificity of genes in human taste tissue needs to be determined. To this end, a fungiform papillae cDNA library has been generated and analyzed. For validation of the library, taste-related gene probes were used to detect known molecules. Subsequently, DNA sequence analysis was performed to identify further candidates. Of 987 clones sequenced, clustering results in 288 contigs. Comparison of these contigs with genomic databases reveals that 207 contigs (71.9%) match known genes, 16 (5.6%) match hypothetical genes, eight (2.8%) match repetitive sequences and 57 (19.8%) have no or low similarity to annotated genes. The results indicate that despite a high level of redundancy, this human fungiform cDNA library contains specific taste markers and is valuable for investigation of both known and novel taste-related genes. |
| doi_str_mv | 10.1093/chemse/bjh002 |
| format | Article |
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As a first step toward gaining additional insight, the expression specificity of genes in human taste tissue needs to be determined. To this end, a fungiform papillae cDNA library has been generated and analyzed. For validation of the library, taste-related gene probes were used to detect known molecules. Subsequently, DNA sequence analysis was performed to identify further candidates. Of 987 clones sequenced, clustering results in 288 contigs. Comparison of these contigs with genomic databases reveals that 207 contigs (71.9%) match known genes, 16 (5.6%) match hypothetical genes, eight (2.8%) match repetitive sequences and 57 (19.8%) have no or low similarity to annotated genes. The results indicate that despite a high level of redundancy, this human fungiform cDNA library contains specific taste markers and is valuable for investigation of both known and novel taste-related genes.</description><identifier>ISSN: 0379-864X</identifier><identifier>ISSN: 1464-3553</identifier><identifier>EISSN: 1464-3553</identifier><identifier>DOI: 10.1093/chemse/bjh002</identifier><identifier>PMID: 14752036</identifier><identifier>CODEN: CHSED8</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; cDNA library ; Computational Biology ; Contig Mapping ; Databases, Nucleic Acid ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Library ; human fungiform papillae ; Humans ; Olfaction. Taste ; Perception ; Proteins - classification ; Proteins - genetics ; Psychology. Psychoanalysis. Psychiatry ; Psychology. Psychophysiology ; Sequence Analysis ; signal transduction ; Signal Transduction - genetics ; Taste - genetics ; Taste Buds ; taste marker ; tastomics</subject><ispartof>Chemical senses, 2004-01, Vol.29 (1), p.13-23</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Jan 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-f388160e5436c1fea8addd7bae6686bc8c2fc8f978648fc55b9ef21f3f9d6a2e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27902,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15541273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14752036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rossier, Olivier</creatorcontrib><creatorcontrib>Cao, Jie</creatorcontrib><creatorcontrib>Huque, Taufiqul</creatorcontrib><creatorcontrib>Spielman, Andrew I.</creatorcontrib><creatorcontrib>Feldman, Roy S.</creatorcontrib><creatorcontrib>Medrano, Juan F.</creatorcontrib><creatorcontrib>Brand, Joseph G.</creatorcontrib><creatorcontrib>le Coutre, Johannes</creatorcontrib><title>Analysis of a Human Fungiform Papillae cDNA Library and Identification of Taste-related Genes</title><title>Chemical senses</title><addtitle>Chem. Senses</addtitle><description>Various genes related to early events in human gustation have recently been discovered, yet a thorough understanding of taste transduction is hampered by gaps in our knowledge of the signaling chain. As a first step toward gaining additional insight, the expression specificity of genes in human taste tissue needs to be determined. To this end, a fungiform papillae cDNA library has been generated and analyzed. For validation of the library, taste-related gene probes were used to detect known molecules. Subsequently, DNA sequence analysis was performed to identify further candidates. Of 987 clones sequenced, clustering results in 288 contigs. Comparison of these contigs with genomic databases reveals that 207 contigs (71.9%) match known genes, 16 (5.6%) match hypothetical genes, eight (2.8%) match repetitive sequences and 57 (19.8%) have no or low similarity to annotated genes. The results indicate that despite a high level of redundancy, this human fungiform cDNA library contains specific taste markers and is valuable for investigation of both known and novel taste-related genes.</description><subject>Biological and medical sciences</subject><subject>cDNA library</subject><subject>Computational Biology</subject><subject>Contig Mapping</subject><subject>Databases, Nucleic Acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Library</subject><subject>human fungiform papillae</subject><subject>Humans</subject><subject>Olfaction. Taste</subject><subject>Perception</subject><subject>Proteins - classification</subject><subject>Proteins - genetics</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychology. Psychophysiology</subject><subject>Sequence Analysis</subject><subject>signal transduction</subject><subject>Signal Transduction - genetics</subject><subject>Taste - genetics</subject><subject>Taste Buds</subject><subject>taste marker</subject><subject>tastomics</subject><issn>0379-864X</issn><issn>1464-3553</issn><issn>1464-3553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1rFDEYBvAgFrtWj14lCPY2Nt-TOa5r2y0s1cOqRZCQybyxWedjTWbA_vdm2aULXnrKIb8kT94HoTeUfKCk4hfuHroEF_XmnhD2DM2oUKLgUvLnaEZ4WRVaibtT9DKlDSFUcKZfoFMqSskIVzP0c97b9iGFhAePLV5One3x1dT_Cn6IHf5it6FtLWD36XaOV6GONj5g2zf4poF-DD44O4ah351e2zRCEaG1IzT4GnpIr9CJt22C14f1DH29ulwvlsXq8_XNYr4qXI4xFp5rTRUBKbhy1IPVtmmasraglFa10455p31V5r9o76SsK_CMeu6rRlkG_Ayd7-_dxuHPBGk0XUgOcvIehikZTSgXhMknIa1ykhwlw3f_wc0wxTyrnalYyZQWGRV75OKQUgRvtjF0eUKGErNrx-zbMft2sn97uHSqO2iO-lBHBu8PwCZnWx9t70I6OikFZSU_PhzyyP8-7tv426iSl9Is734Yvvi4_qb5dyP4P6MYqGE</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Rossier, Olivier</creator><creator>Cao, Jie</creator><creator>Huque, Taufiqul</creator><creator>Spielman, Andrew I.</creator><creator>Feldman, Roy S.</creator><creator>Medrano, Juan F.</creator><creator>Brand, Joseph G.</creator><creator>le Coutre, Johannes</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200401</creationdate><title>Analysis of a Human Fungiform Papillae cDNA Library and Identification of Taste-related Genes</title><author>Rossier, Olivier ; Cao, Jie ; Huque, Taufiqul ; Spielman, Andrew I. ; Feldman, Roy S. ; Medrano, Juan F. ; Brand, Joseph G. ; le Coutre, Johannes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-f388160e5436c1fea8addd7bae6686bc8c2fc8f978648fc55b9ef21f3f9d6a2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>cDNA library</topic><topic>Computational Biology</topic><topic>Contig Mapping</topic><topic>Databases, Nucleic Acid</topic><topic>Fundamental and applied biological sciences. 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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Biological and medical sciences cDNA library Computational Biology Contig Mapping Databases, Nucleic Acid Fundamental and applied biological sciences. Psychology gene expression Gene Library human fungiform papillae Humans Olfaction. Taste Perception Proteins - classification Proteins - genetics Psychology. Psychoanalysis. Psychiatry Psychology. Psychophysiology Sequence Analysis signal transduction Signal Transduction - genetics Taste - genetics Taste Buds taste marker tastomics |
| title | Analysis of a Human Fungiform Papillae cDNA Library and Identification of Taste-related Genes |
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