Multiplexed Toxin Analysis Using Four Colors of Quantum Dot Fluororeagents
Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. In this work, we prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals (CdSe−ZnS core−shell QDs) and antibodies to perform multiplexed fluoroimmunoassays. Sandwich immunoassa...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2004-02, Vol.76 (3), p.684-688 |
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| creator | Goldman, Ellen R Clapp, Aaron R Anderson, George P Uyeda, H. Tetsuo Mauro, J. Matthew Medintz, Igor L Mattoussi, Hedi |
| description | Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. In this work, we prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals (CdSe−ZnS core−shell QDs) and antibodies to perform multiplexed fluoroimmunoassays. Sandwich immunoassays for the detection of cholera toxin, ricin, shiga-like toxin 1, and staphylococcal enterotoxin B were performed simultaneously in single wells of a microtiter plate. Initially the assay performance for the detection of each toxin was examined. We then demonstrated the simultaneous detection of the four toxins from a single sample probed with a mixture of all four QD−antibody reagents. Using a simple linear equation-based algorithm, it was possible to deconvolute the signal from mixed toxin samples, which allowed quantitation of all four toxins simultaneously. |
| doi_str_mv | 10.1021/ac035083r |
| format | Article |
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Matthew ; Medintz, Igor L ; Mattoussi, Hedi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a472t-566cfe556fcc1750aaa59bcf4ac138d57608826623ee6b0f6d73f8fb2ef14a143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Algorithms</topic><topic>Analytical chemistry</topic><topic>Cadmium - chemistry</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Fluorescent Antibody Technique</topic><topic>Fluorometry - methods</topic><topic>Immunoconjugates - chemistry</topic><topic>Immunoenzyme Techniques</topic><topic>Instruments</topic><topic>Luminescent Measurements</topic><topic>Miscellaneous</topic><topic>Models, Biological</topic><topic>Nanocrystals</topic><topic>Protein Engineering - methods</topic><topic>Quantum Dots</topic><topic>Selenium - chemistry</topic><topic>Semiconductors</topic><topic>Sulfides - chemistry</topic><topic>Toxins</topic><topic>Toxins, Biological - analysis</topic><topic>Toxins, Biological - chemistry</topic><topic>Zinc Compounds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goldman, Ellen R</creatorcontrib><creatorcontrib>Clapp, Aaron R</creatorcontrib><creatorcontrib>Anderson, George P</creatorcontrib><creatorcontrib>Uyeda, H. 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| subjects | Algorithms Analytical chemistry Cadmium - chemistry Chemistry Exact sciences and technology Fluorescent Antibody Technique Fluorometry - methods Immunoconjugates - chemistry Immunoenzyme Techniques Instruments Luminescent Measurements Miscellaneous Models, Biological Nanocrystals Protein Engineering - methods Quantum Dots Selenium - chemistry Semiconductors Sulfides - chemistry Toxins Toxins, Biological - analysis Toxins, Biological - chemistry Zinc Compounds - chemistry |
| title | Multiplexed Toxin Analysis Using Four Colors of Quantum Dot Fluororeagents |
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