Removal of twelve C-terminal amino acids from firefly luciferase abolishes activity

cDNA coding for the luciferase in the firefly Photinus pyralis was cloned using pcDV1 primer and Honjo linker containing SP6 RNA polymerase promoter. This enabled conditions to be established to produce mRNA, capped with m 7 GpppG, in vitro and then translated to form light emitting protein. Full le...

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Veröffentlicht in:	Biochemical and biophysical research communications 1990-01, Vol.172 (2), p.477-482
Hauptverfasser:	Sala-Newby, Graciela, Kalsheker, Noor, Campbell, Anthony K.
Format:	Artikel
Sprache:	eng
Schlagworte:	acide amine actividad enzimatica activite enzymatique adn amino acids aminoacidos Analytical, structural and metabolic biochemistry Animals Base Sequence Biological and medical sciences clone clones Cloning, Molecular coleoptera Coleoptera - enzymology Coleoptera - genetics dna enzimas enzyme enzymes Enzymes and enzyme inhibitors enzymic activity fisiologia vegetal Fundamental and applied biological sciences. Psychology Genetic Variation luciferase Luciferases - genetics Luciferases - metabolism Molecular Sequence Data nucleotide nucleotides nucleotidos Oligonucleotide Probes Oxidoreductases physiologie vegetale plant physiology Polymerase Chain Reaction Promoter Regions, Genetic Protein Biosynthesis Transcription, Genetic transferasas transferase transferases
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