Evaluation of a new NASBA assay for the qualitative detection of hepatitis C virus based on the NucliSens® Basic Kit reagents

Background: Direct detection of HCV RNA by nucleic acid amplification methods is an essential tool in the diagnosis of HCV infections. In-house developed methods based on reverse transcribed polymerase chain reaction (RT-PCR) are widely used but they are laborious and usually lack the standardizatio...

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Veröffentlicht in:	Journal of clinical virology 2004-02, Vol.29 (2), p.84-91
Hauptverfasser:	Guichón, Andrea, Chiparelli, Héctor, Martı́nez, Alfredo, Rodrı́guez, Claudia, Trento, Alfonsina, Russi, José C, Carballal, Guadalupe
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Clinical Laboratory Techniques - methods DNA Fingerprinting Fundamental and applied biological sciences. Psychology Genotype HCV Hepacivirus - genetics Hepacivirus - isolation & purification Hepatitis C - diagnosis Hepatitis C - virology Hepatitis C virus Human viral diseases Humans Infectious diseases Medical sciences Microbiology Miscellaneous Molecular diagnosis NASBA Nucleic Acid Hybridization - methods NucliSens® Basic Kit Plasma - virology Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - blood RT-PCR Self-Sustained Sequence Replication - methods Sensitivity and Specificity Viral diseases Virology
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