Type-specific enzyme immunoassay for detection of pneumococcal capsular polysaccharide antigens in nasopharyngeal specimens
We developed a new competitive EIA method for the demonstration of pneumococcal capsular polysaccharides from respiratory samples. The pediatric types 4, 6B, 9V, 14, 18C, 19F and 23F were selected for this study, because these capsular polysaccharides were included in the first heptavalent pneumococ...
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| Veröffentlicht in: | Journal of microbiological methods 2004-02, Vol.56 (2), p.193-199 |
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| container_title | Journal of microbiological methods |
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| creator | Lankinen, K.S. Rintamäki, S. Syrjänen, R. Kilpi, T. Ruutu, P. Leinonen, M. |
| description | We developed a new competitive EIA method for the demonstration of pneumococcal capsular polysaccharides from respiratory samples. The pediatric types 4, 6B, 9V, 14, 18C, 19F and 23F were selected for this study, because these capsular polysaccharides were included in the first heptavalent pneumococcal conjugate vaccines, which were used in the Finnish Otitis Media Vaccine Trial. Sensitivity of the EIA tests for purified polysaccharide antigens varied between 5 and 100 ng/ml, depending on the type. The assays performed well in 100 nasopharyngeal samples (NPS) samples processed through an enrichment culture, with an almost 100% sensitivity compared with routine culture. The method appeared type-specific, except that EIA for 6B capsule also detected 6A. The method is applicable for type-specific identification of pneumococcus in carriage studies. |
| doi_str_mv | 10.1016/j.mimet.2003.10.021 |
| format | Article |
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The pediatric types 4, 6B, 9V, 14, 18C, 19F and 23F were selected for this study, because these capsular polysaccharides were included in the first heptavalent pneumococcal conjugate vaccines, which were used in the Finnish Otitis Media Vaccine Trial. Sensitivity of the EIA tests for purified polysaccharide antigens varied between 5 and 100 ng/ml, depending on the type. The assays performed well in 100 nasopharyngeal samples (NPS) samples processed through an enrichment culture, with an almost 100% sensitivity compared with routine culture. The method appeared type-specific, except that EIA for 6B capsule also detected 6A. The method is applicable for type-specific identification of pneumococcus in carriage studies.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2003.10.021</identifier><identifier>PMID: 14744448</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Antibodies, Bacterial - immunology ; Antigens, Bacterial - analysis ; Antigens, Bacterial - immunology ; Bacterial Capsules - analysis ; Bacterial Capsules - immunology ; Bacteriology ; Biological and medical sciences ; Capsular polysaccharide antigens ; Cohort Studies ; Enzyme immunoassay ; Finland ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoenzyme Techniques - methods ; Infant ; Microbiology ; Miscellaneous ; Nasopharyngeal specimens ; Nasopharynx - microbiology ; Pneumococcal Infections - microbiology ; Sensitivity and Specificity ; Streptococcus pneumoniae ; Streptococcus pneumoniae - immunology ; Streptococcus pneumoniae - isolation & purification</subject><ispartof>Journal of microbiological methods, 2004-02, Vol.56 (2), p.193-199</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-e12c49942557e4bc49bc6ec230c628abd1a0603b2cbe0aa91db3d0c54989803f3</citedby><cites>FETCH-LOGICAL-c416t-e12c49942557e4bc49bc6ec230c628abd1a0603b2cbe0aa91db3d0c54989803f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2003.10.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15477879$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14744448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lankinen, K.S.</creatorcontrib><creatorcontrib>Rintamäki, S.</creatorcontrib><creatorcontrib>Syrjänen, R.</creatorcontrib><creatorcontrib>Kilpi, T.</creatorcontrib><creatorcontrib>Ruutu, P.</creatorcontrib><creatorcontrib>Leinonen, M.</creatorcontrib><title>Type-specific enzyme immunoassay for detection of pneumococcal capsular polysaccharide antigens in nasopharyngeal specimens</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>We developed a new competitive EIA method for the demonstration of pneumococcal capsular polysaccharides from respiratory samples. The pediatric types 4, 6B, 9V, 14, 18C, 19F and 23F were selected for this study, because these capsular polysaccharides were included in the first heptavalent pneumococcal conjugate vaccines, which were used in the Finnish Otitis Media Vaccine Trial. Sensitivity of the EIA tests for purified polysaccharide antigens varied between 5 and 100 ng/ml, depending on the type. The assays performed well in 100 nasopharyngeal samples (NPS) samples processed through an enrichment culture, with an almost 100% sensitivity compared with routine culture. The method appeared type-specific, except that EIA for 6B capsule also detected 6A. The method is applicable for type-specific identification of pneumococcus in carriage studies.</description><subject>Antibodies, Bacterial - immunology</subject><subject>Antigens, Bacterial - analysis</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial Capsules - analysis</subject><subject>Bacterial Capsules - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Capsular polysaccharide antigens</subject><subject>Cohort Studies</subject><subject>Enzyme immunoassay</subject><subject>Finland</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques - methods</subject><subject>Infant</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Nasopharyngeal specimens</subject><subject>Nasopharynx - microbiology</subject><subject>Pneumococcal Infections - microbiology</subject><subject>Sensitivity and Specificity</subject><subject>Streptococcus pneumoniae</subject><subject>Streptococcus pneumoniae - immunology</subject><subject>Streptococcus pneumoniae - isolation & purification</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EotvCJ0BCvsAti_8lTg4cUAUUqRKXcracyaR4FdvBTpBSvjze7kq9gS-2nn8zY79HyBvO9pzx5sNh753HZS8Yk0XZM8GfkR1vtahaWXfPya5QutKMiwtymfOBMV5L1b4kF1xpVVa7I3_uthmrPCO40QHF8LB5pM77NUSbs93oGBMdcEFYXAw0jnQOuPoIEcBOFOyc18kmOsdpyxbgp01uQGrD4u4xZOoCDTbHuehbuMdS8jjMl7tX5MVop4yvz_sV-fHl8931TXX7_eu360-3FSjeLBVyAarrlKhrjaov5x4aBCEZNKK1_cAta5jsBfTIrO340MuBQa26tmuZHOUVeX_qO6f4a8W8GO8y4DTZgHHNpmWc61o0_wW57hpdvCugPIGQYs4JRzMn58sPDWfmGI45mMdwzDGco1jCKVVvz-3X3uPwVHNOowDvzoDNxdwx2QAuP3G10rrVXeE-njgsrv12mEwGhwFwcKnkZIbo_vmQv_TSsZw</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Lankinen, K.S.</creator><creator>Rintamäki, S.</creator><creator>Syrjänen, R.</creator><creator>Kilpi, T.</creator><creator>Ruutu, P.</creator><creator>Leinonen, M.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20040201</creationdate><title>Type-specific enzyme immunoassay for detection of pneumococcal capsular polysaccharide antigens in nasopharyngeal specimens</title><author>Lankinen, K.S. ; Rintamäki, S. ; Syrjänen, R. ; Kilpi, T. ; Ruutu, P. ; Leinonen, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-e12c49942557e4bc49bc6ec230c628abd1a0603b2cbe0aa91db3d0c54989803f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Antibodies, Bacterial - immunology</topic><topic>Antigens, Bacterial - analysis</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial Capsules - analysis</topic><topic>Bacterial Capsules - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Capsular polysaccharide antigens</topic><topic>Cohort Studies</topic><topic>Enzyme immunoassay</topic><topic>Finland</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques - methods</topic><topic>Infant</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Nasopharyngeal specimens</topic><topic>Nasopharynx - microbiology</topic><topic>Pneumococcal Infections - microbiology</topic><topic>Sensitivity and Specificity</topic><topic>Streptococcus pneumoniae</topic><topic>Streptococcus pneumoniae - immunology</topic><topic>Streptococcus pneumoniae - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lankinen, K.S.</creatorcontrib><creatorcontrib>Rintamäki, S.</creatorcontrib><creatorcontrib>Syrjänen, R.</creatorcontrib><creatorcontrib>Kilpi, T.</creatorcontrib><creatorcontrib>Ruutu, P.</creatorcontrib><creatorcontrib>Leinonen, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lankinen, K.S.</au><au>Rintamäki, S.</au><au>Syrjänen, R.</au><au>Kilpi, T.</au><au>Ruutu, P.</au><au>Leinonen, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Type-specific enzyme immunoassay for detection of pneumococcal capsular polysaccharide antigens in nasopharyngeal specimens</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2004-02-01</date><risdate>2004</risdate><volume>56</volume><issue>2</issue><spage>193</spage><epage>199</epage><pages>193-199</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>We developed a new competitive EIA method for the demonstration of pneumococcal capsular polysaccharides from respiratory samples. The pediatric types 4, 6B, 9V, 14, 18C, 19F and 23F were selected for this study, because these capsular polysaccharides were included in the first heptavalent pneumococcal conjugate vaccines, which were used in the Finnish Otitis Media Vaccine Trial. Sensitivity of the EIA tests for purified polysaccharide antigens varied between 5 and 100 ng/ml, depending on the type. The assays performed well in 100 nasopharyngeal samples (NPS) samples processed through an enrichment culture, with an almost 100% sensitivity compared with routine culture. The method appeared type-specific, except that EIA for 6B capsule also detected 6A. The method is applicable for type-specific identification of pneumococcus in carriage studies.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>14744448</pmid><doi>10.1016/j.mimet.2003.10.021</doi><tpages>7</tpages></addata></record> |
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| subjects | Antibodies, Bacterial - immunology Antigens, Bacterial - analysis Antigens, Bacterial - immunology Bacterial Capsules - analysis Bacterial Capsules - immunology Bacteriology Biological and medical sciences Capsular polysaccharide antigens Cohort Studies Enzyme immunoassay Finland Fundamental and applied biological sciences. Psychology Humans Immunoenzyme Techniques - methods Infant Microbiology Miscellaneous Nasopharyngeal specimens Nasopharynx - microbiology Pneumococcal Infections - microbiology Sensitivity and Specificity Streptococcus pneumoniae Streptococcus pneumoniae - immunology Streptococcus pneumoniae - isolation & purification |
| title | Type-specific enzyme immunoassay for detection of pneumococcal capsular polysaccharide antigens in nasopharyngeal specimens |
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