Neutral endopeptidase (EC 3.4.24.11) is highly expressed on osteoblastic cells and other marrow stromal cell types
A series of cultured mouse marrow stromal cell lines of different phenotype characteristics were examined for their neutral endopeptidase (NEP) activity using the highly selective chromophoric substrate of the enzyme, 3-carboxypropanoyl-alanyl-alanyl-leucine 4-nitroanilide. All the cell lines tested...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1990-10, Vol.172 (2), p.620-626 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Indig, Fred E. Benayahu, Daphna Fried, Adina Wientroub, Shlomo Blumberg, Shmaryahu |
| description | A series of cultured mouse marrow stromal cell lines of different phenotype characteristics were examined for their neutral endopeptidase (NEP) activity using the highly selective chromophoric substrate of the enzyme, 3-carboxypropanoyl-alanyl-alanyl-leucine 4-nitroanilide. All the cell lines tested contained appreciable amounts of NEP activity, but the cells expressing an osteoblastic phenotype, MBA-15, showed the highest levels. Other non-stromal osteoblastic cell lines, MC-3T3-E1, ROS 17/2.8 and cells derived from mouse long bone explants, were also rich in NEP. A four-fold stimulation of NEP activity was observed when certain MBA-15 clones were cultured in the presence of 1α,25-dihydroxyvitamin D
3. Since NEP is an effective cell-surface endopeptidase, it may play a role in the dynamics of bone formation, via interaction with biologically active polypeptides. |
| doi_str_mv | 10.1016/0006-291X(90)90719-4 |
| format | Article |
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3. Since NEP is an effective cell-surface endopeptidase, it may play a role in the dynamics of bone formation, via interaction with biologically active polypeptides.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>bone</subject><subject>bone growth</subject><subject>bone marrow</subject><subject>Bone Marrow - enzymology</subject><subject>Bone Marrow Cells</subject><subject>Calcitriol - pharmacology</subject><subject>Cell Line</subject><subject>Cell Membrane - enzymology</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolases</subject><subject>Kinetics</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Neprilysin - metabolism</subject><subject>osteoblasts</subject><subject>Osteoblasts - enzymology</subject><subject>Rats</subject><subject>Substrate Specificity</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1vEzEQtRBVCYV_AJIvoPawYfyR3fUFCUUFKlVwAYmb5bUnxGizXjwOkH_PLonaWzmM5vDePL15j7EXApYCRP0GAOpKGvHt0sCVgUaYSj9iCwEGKilAP2aLO8oT9pToB4AQujbn7FxKLcyqWbD8Cfclu57jENKIY4nBEfLL6zVXS72UeinEFY_Et_H7tj9w_DNmJMLA08ATFUxd76hEzz32PXE3TEjZYuY7l3P6zanktJv0Z5iXw4j0jJ1tXE_4_LQv2Nf311_WH6vbzx9u1u9uK69FWypUjaxDrTsdgpdeInZGtgJD2BhQjQ6tEFKhV07paQyEVdOh8NgaAIVGXbDXR90xp597pGJ3kWYbbsC0J9tOadS1av9LFKtWrVopJ6I-En1ORBk3dsxx-vNgBdi5EzsHbufArQH7rxOrp7OXJ_19t8Nwd3QqYcJfnXBH3vWb7AYf6V7b1CBrOft8e-ThlNqviNmSjzh4DDGjLzak-LCRv_Jbp38</recordid><startdate>19901030</startdate><enddate>19901030</enddate><creator>Indig, Fred E.</creator><creator>Benayahu, Daphna</creator><creator>Fried, Adina</creator><creator>Wientroub, Shlomo</creator><creator>Blumberg, Shmaryahu</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19901030</creationdate><title>Neutral endopeptidase (EC 3.4.24.11) is highly expressed on osteoblastic cells and other marrow stromal cell types</title><author>Indig, Fred E. ; Benayahu, Daphna ; Fried, Adina ; Wientroub, Shlomo ; Blumberg, Shmaryahu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-e3726d64b4ddc2c2eeb9281eddf90374d81123ec3a343a390d57be1ce89003e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>bone</topic><topic>bone growth</topic><topic>bone marrow</topic><topic>Bone Marrow - enzymology</topic><topic>Bone Marrow Cells</topic><topic>Calcitriol - pharmacology</topic><topic>Cell Line</topic><topic>Cell Membrane - enzymology</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolases</topic><topic>Kinetics</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Neprilysin - metabolism</topic><topic>osteoblasts</topic><topic>Osteoblasts - enzymology</topic><topic>Rats</topic><topic>Substrate Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Indig, Fred E.</creatorcontrib><creatorcontrib>Benayahu, Daphna</creatorcontrib><creatorcontrib>Fried, Adina</creatorcontrib><creatorcontrib>Wientroub, Shlomo</creatorcontrib><creatorcontrib>Blumberg, Shmaryahu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Indig, Fred E.</au><au>Benayahu, Daphna</au><au>Fried, Adina</au><au>Wientroub, Shlomo</au><au>Blumberg, Shmaryahu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutral endopeptidase (EC 3.4.24.11) is highly expressed on osteoblastic cells and other marrow stromal cell types</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1990-10-30</date><risdate>1990</risdate><volume>172</volume><issue>2</issue><spage>620</spage><epage>626</epage><pages>620-626</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>A series of cultured mouse marrow stromal cell lines of different phenotype characteristics were examined for their neutral endopeptidase (NEP) activity using the highly selective chromophoric substrate of the enzyme, 3-carboxypropanoyl-alanyl-alanyl-leucine 4-nitroanilide. All the cell lines tested contained appreciable amounts of NEP activity, but the cells expressing an osteoblastic phenotype, MBA-15, showed the highest levels. Other non-stromal osteoblastic cell lines, MC-3T3-E1, ROS 17/2.8 and cells derived from mouse long bone explants, were also rich in NEP. A four-fold stimulation of NEP activity was observed when certain MBA-15 clones were cultured in the presence of 1α,25-dihydroxyvitamin D
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| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Biological and medical sciences bone bone growth bone marrow Bone Marrow - enzymology Bone Marrow Cells Calcitriol - pharmacology Cell Line Cell Membrane - enzymology Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Kinetics Mice Molecular Sequence Data Neprilysin - metabolism osteoblasts Osteoblasts - enzymology Rats Substrate Specificity |
| title | Neutral endopeptidase (EC 3.4.24.11) is highly expressed on osteoblastic cells and other marrow stromal cell types |
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