Galectin-1, a cell adhesion modulator, induces apoptosis of rat Leydig cells in vitro

Galectin-1 (Gal-1), a beta galactoside-binding lectin, is involved in multiple biological functions, such as cell adhesion, apoptosis, and metastasis. On the basis of its ability to interact with extracellular matrix (ECM) glycoproteins, we investigated the Gal-1 effect on Leydig cells, which expres...

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Veröffentlicht in:	Glycobiology (Oxford) 2004-02, Vol.14 (2), p.127-137
Hauptverfasser:	Martinez, Vanesa G., Pellizzari, Eliana H., Díaz, Emilce S., Cigorraga, Selva B., Lustig, Livia, Denduchis, Berta, Wolfenstein-Todel, Carlota, Iglesias, M. Mercedes
Format:	Artikel
Sprache:	eng
Schlagworte:	3- 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium Animals apoptosis Apoptosis - drug effects bovine serum albumin BSA carbohydrate-recognition domain Caspase 3 Caspases - metabolism Cell Adhesion Cell Survival Cells, Cultured Colorimetry CRD Culture Media dithiothreitol DMEM DTT Dulbecco's modified Eagle medium ECM extracellular matrix FITC Flow Cytometry fluorescein isothiocyanate Fluorescent Antibody Technique Galectin 1 - pharmacology galectin-1 Gen genistein hCG human chorionic gonadotrophin In Vitro Techniques inner salt) and phenazine methosulfate laminin-1 Leydig cells Leydig Cells - drug effects Leydig Cells - ultrastructure Male methylprednisolone MTS paraformaldehyde PBS phosphate buffered saline Rats Rats, Sprague-Dawley testis Testis - drug effects Testis - metabolism Testosterone - metabolism
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