Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands
1 Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia 2 Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia 3 Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany Correspon...
Gespeichert in:
| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2004-01, Vol.54 (1), p.151-156 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 156 |
|---|---|
| container_issue | 1 |
| container_start_page | 151 |
| container_title | International journal of systematic and evolutionary microbiology |
| container_volume | 54 |
| creator | Dedysh, Svetlana N Berestovskaya, Yulia Y Vasylieva, Lina V Belova, Svetlana E Khmelenina, Valentina N Suzina, Natalia E Trotsenko, Yuri A Liesack, Werner Zavarzin, George A |
| description | 1 Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia
2 Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia
3 Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany
Correspondence Svetlana N. Dedysh s.dedysh{at}mtu-net.ru
A novel species, Methylocella tundrae , is proposed for three methanotrophic strains (T4 T , TCh1 and TY1) isolated from acidic Sphagnum tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4 T , TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4·2 and 7·5 (optimum 5·56·0) and between 5 and 30 °C (optimum 15 °C). The major phospholipid fatty acid is 18 : 1 7 c . The DNA G+C content of strain T4 T is 63·3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of Methylocella , Methylocella palustris (97 % similarity) and Methylocella silvestris (97·5 % similarity). DNADNA hybridization values of strain T4 T with Methylocella palustris K T and Methylocella silvestris BL2 T were respectively 27 and 36 %. Thus, the tundra strains represent a novel species, for which the name Methylocella tundrae sp. nov. is proposed. Strain T4 T (=DSM 15673 T =NCIMB 13949 T ) is the type strain.
Abbreviations: ICM, intracytoplasmic membrane; PLFA, phospholipid fatty acid; sMMO, soluble methane monooxygenase
Published online ahead of print on 25 July 2003 as DOI 10.1099/ijs.0.02805-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and partial sequences of the mmoX and mxaF genes of Methylocella tundrae strain T4 T are respectively AJ555244 , AJ555245 and AJ555246 . |
| doi_str_mv | 10.1099/ijs.0.02805-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80113675</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19267497</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-54ed470eeaac679aab417cc7ff9274c30042262c573ccb851b51a29736030b4d3</originalsourceid><addsrcrecordid>eNqFkE1LxDAQhoMorq4evUpOerF18tVsj7L4BYoXvQkhTVM3S9vUpFX893btokdP7zA888C8CJ0QSAnk-aVbxxRSoAsQCeygA8KzLBHA-O7vTLMZOoxxDTAuAPbRjHDJKZfsAL0-2n71VXtj61rjfmjLoC2OXYpb_5FeYL1JW-NmxHTr--C7lTO40Ka3wQ0NroJvsDauHLfTOe6s7mvdlvEI7VW6jvZ4m3P0cnP9vLxLHp5u75dXD4nhQvaJ4LbkEqzV2mQy17rgRBojqyqnkhsGwCnNqBGSGVMsBCkE0TSXLAMGBS_ZHJ1N3i7498HGXjUu_nzUWj9EtQBCWCbFvyDJaSb5aJ6jZAJN8DEGW6kuuEaHL0VAbXpXY-8K1E_vCkb-dCseisaWf_S26BE4n4CVe1t9umDVm20bN-oL5zcywRVRRBD2DZjhjGg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19267497</pqid></control><display><type>article</type><title>Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands</title><source>MEDLINE</source><source>Microbiology Society</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Dedysh, Svetlana N ; Berestovskaya, Yulia Y ; Vasylieva, Lina V ; Belova, Svetlana E ; Khmelenina, Valentina N ; Suzina, Natalia E ; Trotsenko, Yuri A ; Liesack, Werner ; Zavarzin, George A</creator><creatorcontrib>Dedysh, Svetlana N ; Berestovskaya, Yulia Y ; Vasylieva, Lina V ; Belova, Svetlana E ; Khmelenina, Valentina N ; Suzina, Natalia E ; Trotsenko, Yuri A ; Liesack, Werner ; Zavarzin, George A</creatorcontrib><description>1 Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia
2 Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia
3 Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany
Correspondence Svetlana N. Dedysh s.dedysh{at}mtu-net.ru
A novel species, Methylocella tundrae , is proposed for three methanotrophic strains (T4 T , TCh1 and TY1) isolated from acidic Sphagnum tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4 T , TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4·2 and 7·5 (optimum 5·56·0) and between 5 and 30 °C (optimum 15 °C). The major phospholipid fatty acid is 18 : 1 7 c . The DNA G+C content of strain T4 T is 63·3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of Methylocella , Methylocella palustris (97 % similarity) and Methylocella silvestris (97·5 % similarity). DNADNA hybridization values of strain T4 T with Methylocella palustris K T and Methylocella silvestris BL2 T were respectively 27 and 36 %. Thus, the tundra strains represent a novel species, for which the name Methylocella tundrae sp. nov. is proposed. Strain T4 T (=DSM 15673 T =NCIMB 13949 T ) is the type strain.
Abbreviations: ICM, intracytoplasmic membrane; PLFA, phospholipid fatty acid; sMMO, soluble methane monooxygenase
Published online ahead of print on 25 July 2003 as DOI 10.1099/ijs.0.02805-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and partial sequences of the mmoX and mxaF genes of Methylocella tundrae strain T4 T are respectively AJ555244 , AJ555245 and AJ555246 .</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.02805-0</identifier><identifier>PMID: 14742473</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Carbon - metabolism ; Cold Climate ; Enzymes - metabolism ; Fatty Acids - analysis ; Hydrogen-Ion Concentration ; Methylocella tundrae ; Molecular Sequence Data ; Phylogeny ; Rhizobiaceae - classification ; Rhizobiaceae - cytology ; Rhizobiaceae - genetics ; Rhizobiaceae - isolation & purification ; Russia ; Serine - metabolism ; Soil Microbiology ; Sphagnum</subject><ispartof>International journal of systematic and evolutionary microbiology, 2004-01, Vol.54 (1), p.151-156</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-54ed470eeaac679aab417cc7ff9274c30042262c573ccb851b51a29736030b4d3</citedby><cites>FETCH-LOGICAL-c457t-54ed470eeaac679aab417cc7ff9274c30042262c573ccb851b51a29736030b4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14742473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dedysh, Svetlana N</creatorcontrib><creatorcontrib>Berestovskaya, Yulia Y</creatorcontrib><creatorcontrib>Vasylieva, Lina V</creatorcontrib><creatorcontrib>Belova, Svetlana E</creatorcontrib><creatorcontrib>Khmelenina, Valentina N</creatorcontrib><creatorcontrib>Suzina, Natalia E</creatorcontrib><creatorcontrib>Trotsenko, Yuri A</creatorcontrib><creatorcontrib>Liesack, Werner</creatorcontrib><creatorcontrib>Zavarzin, George A</creatorcontrib><title>Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>1 Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia
2 Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia
3 Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany
Correspondence Svetlana N. Dedysh s.dedysh{at}mtu-net.ru
A novel species, Methylocella tundrae , is proposed for three methanotrophic strains (T4 T , TCh1 and TY1) isolated from acidic Sphagnum tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4 T , TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4·2 and 7·5 (optimum 5·56·0) and between 5 and 30 °C (optimum 15 °C). The major phospholipid fatty acid is 18 : 1 7 c . The DNA G+C content of strain T4 T is 63·3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of Methylocella , Methylocella palustris (97 % similarity) and Methylocella silvestris (97·5 % similarity). DNADNA hybridization values of strain T4 T with Methylocella palustris K T and Methylocella silvestris BL2 T were respectively 27 and 36 %. Thus, the tundra strains represent a novel species, for which the name Methylocella tundrae sp. nov. is proposed. Strain T4 T (=DSM 15673 T =NCIMB 13949 T ) is the type strain.
Abbreviations: ICM, intracytoplasmic membrane; PLFA, phospholipid fatty acid; sMMO, soluble methane monooxygenase
Published online ahead of print on 25 July 2003 as DOI 10.1099/ijs.0.02805-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and partial sequences of the mmoX and mxaF genes of Methylocella tundrae strain T4 T are respectively AJ555244 , AJ555245 and AJ555246 .</description><subject>Carbon - metabolism</subject><subject>Cold Climate</subject><subject>Enzymes - metabolism</subject><subject>Fatty Acids - analysis</subject><subject>Hydrogen-Ion Concentration</subject><subject>Methylocella tundrae</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Rhizobiaceae - classification</subject><subject>Rhizobiaceae - cytology</subject><subject>Rhizobiaceae - genetics</subject><subject>Rhizobiaceae - isolation & purification</subject><subject>Russia</subject><subject>Serine - metabolism</subject><subject>Soil Microbiology</subject><subject>Sphagnum</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMorq4evUpOerF18tVsj7L4BYoXvQkhTVM3S9vUpFX893btokdP7zA888C8CJ0QSAnk-aVbxxRSoAsQCeygA8KzLBHA-O7vTLMZOoxxDTAuAPbRjHDJKZfsAL0-2n71VXtj61rjfmjLoC2OXYpb_5FeYL1JW-NmxHTr--C7lTO40Ka3wQ0NroJvsDauHLfTOe6s7mvdlvEI7VW6jvZ4m3P0cnP9vLxLHp5u75dXD4nhQvaJ4LbkEqzV2mQy17rgRBojqyqnkhsGwCnNqBGSGVMsBCkE0TSXLAMGBS_ZHJ1N3i7498HGXjUu_nzUWj9EtQBCWCbFvyDJaSb5aJ6jZAJN8DEGW6kuuEaHL0VAbXpXY-8K1E_vCkb-dCseisaWf_S26BE4n4CVe1t9umDVm20bN-oL5zcywRVRRBD2DZjhjGg</recordid><startdate>20040101</startdate><enddate>20040101</enddate><creator>Dedysh, Svetlana N</creator><creator>Berestovskaya, Yulia Y</creator><creator>Vasylieva, Lina V</creator><creator>Belova, Svetlana E</creator><creator>Khmelenina, Valentina N</creator><creator>Suzina, Natalia E</creator><creator>Trotsenko, Yuri A</creator><creator>Liesack, Werner</creator><creator>Zavarzin, George A</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20040101</creationdate><title>Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands</title><author>Dedysh, Svetlana N ; Berestovskaya, Yulia Y ; Vasylieva, Lina V ; Belova, Svetlana E ; Khmelenina, Valentina N ; Suzina, Natalia E ; Trotsenko, Yuri A ; Liesack, Werner ; Zavarzin, George A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-54ed470eeaac679aab417cc7ff9274c30042262c573ccb851b51a29736030b4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Carbon - metabolism</topic><topic>Cold Climate</topic><topic>Enzymes - metabolism</topic><topic>Fatty Acids - analysis</topic><topic>Hydrogen-Ion Concentration</topic><topic>Methylocella tundrae</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Rhizobiaceae - classification</topic><topic>Rhizobiaceae - cytology</topic><topic>Rhizobiaceae - genetics</topic><topic>Rhizobiaceae - isolation & purification</topic><topic>Russia</topic><topic>Serine - metabolism</topic><topic>Soil Microbiology</topic><topic>Sphagnum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dedysh, Svetlana N</creatorcontrib><creatorcontrib>Berestovskaya, Yulia Y</creatorcontrib><creatorcontrib>Vasylieva, Lina V</creatorcontrib><creatorcontrib>Belova, Svetlana E</creatorcontrib><creatorcontrib>Khmelenina, Valentina N</creatorcontrib><creatorcontrib>Suzina, Natalia E</creatorcontrib><creatorcontrib>Trotsenko, Yuri A</creatorcontrib><creatorcontrib>Liesack, Werner</creatorcontrib><creatorcontrib>Zavarzin, George A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dedysh, Svetlana N</au><au>Berestovskaya, Yulia Y</au><au>Vasylieva, Lina V</au><au>Belova, Svetlana E</au><au>Khmelenina, Valentina N</au><au>Suzina, Natalia E</au><au>Trotsenko, Yuri A</au><au>Liesack, Werner</au><au>Zavarzin, George A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2004-01-01</date><risdate>2004</risdate><volume>54</volume><issue>1</issue><spage>151</spage><epage>156</epage><pages>151-156</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>1 Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia
2 Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia
3 Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany
Correspondence Svetlana N. Dedysh s.dedysh{at}mtu-net.ru
A novel species, Methylocella tundrae , is proposed for three methanotrophic strains (T4 T , TCh1 and TY1) isolated from acidic Sphagnum tundra peatlands. These strains are aerobic, Gram-negative, non-motile, dinitrogen-fixing rods that possess a soluble methane monooxygenase and utilize the serine pathway for carbon assimilation. Strains T4 T , TCh1 and TY1 are moderately acidophilic organisms capable of growth between pH 4·2 and 7·5 (optimum 5·56·0) and between 5 and 30 °C (optimum 15 °C). The major phospholipid fatty acid is 18 : 1 7 c . The DNA G+C content of strain T4 T is 63·3 mol%. The three strains possess almost identical 16S rRNA gene sequences and are most closely related to two previously identified species of Methylocella , Methylocella palustris (97 % similarity) and Methylocella silvestris (97·5 % similarity). DNADNA hybridization values of strain T4 T with Methylocella palustris K T and Methylocella silvestris BL2 T were respectively 27 and 36 %. Thus, the tundra strains represent a novel species, for which the name Methylocella tundrae sp. nov. is proposed. Strain T4 T (=DSM 15673 T =NCIMB 13949 T ) is the type strain.
Abbreviations: ICM, intracytoplasmic membrane; PLFA, phospholipid fatty acid; sMMO, soluble methane monooxygenase
Published online ahead of print on 25 July 2003 as DOI 10.1099/ijs.0.02805-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and partial sequences of the mmoX and mxaF genes of Methylocella tundrae strain T4 T are respectively AJ555244 , AJ555245 and AJ555246 .</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>14742473</pmid><doi>10.1099/ijs.0.02805-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2004-01, Vol.54 (1), p.151-156 |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80113675 |
| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Carbon - metabolism Cold Climate Enzymes - metabolism Fatty Acids - analysis Hydrogen-Ion Concentration Methylocella tundrae Molecular Sequence Data Phylogeny Rhizobiaceae - classification Rhizobiaceae - cytology Rhizobiaceae - genetics Rhizobiaceae - isolation & purification Russia Serine - metabolism Soil Microbiology Sphagnum |
| title | Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T04%3A40%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Methylocella%20tundrae%20sp.%20nov.,%20a%20novel%20methanotrophic%20bacterium%20from%20acidic%20tundra%20peatlands&rft.jtitle=International%20journal%20of%20systematic%20and%20evolutionary%20microbiology&rft.au=Dedysh,%20Svetlana%20N&rft.date=2004-01-01&rft.volume=54&rft.issue=1&rft.spage=151&rft.epage=156&rft.pages=151-156&rft.issn=1466-5026&rft.eissn=1466-5034&rft_id=info:doi/10.1099/ijs.0.02805-0&rft_dat=%3Cproquest_cross%3E19267497%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19267497&rft_id=info:pmid/14742473&rfr_iscdi=true |