Profiling the allosteric response of an engineered β-galactosidase to its effector, anti-HIV antibody

Escherichia coli β-galactosidase responds enzymatically to antiviral antibodies when a viral antigenic peptide, acting as receptor, is conveniently displayed in the vicinity of the active site. The allosteric response of a β-galactosidase molecular sensor containing a B-cell epitope from HIV has bee...

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Veröffentlicht in:	Biochemical and biophysical research communications 2004-02, Vol.314 (3), p.854-860
Hauptverfasser:	Ferraz, Rosa M, Arı́s, Anna, Villaverde, Antonio
Format:	Artikel
Sprache:	eng
Schlagworte:	Allosteric enzyme Allosteric Regulation Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - metabolism Antibody Beta-galactosidase beta-Galactosidase - genetics beta-Galactosidase - immunology beta-Galactosidase - metabolism Biosensing Techniques - methods Biosensor Enzyme Activation - immunology Enzyme-Linked Immunosorbent Assay - methods Escherichia coli - metabolism Fluorescent Antibody Technique HIV HIV Antibodies - immunology HIV Antibodies - metabolism Human immunodeficiency virus Humans Kinetics Nitrophenylgalactosides - chemistry Nitrophenylgalactosides - metabolism Protein Engineering Recombinant Proteins - immunology Recombinant Proteins - metabolism Signal transduction
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