Evidence that cleavage of the precursor enzyme by autocatalysis caused secretion of multiple amylases by Aspergillus niger
The observation that a mutant strain of Aspergillus niger isolated for protease overproduction accumulated Taka-amylase supported an earlier report that processing of the precursor amylase by protease resulted in the secretion of multiple amylases. Studies using a mutant strain revealed that such pr...
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| Veröffentlicht in: | FEBS letters 2004-01, Vol.557 (1), p.239-242 |
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| creator | Ravi-Kumar, K Venkatesh, K.S Umesh-Kumar, S |
| description | The observation that a mutant strain of
Aspergillus niger isolated for protease overproduction accumulated Taka-amylase supported an earlier report that processing of the precursor amylase by protease resulted in the secretion of multiple amylases. Studies using a mutant strain revealed that such processing was not due to aspergillopepsin but to autocatalysis by an inherent protease activity of the precursor and glucoamylase. Alignment of protease sequences with glucoamylase showed regions of consensus with serine carboxypeptidase of
A. niger. Thus point mutations in this region due to ultraviolet radiation apparently caused the mutant to evolve with enhanced protease activity that degraded the precursor and accumulated Taka-amylase. |
| doi_str_mv | 10.1016/S0014-5793(03)01510-2 |
| format | Article |
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Aspergillus niger isolated for protease overproduction accumulated Taka-amylase supported an earlier report that processing of the precursor amylase by protease resulted in the secretion of multiple amylases. Studies using a mutant strain revealed that such processing was not due to aspergillopepsin but to autocatalysis by an inherent protease activity of the precursor and glucoamylase. Alignment of protease sequences with glucoamylase showed regions of consensus with serine carboxypeptidase of
A. niger. Thus point mutations in this region due to ultraviolet radiation apparently caused the mutant to evolve with enhanced protease activity that degraded the precursor and accumulated Taka-amylase.</description><subject>Amylases - isolation & purification</subject><subject>Amylases - metabolism</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - enzymology</subject><subject>Aspergillus niger - physiology</subject><subject>Carboxypeptidase</subject><subject>Catalysis</subject><subject>Enzyme Precursors - metabolism</subject><subject>Glucoamylase</subject><subject>Isoenzymes - isolation & purification</subject><subject>Isoenzymes - metabolism</subject><subject>Precursor</subject><subject>Spores, Fungal - physiology</subject><subject>Taka-amylase</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-PFCEQxYnRuOPqR9BwMnpohQYG-mTWzaxrsokH9UxoKEYM_UfoHtP76W1mJnpcExJC1e-9IvUQeknJO0ro9v1XQiivhGzYG8LeEiooqepHaEOVZBXjW_UYbf4iF-hZzj_J-la0eYouKJecMsk36H53CA56C3j6YSZsI5iD2QMe_FoAPCawc8pDwtDfLx3gdsFmngZrJhOXHDK2Zs7gcAabYApDX5TdHKcwRsCmW6LJkIvsKo-Q9iHGOeM-7CE9R0-8iRlenO9L9P1m9-36trr78unz9dVdZbmQdcUapmTdNq1nFHjdUOMbJT046b2AlnuxbRg3UhBQUlrRGratwShn14Jzgl2i1yffMQ2_ZsiT7kK2EKPpYZizVoTSmon6QZBKIqTiBRQn0KYh5wRejyl0Ji2aEl3S0cd0dFm9Jusp6eiie3UeMLcduH-qcxwrcHsCfocIy_-56pvdx_rYKQ3CjuUy68PJCtbVHgIknW0oQbuwZjppN4QHfvsH4_C1CQ</recordid><startdate>20040116</startdate><enddate>20040116</enddate><creator>Ravi-Kumar, K</creator><creator>Venkatesh, K.S</creator><creator>Umesh-Kumar, S</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20040116</creationdate><title>Evidence that cleavage of the precursor enzyme by autocatalysis caused secretion of multiple amylases by Aspergillus niger</title><author>Ravi-Kumar, K ; Venkatesh, K.S ; Umesh-Kumar, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4572-393872b9bf31e4291af987fed7ff5eb4f56934a750e877c5ba362ea8dc0e8dd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amylases - isolation & purification</topic><topic>Amylases - metabolism</topic><topic>Aspergillus niger</topic><topic>Aspergillus niger - enzymology</topic><topic>Aspergillus niger - physiology</topic><topic>Carboxypeptidase</topic><topic>Catalysis</topic><topic>Enzyme Precursors - metabolism</topic><topic>Glucoamylase</topic><topic>Isoenzymes - isolation & purification</topic><topic>Isoenzymes - metabolism</topic><topic>Precursor</topic><topic>Spores, Fungal - physiology</topic><topic>Taka-amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ravi-Kumar, K</creatorcontrib><creatorcontrib>Venkatesh, K.S</creatorcontrib><creatorcontrib>Umesh-Kumar, S</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ravi-Kumar, K</au><au>Venkatesh, K.S</au><au>Umesh-Kumar, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence that cleavage of the precursor enzyme by autocatalysis caused secretion of multiple amylases by Aspergillus niger</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2004-01-16</date><risdate>2004</risdate><volume>557</volume><issue>1</issue><spage>239</spage><epage>242</epage><pages>239-242</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The observation that a mutant strain of
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| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2004-01, Vol.557 (1), p.239-242 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80112352 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Amylases - isolation & purification Amylases - metabolism Aspergillus niger Aspergillus niger - enzymology Aspergillus niger - physiology Carboxypeptidase Catalysis Enzyme Precursors - metabolism Glucoamylase Isoenzymes - isolation & purification Isoenzymes - metabolism Precursor Spores, Fungal - physiology Taka-amylase |
| title | Evidence that cleavage of the precursor enzyme by autocatalysis caused secretion of multiple amylases by Aspergillus niger |
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