Genomic organization and regulation of the LeIMP-1 and LeIMP-2 genes encoding myo-inositol monophosphatase in tomato
Myo-inositol (inositol) monophosphatase (IMP), an enzyme which catalyzes the synthesis of free inositol from various inositol monophosphates, is encoded by a small multigene family in many organisms. The tomato IMP gene family encodes three IMP isoforms with identical in vitro biochemical properties...
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| Veröffentlicht in: | Gene 2004-02, Vol.326, p.35-41 |
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| creator | Styer, Jean C. Keddie, James Spence, Jeremiah Gillaspy, Glenda E. |
| description | Myo-inositol (inositol) monophosphatase (IMP), an enzyme which catalyzes the synthesis of free inositol from various inositol monophosphates, is encoded by a small multigene family in many organisms. The tomato IMP gene family encodes three IMP isoforms with identical in vitro biochemical properties. To determine the role of each tomato
LeIMP gene in plant growth, we isolated the genomic DNA copies of the
LeIMP-1 and
LeIMP-2 genes. The
LeIMP-1 gene spans approximately 5.8 kb and consists of 12 exons, whereas the
LeIMP-2 gene consists of an uninterrupted, single open reading frame (ORF). We have previously shown that steady-state levels of
LeIMP-2 mRNA were very low in comparison to
LeIMP-1 and
LeIMP-3 mRNA levels. To determine whether
LeIMP-2 gene expression was spatially restricted to a discreet domain within the plant we constructed transgenic plants containing an
LeIMP-2 promoter::uidA gene fusion. Analysis of transgenic seedlings revealed that the
LeIMP-2 promoter directed gene expression within epidermal and cortex cells of specific stem/leaf junctions in an abaxial-specific pattern and in the shoot apical meristem. Further, inositol, the product of IMP catalysis, and Li
+, an inhibitor of IMP catalysis, decreased expression of the
LeIMP-2 promoter as measured by a decrease in β-glucuronidase activity after treatment. |
| doi_str_mv | 10.1016/j.gene.2003.09.048 |
| format | Article |
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LeIMP gene in plant growth, we isolated the genomic DNA copies of the
LeIMP-1 and
LeIMP-2 genes. The
LeIMP-1 gene spans approximately 5.8 kb and consists of 12 exons, whereas the
LeIMP-2 gene consists of an uninterrupted, single open reading frame (ORF). We have previously shown that steady-state levels of
LeIMP-2 mRNA were very low in comparison to
LeIMP-1 and
LeIMP-3 mRNA levels. To determine whether
LeIMP-2 gene expression was spatially restricted to a discreet domain within the plant we constructed transgenic plants containing an
LeIMP-2 promoter::uidA gene fusion. Analysis of transgenic seedlings revealed that the
LeIMP-2 promoter directed gene expression within epidermal and cortex cells of specific stem/leaf junctions in an abaxial-specific pattern and in the shoot apical meristem. Further, inositol, the product of IMP catalysis, and Li
+, an inhibitor of IMP catalysis, decreased expression of the
LeIMP-2 promoter as measured by a decrease in β-glucuronidase activity after treatment.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2003.09.048</identifier><identifier>PMID: 14729261</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; DNA, Plant - chemistry ; DNA, Plant - genetics ; Exons ; Genes, Plant - genetics ; Glucuronidase - genetics ; Glucuronidase - metabolism ; Inositol - pharmacology ; Intronless ; Introns ; Isoenzymes - genetics ; Lithium - pharmacology ; Lycopersicon esculentum ; Lycopersicon esculentum - enzymology ; Lycopersicon esculentum - genetics ; Molecular Sequence Data ; Phosphoric Monoester Hydrolases - genetics ; Phylogeny ; Plants, Genetically Modified ; Promoter Regions, Genetic - genetics ; Recombinant Fusion Proteins - drug effects ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Sequence Analysis, DNA ; Transgenic plant ; β-Glucuronidase</subject><ispartof>Gene, 2004-02, Vol.326, p.35-41</ispartof><rights>2003 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-d3e1e0965c43b277c9aee4e659746e26496ca670a3dd72e394de699d31ce4cce3</citedby><cites>FETCH-LOGICAL-c383t-d3e1e0965c43b277c9aee4e659746e26496ca670a3dd72e394de699d31ce4cce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gene.2003.09.048$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14729261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Styer, Jean C.</creatorcontrib><creatorcontrib>Keddie, James</creatorcontrib><creatorcontrib>Spence, Jeremiah</creatorcontrib><creatorcontrib>Gillaspy, Glenda E.</creatorcontrib><title>Genomic organization and regulation of the LeIMP-1 and LeIMP-2 genes encoding myo-inositol monophosphatase in tomato</title><title>Gene</title><addtitle>Gene</addtitle><description>Myo-inositol (inositol) monophosphatase (IMP), an enzyme which catalyzes the synthesis of free inositol from various inositol monophosphates, is encoded by a small multigene family in many organisms. The tomato IMP gene family encodes three IMP isoforms with identical in vitro biochemical properties. To determine the role of each tomato
LeIMP gene in plant growth, we isolated the genomic DNA copies of the
LeIMP-1 and
LeIMP-2 genes. The
LeIMP-1 gene spans approximately 5.8 kb and consists of 12 exons, whereas the
LeIMP-2 gene consists of an uninterrupted, single open reading frame (ORF). We have previously shown that steady-state levels of
LeIMP-2 mRNA were very low in comparison to
LeIMP-1 and
LeIMP-3 mRNA levels. To determine whether
LeIMP-2 gene expression was spatially restricted to a discreet domain within the plant we constructed transgenic plants containing an
LeIMP-2 promoter::uidA gene fusion. Analysis of transgenic seedlings revealed that the
LeIMP-2 promoter directed gene expression within epidermal and cortex cells of specific stem/leaf junctions in an abaxial-specific pattern and in the shoot apical meristem. Further, inositol, the product of IMP catalysis, and Li
+, an inhibitor of IMP catalysis, decreased expression of the
LeIMP-2 promoter as measured by a decrease in β-glucuronidase activity after treatment.</description><subject>Animals</subject><subject>DNA, Plant - chemistry</subject><subject>DNA, Plant - genetics</subject><subject>Exons</subject><subject>Genes, Plant - genetics</subject><subject>Glucuronidase - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>Inositol - pharmacology</subject><subject>Intronless</subject><subject>Introns</subject><subject>Isoenzymes - genetics</subject><subject>Lithium - pharmacology</subject><subject>Lycopersicon esculentum</subject><subject>Lycopersicon esculentum - enzymology</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Molecular Sequence Data</subject><subject>Phosphoric Monoester Hydrolases - genetics</subject><subject>Phylogeny</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant Fusion Proteins - drug effects</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Transgenic plant</subject><subject>β-Glucuronidase</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vEzEQxS1ERUPhC3BAPnHbxf_WXktcUFVKpSB6aM-Wa08SR7t2sB2k8ulx2EjcwBd7xr95Gr2H0DtKekqo_LjvtxChZ4TwnuieiPEFWtFR6a51xpdoRbgaO0qpvkSvS9mTdoaBvUKXVCimmaQrVG8hpjk4nPLWxvDL1pAittHjDNvjtJRpg-sO8Bruvt139M_v8mb4tEHBEF3yIW7x_Jy6EFMJNU14TjEddqkcdrbaAjhEXNNsa3qDLjZ2KvD2fF-hxy83D9dfu_X327vrz-vO8ZHXznOgQLQcnOBPTCmnLYAAOWglJDAptHRWKmK594oB18KD1Npz6kA4B_wKfVh0Dzn9OEKpZg7FwTTZCOlYzEgoUc2R_4JUaT4oJRrIFtDlVEqGjTnkMNv8bCgxp1DM3pwsMadQDNGmhdKG3p_Vj08z-L8j5xQa8GkBoJnxM0A2xYXmKfiQwVXjU_iX_m-ZlZ5E</recordid><startdate>20040204</startdate><enddate>20040204</enddate><creator>Styer, Jean C.</creator><creator>Keddie, James</creator><creator>Spence, Jeremiah</creator><creator>Gillaspy, Glenda E.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040204</creationdate><title>Genomic organization and regulation of the LeIMP-1 and LeIMP-2 genes encoding myo-inositol monophosphatase in tomato</title><author>Styer, Jean C. ; Keddie, James ; Spence, Jeremiah ; Gillaspy, Glenda E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-d3e1e0965c43b277c9aee4e659746e26496ca670a3dd72e394de699d31ce4cce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>DNA, Plant - chemistry</topic><topic>DNA, Plant - genetics</topic><topic>Exons</topic><topic>Genes, Plant - genetics</topic><topic>Glucuronidase - genetics</topic><topic>Glucuronidase - metabolism</topic><topic>Inositol - pharmacology</topic><topic>Intronless</topic><topic>Introns</topic><topic>Isoenzymes - genetics</topic><topic>Lithium - pharmacology</topic><topic>Lycopersicon esculentum</topic><topic>Lycopersicon esculentum - enzymology</topic><topic>Lycopersicon esculentum - genetics</topic><topic>Molecular Sequence Data</topic><topic>Phosphoric Monoester Hydrolases - genetics</topic><topic>Phylogeny</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinant Fusion Proteins - drug effects</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Transgenic plant</topic><topic>β-Glucuronidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Styer, Jean C.</creatorcontrib><creatorcontrib>Keddie, James</creatorcontrib><creatorcontrib>Spence, Jeremiah</creatorcontrib><creatorcontrib>Gillaspy, Glenda E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Styer, Jean C.</au><au>Keddie, James</au><au>Spence, Jeremiah</au><au>Gillaspy, Glenda E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic organization and regulation of the LeIMP-1 and LeIMP-2 genes encoding myo-inositol monophosphatase in tomato</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2004-02-04</date><risdate>2004</risdate><volume>326</volume><spage>35</spage><epage>41</epage><pages>35-41</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Myo-inositol (inositol) monophosphatase (IMP), an enzyme which catalyzes the synthesis of free inositol from various inositol monophosphates, is encoded by a small multigene family in many organisms. The tomato IMP gene family encodes three IMP isoforms with identical in vitro biochemical properties. To determine the role of each tomato
LeIMP gene in plant growth, we isolated the genomic DNA copies of the
LeIMP-1 and
LeIMP-2 genes. The
LeIMP-1 gene spans approximately 5.8 kb and consists of 12 exons, whereas the
LeIMP-2 gene consists of an uninterrupted, single open reading frame (ORF). We have previously shown that steady-state levels of
LeIMP-2 mRNA were very low in comparison to
LeIMP-1 and
LeIMP-3 mRNA levels. To determine whether
LeIMP-2 gene expression was spatially restricted to a discreet domain within the plant we constructed transgenic plants containing an
LeIMP-2 promoter::uidA gene fusion. Analysis of transgenic seedlings revealed that the
LeIMP-2 promoter directed gene expression within epidermal and cortex cells of specific stem/leaf junctions in an abaxial-specific pattern and in the shoot apical meristem. Further, inositol, the product of IMP catalysis, and Li
+, an inhibitor of IMP catalysis, decreased expression of the
LeIMP-2 promoter as measured by a decrease in β-glucuronidase activity after treatment.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>14729261</pmid><doi>10.1016/j.gene.2003.09.048</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals DNA, Plant - chemistry DNA, Plant - genetics Exons Genes, Plant - genetics Glucuronidase - genetics Glucuronidase - metabolism Inositol - pharmacology Intronless Introns Isoenzymes - genetics Lithium - pharmacology Lycopersicon esculentum Lycopersicon esculentum - enzymology Lycopersicon esculentum - genetics Molecular Sequence Data Phosphoric Monoester Hydrolases - genetics Phylogeny Plants, Genetically Modified Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - drug effects Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sequence Analysis, DNA Transgenic plant β-Glucuronidase |
| title | Genomic organization and regulation of the LeIMP-1 and LeIMP-2 genes encoding myo-inositol monophosphatase in tomato |
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