Static and dynamic experiments in cryo‐electron microscopy: comparative observations using high‐vacuum, low‐voltage and low‐vacuum SEM

Static and dynamic experiments in cryo‐electron microscopy: comparative observations using high‐vacuum, low‐voltage and low‐vacuum SEM

Summary We carried out a unique comparative study between three modes of cryo‐scanning electron imaging: high‐vacuum, low‐voltage and low‐vacuum, using ice cream as a model system. Specimens were investigated both with and without a conductive coating (Au/Pd) and at temperatures for which ice either...

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Veröffentlicht in:Journal of microscopy (Oxford) 2004-02, Vol.213 (2), p.198-204
Hauptverfasser: Stokes, D. J., Mugnier, J‐Y., Clarke, C. J.
Format: Artikel
Sprache:eng
Schlagworte:
CryoESEM
cryoSEM
ice cream
in situ sublimation
low vacuum
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Zusammenfassung:Summary We carried out a unique comparative study between three modes of cryo‐scanning electron imaging: high‐vacuum, low‐voltage and low‐vacuum, using ice cream as a model system. Specimens were investigated both with and without a conductive coating (Au/Pd) and at temperatures for which ice either remains fully frozen (
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.2004.01282.x