Median raphe mediates estrogenic effects to the hippocampus in female rats
Subcortical regions such as the medial septum–diagonal band of Broca and supramammillary area have been shown to mediate indirect oestrogenic effects on hippocampal morphology and function. Here, the role of the median raphe (MR), a serotonergic subcortical structure, is studied. To this end, 17β‐es...
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description | Subcortical regions such as the medial septum–diagonal band of Broca and supramammillary area have been shown to mediate indirect oestrogenic effects on hippocampal morphology and function. Here, the role of the median raphe (MR), a serotonergic subcortical structure, is studied. To this end, 17β‐estradiol‐filled 30‐gauge cannulae were implanted into the MR of female ovariectomized rats; cholesterol‐filled cannulae served as controls. After seven days, using unbiased electron microscopic stereological calculations and semiquantitative analysis, the spine synapse density and surface density of glial fibrillary acidic protein‐positive astrocyte processes, respectively, were determined in the stratum radiatum of the CA1 region of the hippocampus. Changes in the serotonergic innervation of the hippocampal CA1 region were determined by immunohistochemistry and subsequent morphometric analysis. In the stratum radiatum of the CA1 region, local estradiol application into the MR resulted in a 47% increase in spine synapse density. Simultaneously, the density of glial fibrillary acidic protein‐positive fibers decreased by 16%. The density of serotonin (5‐HT) innervation of the strata lacunosum moleculare and radiatum of the CA1 region of the hippocampus was reduced in response to estradiol, as shown by a decrease in the length of fibers (27.6 and 48.3% decrease, respectively) and the number of large varicosities (32.5 and 38.8% decrease, respectively). These observations suggest a major role of the MR in mediating oestrogenic effects on the hippocampus and an involvement of the serotonergic system. |
doi_str_mv | 10.1111/j.0953-816X.2003.03124.x |
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Here, the role of the median raphe (MR), a serotonergic subcortical structure, is studied. To this end, 17β‐estradiol‐filled 30‐gauge cannulae were implanted into the MR of female ovariectomized rats; cholesterol‐filled cannulae served as controls. After seven days, using unbiased electron microscopic stereological calculations and semiquantitative analysis, the spine synapse density and surface density of glial fibrillary acidic protein‐positive astrocyte processes, respectively, were determined in the stratum radiatum of the CA1 region of the hippocampus. Changes in the serotonergic innervation of the hippocampal CA1 region were determined by immunohistochemistry and subsequent morphometric analysis. In the stratum radiatum of the CA1 region, local estradiol application into the MR resulted in a 47% increase in spine synapse density. Simultaneously, the density of glial fibrillary acidic protein‐positive fibers decreased by 16%. The density of serotonin (5‐HT) innervation of the strata lacunosum moleculare and radiatum of the CA1 region of the hippocampus was reduced in response to estradiol, as shown by a decrease in the length of fibers (27.6 and 48.3% decrease, respectively) and the number of large varicosities (32.5 and 38.8% decrease, respectively). 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Here, the role of the median raphe (MR), a serotonergic subcortical structure, is studied. To this end, 17β‐estradiol‐filled 30‐gauge cannulae were implanted into the MR of female ovariectomized rats; cholesterol‐filled cannulae served as controls. After seven days, using unbiased electron microscopic stereological calculations and semiquantitative analysis, the spine synapse density and surface density of glial fibrillary acidic protein‐positive astrocyte processes, respectively, were determined in the stratum radiatum of the CA1 region of the hippocampus. Changes in the serotonergic innervation of the hippocampal CA1 region were determined by immunohistochemistry and subsequent morphometric analysis. In the stratum radiatum of the CA1 region, local estradiol application into the MR resulted in a 47% increase in spine synapse density. Simultaneously, the density of glial fibrillary acidic protein‐positive fibers decreased by 16%. The density of serotonin (5‐HT) innervation of the strata lacunosum moleculare and radiatum of the CA1 region of the hippocampus was reduced in response to estradiol, as shown by a decrease in the length of fibers (27.6 and 48.3% decrease, respectively) and the number of large varicosities (32.5 and 38.8% decrease, respectively). These observations suggest a major role of the MR in mediating oestrogenic effects on the hippocampus and an involvement of the serotonergic system.</description><subject>5-HT</subject><subject>Animals</subject><subject>astrocyte processes</subject><subject>Cell Count - methods</subject><subject>Estradiol - pharmacology</subject><subject>Female</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - physiology</subject><subject>Hippocampus - ultrastructure</subject><subject>local estradiol treatment</subject><subject>Ovariectomy</subject><subject>Raphe Nuclei - drug effects</subject><subject>Raphe Nuclei - physiology</subject><subject>Raphe Nuclei - ultrastructure</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>serotonin</subject><subject>spine synapse density</subject><issn>0953-816X</issn><issn>1460-9568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkLtOwzAUQC0EglL4BeSJLcGOH3EGBlTKo-I1gEAslutcQ0rShDgV5e9xaAUjeLEtnXNtHYQwJTEN62gWk0ywSFH5FCeEsJgwmvB4uYEGlEsSZUKqTTT4gXbQrvczQoiSXGyjHcrTRMhEDNDkGvLCzHFrmlfAVX_pwGPwXVu_wLywGJwD23nc1bgLyGvRNLU1VbPwuJhjB5UpIeid30NbzpQe9tf7ED2cje9HF9HV7fnl6OQqspxxHvEs55JL43I2leByngCzQk2Jy6ZK5oILJgCMgpxkkjCucpJaC9QliXRSOjZEh6u5TVu_L8JPdVV4C2Vp5lAvvFaEEpkl9E-QpillnKYBVCvQtrX3LTjdtEVl2k9Nie6D65nuW-q-pe6D6-_gehnUg_Ubi2mo9yuuCwfgeAV8FCV8_nuwHk9u-lPwo5Vf-A6WP75p37RMWSr04825Fnf8OTu9G-ln9gXGV55c</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Prange-Kiel, Janine</creator><creator>Rune, Gabriele M.</creator><creator>Leranth, Csaba</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200401</creationdate><title>Median raphe mediates estrogenic effects to the hippocampus in female rats</title><author>Prange-Kiel, Janine ; Rune, Gabriele M. ; Leranth, Csaba</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4344-49d4646afd3b6efd42e3c58b0f9b86d54535eea8ed0960348d07cce1f226f66f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>5-HT</topic><topic>Animals</topic><topic>astrocyte processes</topic><topic>Cell Count - methods</topic><topic>Estradiol - pharmacology</topic><topic>Female</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - physiology</topic><topic>Hippocampus - ultrastructure</topic><topic>local estradiol treatment</topic><topic>Ovariectomy</topic><topic>Raphe Nuclei - drug effects</topic><topic>Raphe Nuclei - physiology</topic><topic>Raphe Nuclei - ultrastructure</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>serotonin</topic><topic>spine synapse density</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prange-Kiel, Janine</creatorcontrib><creatorcontrib>Rune, Gabriele M.</creatorcontrib><creatorcontrib>Leranth, Csaba</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The European journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prange-Kiel, Janine</au><au>Rune, Gabriele M.</au><au>Leranth, Csaba</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Median raphe mediates estrogenic effects to the hippocampus in female rats</atitle><jtitle>The European journal of neuroscience</jtitle><addtitle>Eur J Neurosci</addtitle><date>2004-01</date><risdate>2004</risdate><volume>19</volume><issue>2</issue><spage>309</spage><epage>317</epage><pages>309-317</pages><issn>0953-816X</issn><eissn>1460-9568</eissn><abstract>Subcortical regions such as the medial septum–diagonal band of Broca and supramammillary area have been shown to mediate indirect oestrogenic effects on hippocampal morphology and function. Here, the role of the median raphe (MR), a serotonergic subcortical structure, is studied. To this end, 17β‐estradiol‐filled 30‐gauge cannulae were implanted into the MR of female ovariectomized rats; cholesterol‐filled cannulae served as controls. After seven days, using unbiased electron microscopic stereological calculations and semiquantitative analysis, the spine synapse density and surface density of glial fibrillary acidic protein‐positive astrocyte processes, respectively, were determined in the stratum radiatum of the CA1 region of the hippocampus. Changes in the serotonergic innervation of the hippocampal CA1 region were determined by immunohistochemistry and subsequent morphometric analysis. In the stratum radiatum of the CA1 region, local estradiol application into the MR resulted in a 47% increase in spine synapse density. Simultaneously, the density of glial fibrillary acidic protein‐positive fibers decreased by 16%. The density of serotonin (5‐HT) innervation of the strata lacunosum moleculare and radiatum of the CA1 region of the hippocampus was reduced in response to estradiol, as shown by a decrease in the length of fibers (27.6 and 48.3% decrease, respectively) and the number of large varicosities (32.5 and 38.8% decrease, respectively). These observations suggest a major role of the MR in mediating oestrogenic effects on the hippocampus and an involvement of the serotonergic system.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>14725625</pmid><doi>10.1111/j.0953-816X.2003.03124.x</doi><tpages>9</tpages></addata></record> |
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subjects | 5-HT Animals astrocyte processes Cell Count - methods Estradiol - pharmacology Female Hippocampus - drug effects Hippocampus - physiology Hippocampus - ultrastructure local estradiol treatment Ovariectomy Raphe Nuclei - drug effects Raphe Nuclei - physiology Raphe Nuclei - ultrastructure Rats Rats, Wistar serotonin spine synapse density |
title | Median raphe mediates estrogenic effects to the hippocampus in female rats |
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