Bacterial translocation, endotoxaemia and apoptosis following Pringle manoeuvre in rats
Background: Intraoperative occlusion of the hepatoduodenal ligament (Pringle manoeuvre (Pm)) is often employed for the reduction of blood loss during liver surgery. No data exist to date on the effects of Pm on mucosal barrier dysfunction, systemic bacterial translocation (BT), endotoxaemia and apop...
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| Veröffentlicht in: | Injury 2004, Vol.35 (1), p.35-43 |
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| creator | Filos, Kriton S Kirkilesis, Ioannis Spiliopoulou, Iris Scopa, Chrisoula D Nikolopoulou, Vassiliki Kouraklis, Gregory Vagianos, Constantine E |
| description | Background: Intraoperative occlusion of the hepatoduodenal ligament (Pringle manoeuvre (Pm)) is often employed for the reduction of blood loss during liver surgery. No data exist to date on the effects of Pm on mucosal barrier dysfunction, systemic bacterial translocation (BT), endotoxaemia and apoptosis.
Materials and methods: Sixty-five male Wistar rats in three groups: I (
n=25) controls, II (
n=20) sham operation, III (
n=20) occlusion of the hepatoduodenal ligament (Pm). Tissue samples from mesenteric lymph nodes (MLNs), liver, lungs and spleen were analysed after 30
min and at 24
h. Endotoxin was measured in portal and aortic blood and routine haematological and biochemical parameters were measured before and after Pm.
Results: No differences were found in the blood parameters before and after Pm, but a significant increase in contaminated MLNs and liver was noted. All cultured bacteria were enteric in origin. Portal and aortic endotoxin were significantly increased. Overall the ileal architecture remained intact in all specimens studied and no significant pathology was observed. The ABC increased after Pm significantly (
P |
| doi_str_mv | 10.1016/S0020-1383(03)00288-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80106494</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0020138303002882</els_id><sourcerecordid>80106494</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-df8590c2e2f463f3dbd5f83a0baff6d528d2c2894ec53029470950ff31d7f01c3</originalsourceid><addsrcrecordid>eNqFkFtrVDEQgIModlv9CUpelAoenSTnkvMkWrQVCgoqPoZsMpFITrJNsq3-e7Pu0j4WhhkGvrnwEfKMwRsGbHz7DYBDx4QUpyBetUbKjj8gKyanuQM-Tg_J6hY5Isel_AZgEwjxmByxfuJyHsSK_PygTcXsdaA161hCMrr6FF9TjDbV9Efj4jXV0VK9SZuaii_UpRDSjY-_6NfcckC66Jhwe52R-kizruUJeeR0KPj0UE_Ij08fv59ddJdfzj-fvb_sTM957ayTwwyGI3f9KJywazs4KTSstXOjHbi03LRXezSDAD73E8wDOCeYnRwwI07Iy_3eTU5XWyxVLb4YDEFHTNuiJDAY-7m_F-TNVTsBDRz2oMmplIxObbJfdP6rGKidevVfvdp5VdBip17xNvf8cGC7XtDeTR1cN-DFAdDF6OCabuPLHTcIOTK5W_Ruz2Hzdu0xq2I8RoPWZzRV2eTveeUfu9igxQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20205300</pqid></control><display><type>article</type><title>Bacterial translocation, endotoxaemia and apoptosis following Pringle manoeuvre in rats</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Filos, Kriton S ; Kirkilesis, Ioannis ; Spiliopoulou, Iris ; Scopa, Chrisoula D ; Nikolopoulou, Vassiliki ; Kouraklis, Gregory ; Vagianos, Constantine E</creator><creatorcontrib>Filos, Kriton S ; Kirkilesis, Ioannis ; Spiliopoulou, Iris ; Scopa, Chrisoula D ; Nikolopoulou, Vassiliki ; Kouraklis, Gregory ; Vagianos, Constantine E</creatorcontrib><description>Background: Intraoperative occlusion of the hepatoduodenal ligament (Pringle manoeuvre (Pm)) is often employed for the reduction of blood loss during liver surgery. No data exist to date on the effects of Pm on mucosal barrier dysfunction, systemic bacterial translocation (BT), endotoxaemia and apoptosis.
Materials and methods: Sixty-five male Wistar rats in three groups: I (
n=25) controls, II (
n=20) sham operation, III (
n=20) occlusion of the hepatoduodenal ligament (Pm). Tissue samples from mesenteric lymph nodes (MLNs), liver, lungs and spleen were analysed after 30
min and at 24
h. Endotoxin was measured in portal and aortic blood and routine haematological and biochemical parameters were measured before and after Pm.
Results: No differences were found in the blood parameters before and after Pm, but a significant increase in contaminated MLNs and liver was noted. All cultured bacteria were enteric in origin. Portal and aortic endotoxin were significantly increased. Overall the ileal architecture remained intact in all specimens studied and no significant pathology was observed. The ABC increased after Pm significantly (
P<0.01).
Conclusion: Normothermic Pm of 30
min duration results in immediate and delayed gut barrier failure by significantly increasing BT and endotoxaemia which might be attributed to portal stasis leading to intestinal congestion as well as temporary liver ischaemia. Apoptosis increased significantly 30
min after performing the Pm.</description><identifier>ISSN: 0020-1383</identifier><identifier>EISSN: 1879-0267</identifier><identifier>DOI: 10.1016/S0020-1383(03)00288-2</identifier><identifier>PMID: 14728953</identifier><identifier>CODEN: INJUBF</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Apoptosis ; Bacteria ; Bacterial Translocation ; Bile - microbiology ; Biological and medical sciences ; Cecum - microbiology ; Constriction ; Culture Techniques ; Disease Models, Animal ; Diseases of the osteoarticular system ; Endotoxemia - etiology ; Endotoxin ; Hemostasis, Surgical - adverse effects ; Ileum - pathology ; Intestinal Mucosa - pathology ; Liver - microbiology ; Lymph Nodes - microbiology ; Male ; Medical sciences ; Mesentery ; Pringle manoeuvre ; Rats ; Rats, Wistar ; Trauma ; Traumas. Diseases due to physical agents</subject><ispartof>Injury, 2004, Vol.35 (1), p.35-43</ispartof><rights>2003 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-df8590c2e2f463f3dbd5f83a0baff6d528d2c2894ec53029470950ff31d7f01c3</citedby><cites>FETCH-LOGICAL-c422t-df8590c2e2f463f3dbd5f83a0baff6d528d2c2894ec53029470950ff31d7f01c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0020138303002882$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15386182$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14728953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filos, Kriton S</creatorcontrib><creatorcontrib>Kirkilesis, Ioannis</creatorcontrib><creatorcontrib>Spiliopoulou, Iris</creatorcontrib><creatorcontrib>Scopa, Chrisoula D</creatorcontrib><creatorcontrib>Nikolopoulou, Vassiliki</creatorcontrib><creatorcontrib>Kouraklis, Gregory</creatorcontrib><creatorcontrib>Vagianos, Constantine E</creatorcontrib><title>Bacterial translocation, endotoxaemia and apoptosis following Pringle manoeuvre in rats</title><title>Injury</title><addtitle>Injury</addtitle><description>Background: Intraoperative occlusion of the hepatoduodenal ligament (Pringle manoeuvre (Pm)) is often employed for the reduction of blood loss during liver surgery. No data exist to date on the effects of Pm on mucosal barrier dysfunction, systemic bacterial translocation (BT), endotoxaemia and apoptosis.
Materials and methods: Sixty-five male Wistar rats in three groups: I (
n=25) controls, II (
n=20) sham operation, III (
n=20) occlusion of the hepatoduodenal ligament (Pm). Tissue samples from mesenteric lymph nodes (MLNs), liver, lungs and spleen were analysed after 30
min and at 24
h. Endotoxin was measured in portal and aortic blood and routine haematological and biochemical parameters were measured before and after Pm.
Results: No differences were found in the blood parameters before and after Pm, but a significant increase in contaminated MLNs and liver was noted. All cultured bacteria were enteric in origin. Portal and aortic endotoxin were significantly increased. Overall the ileal architecture remained intact in all specimens studied and no significant pathology was observed. The ABC increased after Pm significantly (
P<0.01).
Conclusion: Normothermic Pm of 30
min duration results in immediate and delayed gut barrier failure by significantly increasing BT and endotoxaemia which might be attributed to portal stasis leading to intestinal congestion as well as temporary liver ischaemia. Apoptosis increased significantly 30
min after performing the Pm.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Bacteria</subject><subject>Bacterial Translocation</subject><subject>Bile - microbiology</subject><subject>Biological and medical sciences</subject><subject>Cecum - microbiology</subject><subject>Constriction</subject><subject>Culture Techniques</subject><subject>Disease Models, Animal</subject><subject>Diseases of the osteoarticular system</subject><subject>Endotoxemia - etiology</subject><subject>Endotoxin</subject><subject>Hemostasis, Surgical - adverse effects</subject><subject>Ileum - pathology</subject><subject>Intestinal Mucosa - pathology</subject><subject>Liver - microbiology</subject><subject>Lymph Nodes - microbiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mesentery</subject><subject>Pringle manoeuvre</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Trauma</subject><subject>Traumas. Diseases due to physical agents</subject><issn>0020-1383</issn><issn>1879-0267</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFtrVDEQgIModlv9CUpelAoenSTnkvMkWrQVCgoqPoZsMpFITrJNsq3-e7Pu0j4WhhkGvrnwEfKMwRsGbHz7DYBDx4QUpyBetUbKjj8gKyanuQM-Tg_J6hY5Isel_AZgEwjxmByxfuJyHsSK_PygTcXsdaA161hCMrr6FF9TjDbV9Efj4jXV0VK9SZuaii_UpRDSjY-_6NfcckC66Jhwe52R-kizruUJeeR0KPj0UE_Ij08fv59ddJdfzj-fvb_sTM957ayTwwyGI3f9KJywazs4KTSstXOjHbi03LRXezSDAD73E8wDOCeYnRwwI07Iy_3eTU5XWyxVLb4YDEFHTNuiJDAY-7m_F-TNVTsBDRz2oMmplIxObbJfdP6rGKidevVfvdp5VdBip17xNvf8cGC7XtDeTR1cN-DFAdDF6OCabuPLHTcIOTK5W_Ruz2Hzdu0xq2I8RoPWZzRV2eTveeUfu9igxQ</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Filos, Kriton S</creator><creator>Kirkilesis, Ioannis</creator><creator>Spiliopoulou, Iris</creator><creator>Scopa, Chrisoula D</creator><creator>Nikolopoulou, Vassiliki</creator><creator>Kouraklis, Gregory</creator><creator>Vagianos, Constantine E</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>Bacterial translocation, endotoxaemia and apoptosis following Pringle manoeuvre in rats</title><author>Filos, Kriton S ; Kirkilesis, Ioannis ; Spiliopoulou, Iris ; Scopa, Chrisoula D ; Nikolopoulou, Vassiliki ; Kouraklis, Gregory ; Vagianos, Constantine E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-df8590c2e2f463f3dbd5f83a0baff6d528d2c2894ec53029470950ff31d7f01c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Bacteria</topic><topic>Bacterial Translocation</topic><topic>Bile - microbiology</topic><topic>Biological and medical sciences</topic><topic>Cecum - microbiology</topic><topic>Constriction</topic><topic>Culture Techniques</topic><topic>Disease Models, Animal</topic><topic>Diseases of the osteoarticular system</topic><topic>Endotoxemia - etiology</topic><topic>Endotoxin</topic><topic>Hemostasis, Surgical - adverse effects</topic><topic>Ileum - pathology</topic><topic>Intestinal Mucosa - pathology</topic><topic>Liver - microbiology</topic><topic>Lymph Nodes - microbiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mesentery</topic><topic>Pringle manoeuvre</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Trauma</topic><topic>Traumas. Diseases due to physical agents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Filos, Kriton S</creatorcontrib><creatorcontrib>Kirkilesis, Ioannis</creatorcontrib><creatorcontrib>Spiliopoulou, Iris</creatorcontrib><creatorcontrib>Scopa, Chrisoula D</creatorcontrib><creatorcontrib>Nikolopoulou, Vassiliki</creatorcontrib><creatorcontrib>Kouraklis, Gregory</creatorcontrib><creatorcontrib>Vagianos, Constantine E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Injury</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filos, Kriton S</au><au>Kirkilesis, Ioannis</au><au>Spiliopoulou, Iris</au><au>Scopa, Chrisoula D</au><au>Nikolopoulou, Vassiliki</au><au>Kouraklis, Gregory</au><au>Vagianos, Constantine E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial translocation, endotoxaemia and apoptosis following Pringle manoeuvre in rats</atitle><jtitle>Injury</jtitle><addtitle>Injury</addtitle><date>2004</date><risdate>2004</risdate><volume>35</volume><issue>1</issue><spage>35</spage><epage>43</epage><pages>35-43</pages><issn>0020-1383</issn><eissn>1879-0267</eissn><coden>INJUBF</coden><abstract>Background: Intraoperative occlusion of the hepatoduodenal ligament (Pringle manoeuvre (Pm)) is often employed for the reduction of blood loss during liver surgery. No data exist to date on the effects of Pm on mucosal barrier dysfunction, systemic bacterial translocation (BT), endotoxaemia and apoptosis.
Materials and methods: Sixty-five male Wistar rats in three groups: I (
n=25) controls, II (
n=20) sham operation, III (
n=20) occlusion of the hepatoduodenal ligament (Pm). Tissue samples from mesenteric lymph nodes (MLNs), liver, lungs and spleen were analysed after 30
min and at 24
h. Endotoxin was measured in portal and aortic blood and routine haematological and biochemical parameters were measured before and after Pm.
Results: No differences were found in the blood parameters before and after Pm, but a significant increase in contaminated MLNs and liver was noted. All cultured bacteria were enteric in origin. Portal and aortic endotoxin were significantly increased. Overall the ileal architecture remained intact in all specimens studied and no significant pathology was observed. The ABC increased after Pm significantly (
P<0.01).
Conclusion: Normothermic Pm of 30
min duration results in immediate and delayed gut barrier failure by significantly increasing BT and endotoxaemia which might be attributed to portal stasis leading to intestinal congestion as well as temporary liver ischaemia. Apoptosis increased significantly 30
min after performing the Pm.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>14728953</pmid><doi>10.1016/S0020-1383(03)00288-2</doi><tpages>9</tpages></addata></record> |
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| ispartof | Injury, 2004, Vol.35 (1), p.35-43 |
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| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Apoptosis Bacteria Bacterial Translocation Bile - microbiology Biological and medical sciences Cecum - microbiology Constriction Culture Techniques Disease Models, Animal Diseases of the osteoarticular system Endotoxemia - etiology Endotoxin Hemostasis, Surgical - adverse effects Ileum - pathology Intestinal Mucosa - pathology Liver - microbiology Lymph Nodes - microbiology Male Medical sciences Mesentery Pringle manoeuvre Rats Rats, Wistar Trauma Traumas. Diseases due to physical agents |
| title | Bacterial translocation, endotoxaemia and apoptosis following Pringle manoeuvre in rats |
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