A Recoverable Enzymatic Microgel Based on Biomolecular Recognition

The enzyme β-galactosidase has been immobilized through incorporation into a selectively soluble microgel, prepared from DNA, biotinylated peptide nucleic acid (PNA), and the protein avidin. The enzyme was conjugated to avidin, allowing it to be integrated directly into the microgel network. Efficie...

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Veröffentlicht in:	Journal of the American Chemical Society 2004-01, Vol.126 (3), p.726-727
Hauptverfasser:	Cao, Rong, Gu, Zhenyu, Patterson, Gary D, Armitage, Bruce A
Format:	Artikel
Sprache:	eng
Schlagworte:	Avidin - chemistry beta-Galactosidase - chemistry beta-Galactosidase - metabolism Biological and medical sciences Biotechnology Biotin - chemistry DNA - chemistry Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Fundamental and applied biological sciences. Psychology Gels Immobilization of enzymes and other molecules Immobilization techniques Kinetics Methods. Procedures. Technologies Nitrophenylgalactosides - chemistry Nitrophenylgalactosides - metabolism Nucleic Acid Conformation
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