Neural stem and progenitor cells in nestin-GFP transgenic mice

Neural stem cells generate a wide spectrum of cell types in developing and adult nervous systems. These cells are marked by expression of the intermediate filament nestin. We used the regulatory elements of the nestin gene to generate transgenic mice in which neural stem cells of the embryonic and a...

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Veröffentlicht in:	Journal of comparative neurology (1911) 2004-02, Vol.469 (3), p.311-324
Hauptverfasser:	Mignone, John L., Kukekov, Valery, Chiang, Ann-Shyn, Steindler, Dennis, Enikolopov, Grigori
Format:	Artikel
Sprache:	eng
Schlagworte:	adult neurogenesis Animals Brain - cytology Brain - growth & development Brain - metabolism bromodeoxyuridine Bromodeoxyuridine - metabolism Cell Count Cells, Cultured doublecortin Embryo, Mammalian Flow Cytometry - methods fluorescence activated flow cytometry Gene Expression Regulation, Developmental glial fibrillary acidic protein Glial Fibrillary Acidic Protein - metabolism green fluorescent protein Green Fluorescent Proteins hippocampus Hippocampus - cytology Immunohistochemistry Indicators and Reagents Intermediate Filament Proteins - genetics Intermediate Filament Proteins - metabolism Luminescent Proteins - genetics Luminescent Proteins - metabolism Mice Mice, Transgenic Microscopy, Confocal Nerve Tissue Proteins Nestin Neurons - metabolism neurosphere Proteins - metabolism Receptor-Interacting Protein Serine-Threonine Kinases Reverse Transcriptase Polymerase Chain Reaction - methods Stem Cell Transplantation stem cells Stem Cells - metabolism Time Factors Tubulin - metabolism
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