[27] Assays for cytochrome P-450 peroxygenase activity

This chapter discusses assays for cytochrome P-450 peroxygenase activity. Toluene, which yields benzyl alcohol in the cytochrome P-450-catalyzed peroxygenase reaction, is a useful substrate in mechanistic studies. To determine benzyl alcohol formation at fixed time intervals, a gas chromatographic a...

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Veröffentlicht in:	Methods in Enzymology 1990, Vol.186, p.273-278
Hauptverfasser:	Coon, Minor J., Blake, Robert C., White, Ronald E., Nordblom, Gerald D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Dehydrogenase Analytical, structural and metabolic biochemistry Aniline Compounds Animals Benzene Derivatives Biological and medical sciences Carbohydrates Cytochrome P-450 Enzyme System - analysis Cytochrome P-450 Enzyme System - metabolism Free Radicals Fundamental and applied biological sciences. Psychology Holosides Indicators and Reagents Microsomes, Liver - metabolism Other biological molecules Peroxidases - analysis Peroxidases - metabolism Spectrophotometry - methods
Online-Zugang:	Volltext
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