Kinetoplast DNA minicircles are inherited from both parents in genetic hybrids of Trypanosoma brucei
We have examined the inheritance of kinetoplast DNA (kDNA) in genetic crosses of trypanosomes. In 2 independent crosses of Trypanosoma brucei spp. trypanosomes, the kDNA maxicircles which carry the genes for mitochondrial biogenesis, were inherited from one parent only, as already found by other wor...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1990-08, Vol.42 (1), p.45-53 |
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| creator | Gibson, Wendy Garside, Lisa |
| description | We have examined the inheritance of kinetoplast DNA (kDNA) in genetic crosses of trypanosomes. In 2 independent crosses of
Trypanosoma brucei spp. trypanosomes, the kDNA maxicircles which carry the genes for mitochondrial biogenesis, were inherited from one parent only, as already found by other workers. However, the other component of kDNA, the minicircles, were inherited from both parents. This was demonstrated by Southern analysis using cloned minicircle probes. The inheritance of kDNA is therefore not uniparental. Our data point to fusion of the parental kinetoplast DNA networks during genetic exchange, with gradual loss of one or other parental maxicircle type due to random segregation of maxicircles at subsequent mitotic divisions. We infer that the first event of genetic exchange is fusion of parental trypanosomes (either haploid or diploid), followed at some point by fusion of the parental mitochondria. |
| doi_str_mv | 10.1016/0166-6851(90)90111-X |
| format | Article |
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Trypanosoma brucei spp. trypanosomes, the kDNA maxicircles which carry the genes for mitochondrial biogenesis, were inherited from one parent only, as already found by other workers. However, the other component of kDNA, the minicircles, were inherited from both parents. This was demonstrated by Southern analysis using cloned minicircle probes. The inheritance of kDNA is therefore not uniparental. Our data point to fusion of the parental kinetoplast DNA networks during genetic exchange, with gradual loss of one or other parental maxicircle type due to random segregation of maxicircles at subsequent mitotic divisions. We infer that the first event of genetic exchange is fusion of parental trypanosomes (either haploid or diploid), followed at some point by fusion of the parental mitochondria.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Crosses, Genetic</subject><subject>DNA, Circular - metabolism</subject><subject>DNA, Mitochondrial - metabolism</subject><subject>DNA, Protozoan - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic exchange</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Kinetoplast DNA</subject><subject>Mitochondrial DNA</subject><subject>Nucleic Acid Hybridization</subject><subject>Polymorphism, Genetic</subject><subject>Protozoa</subject><subject>Trypanosoma brucei</subject><subject>Trypanosoma brucei brucei - genetics</subject><subject>Trypanosome</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFP2zAYhi0EKoXtHzDJF6btEPbZSRz7MqnqGCAQuzCpN8txvqxGSZzZ6aT-e9y1ghsH6zu8z_vKegi5YHDFgIlv6YlMyJJ9UfBVAWMsWx2ROZMVz1TB5TGZvyKn5CzGZwAoKyFmZMZ5nkul5qS5dwNOfuxMnOiPxwXt3eCsC7bDSE1A6oY1BjdhQ9vge1r7aU3HFAxTTBn9g6nuLF1v6-CaSH1Ln8J2NIOPvje0DhuL7gM5aU0X8ePhnpPfP6-flrfZw6-bu-XiIbO5rKbMFqUUFZQgbNWyxvAWAJWBulSsLhBsyWuTc1YK20LJKpBFARwrDjXPIUXn5PN-dwz-7wbjpHsXLXadGdBvopYASopcJLDYgzb4GAO2egyuN2GrGeidXL0zp3fmtAL9X65epdqnw_6m7rF5LR1spvzykJtoTdcGM1gX37aV4Em_TNz3PYdJxj-HQUfrcLDYuIB20o1373_kBd0XlfU</recordid><startdate>19900801</startdate><enddate>19900801</enddate><creator>Gibson, Wendy</creator><creator>Garside, Lisa</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900801</creationdate><title>Kinetoplast DNA minicircles are inherited from both parents in genetic hybrids of Trypanosoma brucei</title><author>Gibson, Wendy ; Garside, Lisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-c458670506c7f1da2f00e9a0b591b4e0c52ba32156cf0517084402e720b230ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Crosses, Genetic</topic><topic>DNA, Circular - metabolism</topic><topic>DNA, Mitochondrial - metabolism</topic><topic>DNA, Protozoan - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic exchange</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Kinetoplast DNA</topic><topic>Mitochondrial DNA</topic><topic>Nucleic Acid Hybridization</topic><topic>Polymorphism, Genetic</topic><topic>Protozoa</topic><topic>Trypanosoma brucei</topic><topic>Trypanosoma brucei brucei - genetics</topic><topic>Trypanosome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gibson, Wendy</creatorcontrib><creatorcontrib>Garside, Lisa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gibson, Wendy</au><au>Garside, Lisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetoplast DNA minicircles are inherited from both parents in genetic hybrids of Trypanosoma brucei</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1990-08-01</date><risdate>1990</risdate><volume>42</volume><issue>1</issue><spage>45</spage><epage>53</epage><pages>45-53</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><coden>MBIPDP</coden><abstract>We have examined the inheritance of kinetoplast DNA (kDNA) in genetic crosses of trypanosomes. 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Trypanosoma brucei spp. trypanosomes, the kDNA maxicircles which carry the genes for mitochondrial biogenesis, were inherited from one parent only, as already found by other workers. However, the other component of kDNA, the minicircles, were inherited from both parents. This was demonstrated by Southern analysis using cloned minicircle probes. The inheritance of kDNA is therefore not uniparental. Our data point to fusion of the parental kinetoplast DNA networks during genetic exchange, with gradual loss of one or other parental maxicircle type due to random segregation of maxicircles at subsequent mitotic divisions. We infer that the first event of genetic exchange is fusion of parental trypanosomes (either haploid or diploid), followed at some point by fusion of the parental mitochondria.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>2233899</pmid><doi>10.1016/0166-6851(90)90111-X</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular and biochemical parasitology, 1990-08, Vol.42 (1), p.45-53 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Classical genetics, quantitative genetics, hybrids Crosses, Genetic DNA, Circular - metabolism DNA, Mitochondrial - metabolism DNA, Protozoan - metabolism Fundamental and applied biological sciences. Psychology Genetic exchange Genetics of eukaryotes. Biological and molecular evolution Kinetoplast DNA Mitochondrial DNA Nucleic Acid Hybridization Polymorphism, Genetic Protozoa Trypanosoma brucei Trypanosoma brucei brucei - genetics Trypanosome |
| title | Kinetoplast DNA minicircles are inherited from both parents in genetic hybrids of Trypanosoma brucei |
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