Osmotic response in Lactobacillus casei ATCC 393: biochemical and biophysical characteristics of membrane
The biochemical and biophysical properties of the membrane and some general characteristics of the response of Lactobacillus casei ATCC 393 (reclassified Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 2004-02, Vol.422 (1), p.61-70 |
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| creator | Machado, M.Cecilia López, Claudia S Heras, Horacio Rivas, Emilio A |
| description | The biochemical and biophysical properties of the membrane and some general characteristics of the response of
Lactobacillus casei ATCC 393 (reclassified
Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the cultures were increased. The glycolipid AcylH3DG is only present in membranes of NaCl containing medium, whereas, H4DG undergoes a significant increment and H2DG a significant decrease. The fluidity of both the purified membranes and the total lipid vesicles, as determined with the fluorescent probe DPH, did not change in conditions of high salinity. This was coincident with changes in the fatty acid (FA) composition where an increase in the saturated/unsaturated FA ratio was compensated by a rise in the fluidifying 11,12-methyleneoctadecanoic FA (cyc 19:0). Under osmotic stress conditions, Laurdan and acridine orange in total lipid vesicles showed increased lateral lipid packing and proton permeability, respectively. |
| doi_str_mv | 10.1016/j.abb.2003.11.001 |
| format | Article |
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Lactobacillus casei ATCC 393 (reclassified
Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the cultures were increased. The glycolipid AcylH3DG is only present in membranes of NaCl containing medium, whereas, H4DG undergoes a significant increment and H2DG a significant decrease. The fluidity of both the purified membranes and the total lipid vesicles, as determined with the fluorescent probe DPH, did not change in conditions of high salinity. This was coincident with changes in the fatty acid (FA) composition where an increase in the saturated/unsaturated FA ratio was compensated by a rise in the fluidifying 11,12-methyleneoctadecanoic FA (cyc 19:0). Under osmotic stress conditions, Laurdan and acridine orange in total lipid vesicles showed increased lateral lipid packing and proton permeability, respectively.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/j.abb.2003.11.001</identifier><identifier>PMID: 14725858</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bile Acids and Salts - pharmacology ; Bile salts ; Cell Membrane - chemistry ; Cell Membrane - physiology ; Cell Membrane Permeability ; Culture Media ; Diphenylhexatriene - chemistry ; Fatty Acids - analysis ; Fatty Acids - chemistry ; Fluorescence Polarization ; Fluorescent probes ; Glycolipids - analysis ; Hydrophobic and Hydrophilic Interactions ; Lactobacillus casei ; Lactobacillus casei - chemistry ; Lactobacillus casei - genetics ; Lactobacillus casei - physiology ; Lipids ; Liposomes - chemistry ; Membrane Fluidity ; Membrane Lipids - analysis ; Membranes ; Osmotic Pressure ; Osmotic stress ; Permeability ; Phospholipids - analysis ; Protons ; Saline Solution, Hypertonic - pharmacology</subject><ispartof>Archives of biochemistry and biophysics, 2004-02, Vol.422 (1), p.61-70</ispartof><rights>2003 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-9f0cf589c2c0cd0993aff3f46a95a79ee48e47e06d9b7cc3402729abd8a16c1e3</citedby><cites>FETCH-LOGICAL-c415t-9f0cf589c2c0cd0993aff3f46a95a79ee48e47e06d9b7cc3402729abd8a16c1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.abb.2003.11.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14725858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Machado, M.Cecilia</creatorcontrib><creatorcontrib>López, Claudia S</creatorcontrib><creatorcontrib>Heras, Horacio</creatorcontrib><creatorcontrib>Rivas, Emilio A</creatorcontrib><title>Osmotic response in Lactobacillus casei ATCC 393: biochemical and biophysical characteristics of membrane</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The biochemical and biophysical properties of the membrane and some general characteristics of the response of
Lactobacillus casei ATCC 393 (reclassified
Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the cultures were increased. The glycolipid AcylH3DG is only present in membranes of NaCl containing medium, whereas, H4DG undergoes a significant increment and H2DG a significant decrease. The fluidity of both the purified membranes and the total lipid vesicles, as determined with the fluorescent probe DPH, did not change in conditions of high salinity. This was coincident with changes in the fatty acid (FA) composition where an increase in the saturated/unsaturated FA ratio was compensated by a rise in the fluidifying 11,12-methyleneoctadecanoic FA (cyc 19:0). Under osmotic stress conditions, Laurdan and acridine orange in total lipid vesicles showed increased lateral lipid packing and proton permeability, respectively.</description><subject>Bile Acids and Salts - pharmacology</subject><subject>Bile salts</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - physiology</subject><subject>Cell Membrane Permeability</subject><subject>Culture Media</subject><subject>Diphenylhexatriene - chemistry</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - chemistry</subject><subject>Fluorescence Polarization</subject><subject>Fluorescent probes</subject><subject>Glycolipids - analysis</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Lactobacillus casei</subject><subject>Lactobacillus casei - chemistry</subject><subject>Lactobacillus casei - genetics</subject><subject>Lactobacillus casei - physiology</subject><subject>Lipids</subject><subject>Liposomes - chemistry</subject><subject>Membrane Fluidity</subject><subject>Membrane Lipids - analysis</subject><subject>Membranes</subject><subject>Osmotic Pressure</subject><subject>Osmotic stress</subject><subject>Permeability</subject><subject>Phospholipids - analysis</subject><subject>Protons</subject><subject>Saline Solution, Hypertonic - pharmacology</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2P0zAQhi0EYrsLP4AL8olbwky-DadVBQtSpb0sZ8uZTFRXSVw8KdL--3VpJW6crJGf99XMo9QHhBwBm8-H3PV9XgCUOWIOgK_UBsE0GZRd9VptIP1kpmvwRt2KHBKAVVO8VTdYtUXd1d1G-UeZw-pJR5ZjWIS1X_TO0Rp6R36aTqLJCXt9_7Td6tKUX3TvA-159uQm7ZbhPB_3z_J3pr2LKczRSyoVHUY989xHt_A79WZ0k_D763unfn3_9rT9ke0eH35u73cZVVivmRmBxrozVBDQAMaUbhzLsWqcqV1rmKuOq5ahGUzfEpUVFG1hXD90DhtCLu_Up0vvMYbfJ5bVzl6IpyntEE5iOwDTmhYTiBeQYhCJPNpj9LOLzxbBnv3ag01-7dmvRbRJX8p8vJaf-pmHf4mr0AR8vQCcTvzjOVohzwvx4CPTaofg_1P_Al3Di8s</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Machado, M.Cecilia</creator><creator>López, Claudia S</creator><creator>Heras, Horacio</creator><creator>Rivas, Emilio A</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040201</creationdate><title>Osmotic response in Lactobacillus casei ATCC 393: biochemical and biophysical characteristics of membrane</title><author>Machado, M.Cecilia ; López, Claudia S ; Heras, Horacio ; Rivas, Emilio A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-9f0cf589c2c0cd0993aff3f46a95a79ee48e47e06d9b7cc3402729abd8a16c1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Bile Acids and Salts - pharmacology</topic><topic>Bile salts</topic><topic>Cell Membrane - chemistry</topic><topic>Cell Membrane - physiology</topic><topic>Cell Membrane Permeability</topic><topic>Culture Media</topic><topic>Diphenylhexatriene - chemistry</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - chemistry</topic><topic>Fluorescence Polarization</topic><topic>Fluorescent probes</topic><topic>Glycolipids - analysis</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Lactobacillus casei</topic><topic>Lactobacillus casei - chemistry</topic><topic>Lactobacillus casei - genetics</topic><topic>Lactobacillus casei - physiology</topic><topic>Lipids</topic><topic>Liposomes - chemistry</topic><topic>Membrane Fluidity</topic><topic>Membrane Lipids - analysis</topic><topic>Membranes</topic><topic>Osmotic Pressure</topic><topic>Osmotic stress</topic><topic>Permeability</topic><topic>Phospholipids - analysis</topic><topic>Protons</topic><topic>Saline Solution, Hypertonic - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Machado, M.Cecilia</creatorcontrib><creatorcontrib>López, Claudia S</creatorcontrib><creatorcontrib>Heras, Horacio</creatorcontrib><creatorcontrib>Rivas, Emilio A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Machado, M.Cecilia</au><au>López, Claudia S</au><au>Heras, Horacio</au><au>Rivas, Emilio A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osmotic response in Lactobacillus casei ATCC 393: biochemical and biophysical characteristics of membrane</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>2004-02-01</date><risdate>2004</risdate><volume>422</volume><issue>1</issue><spage>61</spage><epage>70</epage><pages>61-70</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The biochemical and biophysical properties of the membrane and some general characteristics of the response of
Lactobacillus casei ATCC 393 (reclassified
Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the cultures were increased. The glycolipid AcylH3DG is only present in membranes of NaCl containing medium, whereas, H4DG undergoes a significant increment and H2DG a significant decrease. The fluidity of both the purified membranes and the total lipid vesicles, as determined with the fluorescent probe DPH, did not change in conditions of high salinity. This was coincident with changes in the fatty acid (FA) composition where an increase in the saturated/unsaturated FA ratio was compensated by a rise in the fluidifying 11,12-methyleneoctadecanoic FA (cyc 19:0). Under osmotic stress conditions, Laurdan and acridine orange in total lipid vesicles showed increased lateral lipid packing and proton permeability, respectively.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>14725858</pmid><doi>10.1016/j.abb.2003.11.001</doi><tpages>10</tpages></addata></record> |
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| subjects | Bile Acids and Salts - pharmacology Bile salts Cell Membrane - chemistry Cell Membrane - physiology Cell Membrane Permeability Culture Media Diphenylhexatriene - chemistry Fatty Acids - analysis Fatty Acids - chemistry Fluorescence Polarization Fluorescent probes Glycolipids - analysis Hydrophobic and Hydrophilic Interactions Lactobacillus casei Lactobacillus casei - chemistry Lactobacillus casei - genetics Lactobacillus casei - physiology Lipids Liposomes - chemistry Membrane Fluidity Membrane Lipids - analysis Membranes Osmotic Pressure Osmotic stress Permeability Phospholipids - analysis Protons Saline Solution, Hypertonic - pharmacology |
| title | Osmotic response in Lactobacillus casei ATCC 393: biochemical and biophysical characteristics of membrane |
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