Strand specific quantitative real-time PCR to study replication of hepatitis C virus genome

Qualitative detection of negative hepatitis C virus (HCV) RNA has been used widely to demonstrate HCV replication. However, relative quantitation of both positive and negative HCV RNA strands has never been reported for studying viral genome replication. A strand specific real-time PCR carried out i...

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Veröffentlicht in:	Journal of virological methods 2004-03, Vol.116 (1), p.103-106
Hauptverfasser:	Komurian-Pradel, Florence, Perret, Magali, Deiman, Birgit, Sodoyer, Mireille, Lotteau, Vincent, Paranhos-Baccalà, Glaucia, André, Patrice
Format:	Artikel
Sprache:	eng
Schlagworte:	5' Untranslated Regions - genetics Biological and medical sciences Fundamental and applied biological sciences. Psychology Genome, Viral HCV Hepacivirus - genetics Hepacivirus - physiology Hepatitis C - virology Hepatitis C virus Humans Liver - virology Microbiology Molecular Probes Negative and positive strand RNA Organic Chemicals Polymerase Chain Reaction - methods Quantitation Real-time PCR Reproducibility of Results RNA, Viral - analysis RNA, Viral - blood Techniques used in virology Viral Load Virology Virus Replication - genetics Virus Replication - physiology
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