Mapping and characterization of the minimal internal ribosome entry segment in the human c-myc mRNA 5′ untranslated region
The human c -myc proto-oncogene is transcribed from four alternative promoters generating transcripts with 5′ untranslated regions of various lengths. These transcripts encode two proteins, c-Myc1 and c-Myc2, from two initiation codons, CUG and AUG, respectively. We and others have previously demons...
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| Veröffentlicht in: | Oncogene 2004-01, Vol.23 (1), p.267-277 |
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| creator | Cencig, Sabrina Nanbru, Cécile Le, Shu-Yun Gueydan, Cyril Huez, Georges Kruys, Véronique |
| description | The human c
-myc
proto-oncogene is transcribed from four alternative promoters generating transcripts with 5′ untranslated regions of various lengths. These transcripts encode two proteins, c-Myc1 and c-Myc2, from two initiation codons, CUG and AUG, respectively. We and others have previously demonstrated that the region of c-
myc
transcripts between nucleotides (nt) –363 and –94 upstream from the CUG start codon contained an internal ribosome entry site leading to the cap-independent translation of c-
myc
open reading frames (ORFs). Here, we mapped a 50-nt sequence (−143 −94), which is sufficient to promote internal translation initiation of c-
myc
ORFs. Interestingly, this 50-nt element can be further dissected into two segments of 14 nt, each capable of activating internal translation initiation. We also demonstrate that this 50-nt element acts as the ribosome landing site from which the preinitiation ribosomal complex scans the mRNA until the CUG or AUG start codons. |
| doi_str_mv | 10.1038/sj.onc.1207017 |
| format | Article |
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-myc
proto-oncogene is transcribed from four alternative promoters generating transcripts with 5′ untranslated regions of various lengths. These transcripts encode two proteins, c-Myc1 and c-Myc2, from two initiation codons, CUG and AUG, respectively. We and others have previously demonstrated that the region of c-
myc
transcripts between nucleotides (nt) –363 and –94 upstream from the CUG start codon contained an internal ribosome entry site leading to the cap-independent translation of c-
myc
open reading frames (ORFs). Here, we mapped a 50-nt sequence (−143 −94), which is sufficient to promote internal translation initiation of c-
myc
ORFs. Interestingly, this 50-nt element can be further dissected into two segments of 14 nt, each capable of activating internal translation initiation. We also demonstrate that this 50-nt element acts as the ribosome landing site from which the preinitiation ribosomal complex scans the mRNA until the CUG or AUG start codons.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1207017</identifier><identifier>PMID: 14712232</identifier><identifier>CODEN: ONCNES</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>5' Untranslated Regions - chemistry ; Apoptosis ; Biological and medical sciences ; c-myc gene ; Cell Biology ; Cell cycle ; Cell growth ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Classical genetics, quantitative genetics, hybrids ; Codon, Initiator ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genes, myc ; Genetics of eukaryotes. Biological and molecular evolution ; Human Genetics ; Humans ; Internal Medicine ; Medicine ; Medicine & Public Health ; Methods, theories and miscellaneous ; Molecular and cellular biology ; Nucleic Acid Conformation ; Oncology ; original-paper ; Protein Biosynthesis ; Ribonucleic acid ; Ribosomes - metabolism ; RNA ; RNA, Messenger - chemistry</subject><ispartof>Oncogene, 2004-01, Vol.23 (1), p.267-277</ispartof><rights>Springer Nature Limited 2004</rights><rights>2004 INIST-CNRS</rights><rights>COPYRIGHT 2004 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jan 8, 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-dcbc6eaaed378f62b7c1c579497918c6a42225fd98d1e63b047cfda151764b0c3</citedby><cites>FETCH-LOGICAL-c458t-dcbc6eaaed378f62b7c1c579497918c6a42225fd98d1e63b047cfda151764b0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15673913$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14712232$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cencig, Sabrina</creatorcontrib><creatorcontrib>Nanbru, Cécile</creatorcontrib><creatorcontrib>Le, Shu-Yun</creatorcontrib><creatorcontrib>Gueydan, Cyril</creatorcontrib><creatorcontrib>Huez, Georges</creatorcontrib><creatorcontrib>Kruys, Véronique</creatorcontrib><title>Mapping and characterization of the minimal internal ribosome entry segment in the human c-myc mRNA 5′ untranslated region</title><title>Oncogene</title><addtitle>Oncogene</addtitle><addtitle>Oncogene</addtitle><description>The human c
-myc
proto-oncogene is transcribed from four alternative promoters generating transcripts with 5′ untranslated regions of various lengths. These transcripts encode two proteins, c-Myc1 and c-Myc2, from two initiation codons, CUG and AUG, respectively. We and others have previously demonstrated that the region of c-
myc
transcripts between nucleotides (nt) –363 and –94 upstream from the CUG start codon contained an internal ribosome entry site leading to the cap-independent translation of c-
myc
open reading frames (ORFs). Here, we mapped a 50-nt sequence (−143 −94), which is sufficient to promote internal translation initiation of c-
myc
ORFs. Interestingly, this 50-nt element can be further dissected into two segments of 14 nt, each capable of activating internal translation initiation. We also demonstrate that this 50-nt element acts as the ribosome landing site from which the preinitiation ribosomal complex scans the mRNA until the CUG or AUG start codons.</description><subject>5' Untranslated Regions - chemistry</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>c-myc gene</subject><subject>Cell Biology</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Codon, Initiator</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Genes, myc</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Methods, theories and miscellaneous</subject><subject>Molecular and cellular biology</subject><subject>Nucleic Acid Conformation</subject><subject>Oncology</subject><subject>original-paper</subject><subject>Protein Biosynthesis</subject><subject>Ribonucleic acid</subject><subject>Ribosomes - metabolism</subject><subject>RNA</subject><subject>RNA, Messenger - chemistry</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU2LFDEQhhtR3HH16lGCoLeeTdKdr-Ow-AWrgug5pNPpmQydZEy6D7N48Df5k_wl1u40DAgiOaSoeqoqed-qek7wmuBGXpX9OkW7JhQLTMSDakVawWvGVPuwWmHFcK1oQy-qJ6XsMcZCYfq4ugCIUMivqh8fzeHg4xaZ2CO7M9nYyWV_ayafIkoDmnYOBR99MCPyEWoRguy7VFJwyMUpH1Fx2wAR1O_x3RxMRLYOR4vCl08bxH7__IVmQE0so5lcj7Lbwvyn1aPBjMU9W-7L6tvbN1-v39c3n999uN7c1LZlcqp721nujHF9I-TAaScssUyoVglFpOWmpZSyoVeyJ443HW6FHXpDGBG87bBtLqvXp7mHnL7Prkw6-GLdOJro0ly0xFhKYP8LEkU5wBzAl3-B-zTfSVM0paJhVDYSoPUJ2prRaR-HBApYOL0L3qboBg_5DZGKcHCvOTfYnErJbtCHDMLnoyZY39mty16D3XqxGxpeLM-Yu-D6M774C8CrBTDFmnEAB6wvZ45x0aj7zVcnrkApbl0-_-cfq_8AhxvErg</recordid><startdate>20040108</startdate><enddate>20040108</enddate><creator>Cencig, Sabrina</creator><creator>Nanbru, Cécile</creator><creator>Le, Shu-Yun</creator><creator>Gueydan, Cyril</creator><creator>Huez, Georges</creator><creator>Kruys, Véronique</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040108</creationdate><title>Mapping and characterization of the minimal internal ribosome entry segment in the human c-myc mRNA 5′ untranslated region</title><author>Cencig, Sabrina ; Nanbru, Cécile ; Le, Shu-Yun ; Gueydan, Cyril ; Huez, Georges ; Kruys, Véronique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-dcbc6eaaed378f62b7c1c579497918c6a42225fd98d1e63b047cfda151764b0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>5' Untranslated Regions - chemistry</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>c-myc gene</topic><topic>Cell Biology</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Codon, Initiator</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Genes, myc</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Methods, theories and miscellaneous</topic><topic>Molecular and cellular biology</topic><topic>Nucleic Acid Conformation</topic><topic>Oncology</topic><topic>original-paper</topic><topic>Protein Biosynthesis</topic><topic>Ribonucleic acid</topic><topic>Ribosomes - metabolism</topic><topic>RNA</topic><topic>RNA, Messenger - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cencig, Sabrina</creatorcontrib><creatorcontrib>Nanbru, Cécile</creatorcontrib><creatorcontrib>Le, Shu-Yun</creatorcontrib><creatorcontrib>Gueydan, Cyril</creatorcontrib><creatorcontrib>Huez, Georges</creatorcontrib><creatorcontrib>Kruys, Véronique</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Proquest Health and Medical Complete</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Oncogene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cencig, Sabrina</au><au>Nanbru, Cécile</au><au>Le, Shu-Yun</au><au>Gueydan, Cyril</au><au>Huez, Georges</au><au>Kruys, Véronique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mapping and characterization of the minimal internal ribosome entry segment in the human c-myc mRNA 5′ untranslated region</atitle><jtitle>Oncogene</jtitle><stitle>Oncogene</stitle><addtitle>Oncogene</addtitle><date>2004-01-08</date><risdate>2004</risdate><volume>23</volume><issue>1</issue><spage>267</spage><epage>277</epage><pages>267-277</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><coden>ONCNES</coden><abstract>The human c
-myc
proto-oncogene is transcribed from four alternative promoters generating transcripts with 5′ untranslated regions of various lengths. These transcripts encode two proteins, c-Myc1 and c-Myc2, from two initiation codons, CUG and AUG, respectively. We and others have previously demonstrated that the region of c-
myc
transcripts between nucleotides (nt) –363 and –94 upstream from the CUG start codon contained an internal ribosome entry site leading to the cap-independent translation of c-
myc
open reading frames (ORFs). Here, we mapped a 50-nt sequence (−143 −94), which is sufficient to promote internal translation initiation of c-
myc
ORFs. Interestingly, this 50-nt element can be further dissected into two segments of 14 nt, each capable of activating internal translation initiation. We also demonstrate that this 50-nt element acts as the ribosome landing site from which the preinitiation ribosomal complex scans the mRNA until the CUG or AUG start codons.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>14712232</pmid><doi>10.1038/sj.onc.1207017</doi><tpages>11</tpages></addata></record> |
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| ispartof | Oncogene, 2004-01, Vol.23 (1), p.267-277 |
| issn | 0950-9232 1476-5594 |
| language | eng |
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| source | MEDLINE; Nature; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | 5' Untranslated Regions - chemistry Apoptosis Biological and medical sciences c-myc gene Cell Biology Cell cycle Cell growth Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Classical genetics, quantitative genetics, hybrids Codon, Initiator Fundamental and applied biological sciences. Psychology Gene expression Genes, myc Genetics of eukaryotes. Biological and molecular evolution Human Genetics Humans Internal Medicine Medicine Medicine & Public Health Methods, theories and miscellaneous Molecular and cellular biology Nucleic Acid Conformation Oncology original-paper Protein Biosynthesis Ribonucleic acid Ribosomes - metabolism RNA RNA, Messenger - chemistry |
| title | Mapping and characterization of the minimal internal ribosome entry segment in the human c-myc mRNA 5′ untranslated region |
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