Construction of high-density bacterial colony arrays and patterns by the ink-jet method
We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink‐jet printers. Bacterial colony arrays with a density of 100 colonies/cm2 were obtained by directly ejecting Escherichia coli (E. coli) onto agar‐coated substrates at a rapid array...
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| Veröffentlicht in: | Biotechnology and bioengineering 2004-01, Vol.85 (1), p.29-33 |
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| container_title | Biotechnology and bioengineering |
| container_volume | 85 |
| creator | Xu, Tao Petridou, Sevastioni Lee, Eric H. Roth, Elizabeth A. Vyavahare, Narendra R. Hickman, James J. Boland, Thomas |
| description | We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink‐jet printers. Bacterial colony arrays with a density of 100 colonies/cm2 were obtained by directly ejecting Escherichia coli (E. coli) onto agar‐coated substrates at a rapid arraying speed of 880 spots per second. Adjusting the concentration of bacterial suspensions allowed single colonies of viable bacteria to be obtained. In addition, complex patterns of viable bacteria as well as bacteria density gradients were constructed using desktop printers controlled by a simple software program. © 2003 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.10768 |
| format | Article |
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Bacterial colony arrays with a density of 100 colonies/cm2 were obtained by directly ejecting Escherichia coli (E. coli) onto agar‐coated substrates at a rapid arraying speed of 880 spots per second. Adjusting the concentration of bacterial suspensions allowed single colonies of viable bacteria to be obtained. 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Psychology ; ink-jet printer ; Methods. Procedures. 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Bioeng</addtitle><description>We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink‐jet printers. Bacterial colony arrays with a density of 100 colonies/cm2 were obtained by directly ejecting Escherichia coli (E. coli) onto agar‐coated substrates at a rapid arraying speed of 880 spots per second. Adjusting the concentration of bacterial suspensions allowed single colonies of viable bacteria to be obtained. In addition, complex patterns of viable bacteria as well as bacteria density gradients were constructed using desktop printers controlled by a simple software program. © 2003 Wiley Periodicals, Inc.</description><subject>bacterial colony array</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - instrumentation</subject><subject>Biological Assay - methods</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>biosensor</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Culture Techniques - methods</subject><subject>cell density gradient</subject><subject>Cell Division - physiology</subject><subject>Colony Count, Microbial - instrumentation</subject><subject>Colony Count, Microbial - methods</subject><subject>complex pattern</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Escherichia coli - cytology</subject><subject>Escherichia coli - growth & development</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ink-jet printer</subject><subject>Methods. Procedures. Technologies</subject><subject>Others</subject><subject>Printing - instrumentation</subject><subject>Printing - methods</subject><subject>Robotics - instrumentation</subject><subject>Robotics - methods</subject><subject>Various methods and equipments</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1PFDEYB_CGYGRFD3wB0wsmHkb6Mn074kaBzQY8YPDWtJ2OW5ht17YbnW_v4K5yIp7apr_n_yR_AE4w-oARImc21OkiuDwAM4yUaBBR6BDMEEK8oUyRI_CqlPvpKSTnL8ERbgViCKkZuJunWGreuhpShKmHq_B91XQ-llBHaI2rPgczQJeGFEdocjZjgSZ2cGPq9BcLtCOsKw9DfGjufYVrX1epew1e9GYo_s3-PAZfP3-6nV82y5uLq_n5snGMUNnwVhivCLatxZZKjiiSkjqGmG85Uc5yYayySlAiuCBECddLZh0TRnQtZvQYvNvlbnL6sfWl6nUozg-DiT5ti5ZoCmSU_BdihZXkhE7w_Q66nErJvtebHNYmjxoj_Vi3nurWf-qe7Nt96Nauffck9_1O4HQPTHFm6LOJLpQnx1qOBX0MOtu5n2Hw4_Mb9cer27-rm91EKNX_-jdh8oPmggqm764vNFkuLr99WVC9oL8BQQ-kag</recordid><startdate>20040105</startdate><enddate>20040105</enddate><creator>Xu, Tao</creator><creator>Petridou, Sevastioni</creator><creator>Lee, Eric H.</creator><creator>Roth, Elizabeth A.</creator><creator>Vyavahare, Narendra R.</creator><creator>Hickman, James J.</creator><creator>Boland, Thomas</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040105</creationdate><title>Construction of high-density bacterial colony arrays and patterns by the ink-jet method</title><author>Xu, Tao ; Petridou, Sevastioni ; Lee, Eric H. ; Roth, Elizabeth A. ; Vyavahare, Narendra R. ; Hickman, James J. ; Boland, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5238-647ae921b4b1b386030883c505e4629cb67ab9b97327672297cf85bc57a7d4153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>bacterial colony array</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - instrumentation</topic><topic>Biological Assay - methods</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>biosensor</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Cell Culture Techniques - methods</topic><topic>cell density gradient</topic><topic>Cell Division - physiology</topic><topic>Colony Count, Microbial - instrumentation</topic><topic>Colony Count, Microbial - methods</topic><topic>complex pattern</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Escherichia coli - cytology</topic><topic>Escherichia coli - growth & development</topic><topic>Fundamental and applied biological sciences. 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Bioeng</addtitle><date>2004-01-05</date><risdate>2004</risdate><volume>85</volume><issue>1</issue><spage>29</spage><epage>33</epage><pages>29-33</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink‐jet printers. Bacterial colony arrays with a density of 100 colonies/cm2 were obtained by directly ejecting Escherichia coli (E. coli) onto agar‐coated substrates at a rapid arraying speed of 880 spots per second. Adjusting the concentration of bacterial suspensions allowed single colonies of viable bacteria to be obtained. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | bacterial colony array Biological and medical sciences Biological Assay - instrumentation Biological Assay - methods Biosensing Techniques - instrumentation Biosensing Techniques - methods biosensor Biotechnology Cell Culture Techniques - instrumentation Cell Culture Techniques - methods cell density gradient Cell Division - physiology Colony Count, Microbial - instrumentation Colony Count, Microbial - methods complex pattern Equipment Design Equipment Failure Analysis Escherichia coli - cytology Escherichia coli - growth & development Fundamental and applied biological sciences. Psychology ink-jet printer Methods. Procedures. Technologies Others Printing - instrumentation Printing - methods Robotics - instrumentation Robotics - methods Various methods and equipments |
| title | Construction of high-density bacterial colony arrays and patterns by the ink-jet method |
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