A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1

For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline pho...

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Veröffentlicht in:Biosensors & bioelectronics 2004-02, Vol.19 (7), p.685-692
Hauptverfasser: Wen, Ji-Kai, Zhang, Xian-En, Cheng, Zhi, Liu, Hong, Zhou, Ya-Feng, Zhang, Zhi-Ping, Yang, Ji-Hong, Deng, Jiao-Yu
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container_end_page 692
container_issue 7
container_start_page 685
container_title Biosensors & bioelectronics
container_volume 19
creator Wen, Ji-Kai
Zhang, Xian-En
Cheng, Zhi
Liu, Hong
Zhou, Ya-Feng
Zhang, Zhi-Ping
Yang, Ji-Hong
Deng, Jiao-Yu
description For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline phosphatase (Av-AP) indicator system, was developed. After pretreatment of infected blood samples and reverse transcription of the RNA virus genome, PCR was performed in a single tube by using the outer primer pairs. Second round nested multiplex PCR was performed on the DNA chip, on which the primers array had already been prepared. During the arrayed anchored multiplex PCR, 5[ N-( N-biotinylaminocaproyl)-ε-3-aminoallyl]-2-deoxy-uridine-5-triphosphate (biotin-11-dUTP) was incorporated into the extended DNA chains in order to bind avidin alkaline phosphatase via avidin and biotin. To produce purple precipitates on the chips, the enzyme substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) was used in conjunction with the enhancer, nitro blue tetrazolium (NBT). Blood samples containing the three viruses were tested using this DNA chip and about 1 pg of specific viral DNA fragments were detected on the chip wells after nested PCR.
doi_str_mv 10.1016/S0956-5663(03)00264-1
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Blood samples containing the three viruses were tested using this DNA chip and about 1 pg of specific viral DNA fragments were detected on the chip wells after nested PCR.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(03)00264-1</identifier><identifier>PMID: 14709386</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Alkaline phosphatase ; avidin ; Biological and medical sciences ; Biosensors ; Biotechnology ; biotin ; Biotin and avidin system ; Colorimetry - instrumentation ; Colorimetry - methods ; DNA chip ; DNA chips ; DNA, Viral - blood ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Hepacivirus - genetics ; Hepacivirus - isolation &amp; purification ; Hepatitis B - blood ; Hepatitis B - genetics ; Hepatitis B virus ; Hepatitis B virus (HBV) ; Hepatitis B virus - genetics ; Hepatitis B virus - isolation &amp; purification ; Hepatitis C - blood ; Hepatitis C - genetics ; Hepatitis C virus ; Hepatitis C virus (HCV) ; HIV Infections - blood ; HIV Infections - genetics ; HIV-1 - genetics ; HIV-1 - isolation &amp; purification ; Human immunodeficiency virus 1 ; Human immunodeficiency virus type-1 (HIV-1) ; Humans ; Methods. Procedures. Technologies ; Multiplex nested array anchored primer PCR ; Oligonucleotide Array Sequence Analysis - instrumentation ; Oligonucleotide Array Sequence Analysis - methods ; Polymerase Chain Reaction - instrumentation ; Polymerase Chain Reaction - methods ; Reproducibility of Results ; Sensitivity and Specificity ; Various methods and equipments</subject><ispartof>Biosensors &amp; bioelectronics, 2004-02, Vol.19 (7), p.685-692</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-875578e2713b18b6ac54154ff315f8541741fcd666ec1dcdee9e5e483aa6dbdc3</citedby><cites>FETCH-LOGICAL-c459t-875578e2713b18b6ac54154ff315f8541741fcd666ec1dcdee9e5e483aa6dbdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0956-5663(03)00264-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15474776$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14709386$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wen, Ji-Kai</creatorcontrib><creatorcontrib>Zhang, Xian-En</creatorcontrib><creatorcontrib>Cheng, Zhi</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Zhou, Ya-Feng</creatorcontrib><creatorcontrib>Zhang, Zhi-Ping</creatorcontrib><creatorcontrib>Yang, Ji-Hong</creatorcontrib><creatorcontrib>Deng, Jiao-Yu</creatorcontrib><title>A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1</title><title>Biosensors &amp; bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline phosphatase (Av-AP) indicator system, was developed. 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Psychology</subject><subject>Hepacivirus - genetics</subject><subject>Hepacivirus - isolation &amp; purification</subject><subject>Hepatitis B - blood</subject><subject>Hepatitis B - genetics</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus (HBV)</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - isolation &amp; purification</subject><subject>Hepatitis C - blood</subject><subject>Hepatitis C - genetics</subject><subject>Hepatitis C virus</subject><subject>Hepatitis C virus (HCV)</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - genetics</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - isolation &amp; purification</subject><subject>Human immunodeficiency virus 1</subject><subject>Human immunodeficiency virus type-1 (HIV-1)</subject><subject>Humans</subject><subject>Methods. Procedures. Technologies</subject><subject>Multiplex nested array anchored primer PCR</subject><subject>Oligonucleotide Array Sequence Analysis - instrumentation</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Polymerase Chain Reaction - instrumentation</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVuLFDEQhRtR3NnVn6DkRVGwNdW59pOM4xUWfVCfQyapMJG-bdK9MOCP357txvVtIVCh-E5Vck5RPAP6FijIdz9pLWQppGSvKHtNaSV5CQ-KDWjFSl4x8bDY_EPOivOc_1BKFdT0cXEGXNGaabkp_m7JdcyTbcjH71viDnEgoU8kx3ZqRtthP2XicUQ3xr4jfSAHHOwYx5jJh1mZpvzmv9ZuaRHbeXKYWtuR2LZT13sM0UXs3HEFxuOAJTwpHgXbZHy61ovi9-dPv3Zfy8sfX77ttpel46IeS62EUBorBWwPei-tExwED4GBCHq-Kw7BeSklOvDOI9YokGtmrfR779hF8XKZO6T-asI8mjZmh02zfNBoSrUEqu8Foa4qqCo2g2IBXepzThjMkGJr09EANad8zG0-5mS-ofM55WNg1j1fF0z7Fv2dag1kBl6sgM3ONiHZzsV8xwmuuFIn7v3C4ezbdcRk8q3B6GOawzK-j_c85QbuTa2r</recordid><startdate>20040215</startdate><enddate>20040215</enddate><creator>Wen, Ji-Kai</creator><creator>Zhang, Xian-En</creator><creator>Cheng, Zhi</creator><creator>Liu, Hong</creator><creator>Zhou, Ya-Feng</creator><creator>Zhang, Zhi-Ping</creator><creator>Yang, Ji-Hong</creator><creator>Deng, Jiao-Yu</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040215</creationdate><title>A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1</title><author>Wen, Ji-Kai ; Zhang, Xian-En ; Cheng, Zhi ; Liu, Hong ; Zhou, Ya-Feng ; Zhang, Zhi-Ping ; Yang, Ji-Hong ; Deng, Jiao-Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-875578e2713b18b6ac54154ff315f8541741fcd666ec1dcdee9e5e483aa6dbdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alkaline phosphatase</topic><topic>avidin</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>biotin</topic><topic>Biotin and avidin system</topic><topic>Colorimetry - instrumentation</topic><topic>Colorimetry - methods</topic><topic>DNA chip</topic><topic>DNA chips</topic><topic>DNA, Viral - blood</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hepacivirus - genetics</topic><topic>Hepacivirus - isolation &amp; purification</topic><topic>Hepatitis B - blood</topic><topic>Hepatitis B - genetics</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus (HBV)</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - isolation &amp; purification</topic><topic>Hepatitis C - blood</topic><topic>Hepatitis C - genetics</topic><topic>Hepatitis C virus</topic><topic>Hepatitis C virus (HCV)</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - genetics</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - isolation &amp; purification</topic><topic>Human immunodeficiency virus 1</topic><topic>Human immunodeficiency virus type-1 (HIV-1)</topic><topic>Humans</topic><topic>Methods. Procedures. Technologies</topic><topic>Multiplex nested array anchored primer PCR</topic><topic>Oligonucleotide Array Sequence Analysis - instrumentation</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Polymerase Chain Reaction - instrumentation</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wen, Ji-Kai</creatorcontrib><creatorcontrib>Zhang, Xian-En</creatorcontrib><creatorcontrib>Cheng, Zhi</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Zhou, Ya-Feng</creatorcontrib><creatorcontrib>Zhang, Zhi-Ping</creatorcontrib><creatorcontrib>Yang, Ji-Hong</creatorcontrib><creatorcontrib>Deng, Jiao-Yu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors &amp; bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wen, Ji-Kai</au><au>Zhang, Xian-En</au><au>Cheng, Zhi</au><au>Liu, Hong</au><au>Zhou, Ya-Feng</au><au>Zhang, Zhi-Ping</au><au>Yang, Ji-Hong</au><au>Deng, Jiao-Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1</atitle><jtitle>Biosensors &amp; bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2004-02-15</date><risdate>2004</risdate><volume>19</volume><issue>7</issue><spage>685</spage><epage>692</epage><pages>685-692</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline phosphatase (Av-AP) indicator system, was developed. After pretreatment of infected blood samples and reverse transcription of the RNA virus genome, PCR was performed in a single tube by using the outer primer pairs. Second round nested multiplex PCR was performed on the DNA chip, on which the primers array had already been prepared. During the arrayed anchored multiplex PCR, 5[ N-( N-biotinylaminocaproyl)-ε-3-aminoallyl]-2-deoxy-uridine-5-triphosphate (biotin-11-dUTP) was incorporated into the extended DNA chains in order to bind avidin alkaline phosphatase via avidin and biotin. To produce purple precipitates on the chips, the enzyme substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) was used in conjunction with the enhancer, nitro blue tetrazolium (NBT). Blood samples containing the three viruses were tested using this DNA chip and about 1 pg of specific viral DNA fragments were detected on the chip wells after nested PCR.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>14709386</pmid><doi>10.1016/S0956-5663(03)00264-1</doi><tpages>8</tpages></addata></record>
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subjects Alkaline phosphatase
avidin
Biological and medical sciences
Biosensors
Biotechnology
biotin
Biotin and avidin system
Colorimetry - instrumentation
Colorimetry - methods
DNA chip
DNA chips
DNA, Viral - blood
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Hepacivirus - genetics
Hepacivirus - isolation & purification
Hepatitis B - blood
Hepatitis B - genetics
Hepatitis B virus
Hepatitis B virus (HBV)
Hepatitis B virus - genetics
Hepatitis B virus - isolation & purification
Hepatitis C - blood
Hepatitis C - genetics
Hepatitis C virus
Hepatitis C virus (HCV)
HIV Infections - blood
HIV Infections - genetics
HIV-1 - genetics
HIV-1 - isolation & purification
Human immunodeficiency virus 1
Human immunodeficiency virus type-1 (HIV-1)
Humans
Methods. Procedures. Technologies
Multiplex nested array anchored primer PCR
Oligonucleotide Array Sequence Analysis - instrumentation
Oligonucleotide Array Sequence Analysis - methods
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Reproducibility of Results
Sensitivity and Specificity
Various methods and equipments
title A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
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