A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline pho...
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Veröffentlicht in: | Biosensors & bioelectronics 2004-02, Vol.19 (7), p.685-692 |
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creator | Wen, Ji-Kai Zhang, Xian-En Cheng, Zhi Liu, Hong Zhou, Ya-Feng Zhang, Zhi-Ping Yang, Ji-Hong Deng, Jiao-Yu |
description | For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline phosphatase (Av-AP) indicator system, was developed. After pretreatment of infected blood samples and reverse transcription of the RNA virus genome, PCR was performed in a single tube by using the outer primer pairs. Second round nested multiplex PCR was performed on the DNA chip, on which the primers array had already been prepared. During the arrayed anchored multiplex PCR, 5[
N-(
N-biotinylaminocaproyl)-ε-3-aminoallyl]-2-deoxy-uridine-5-triphosphate (biotin-11-dUTP) was incorporated into the extended DNA chains in order to bind avidin alkaline phosphatase via avidin and biotin. To produce purple precipitates on the chips, the enzyme substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) was used in conjunction with the enhancer, nitro blue tetrazolium (NBT). Blood samples containing the three viruses were tested using this DNA chip and about 1
pg of specific viral DNA fragments were detected on the chip wells after nested PCR. |
doi_str_mv | 10.1016/S0956-5663(03)00264-1 |
format | Article |
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N-(
N-biotinylaminocaproyl)-ε-3-aminoallyl]-2-deoxy-uridine-5-triphosphate (biotin-11-dUTP) was incorporated into the extended DNA chains in order to bind avidin alkaline phosphatase via avidin and biotin. To produce purple precipitates on the chips, the enzyme substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) was used in conjunction with the enhancer, nitro blue tetrazolium (NBT). Blood samples containing the three viruses were tested using this DNA chip and about 1
pg of specific viral DNA fragments were detected on the chip wells after nested PCR.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(03)00264-1</identifier><identifier>PMID: 14709386</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Alkaline phosphatase ; avidin ; Biological and medical sciences ; Biosensors ; Biotechnology ; biotin ; Biotin and avidin system ; Colorimetry - instrumentation ; Colorimetry - methods ; DNA chip ; DNA chips ; DNA, Viral - blood ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Hepacivirus - genetics ; Hepacivirus - isolation & purification ; Hepatitis B - blood ; Hepatitis B - genetics ; Hepatitis B virus ; Hepatitis B virus (HBV) ; Hepatitis B virus - genetics ; Hepatitis B virus - isolation & purification ; Hepatitis C - blood ; Hepatitis C - genetics ; Hepatitis C virus ; Hepatitis C virus (HCV) ; HIV Infections - blood ; HIV Infections - genetics ; HIV-1 - genetics ; HIV-1 - isolation & purification ; Human immunodeficiency virus 1 ; Human immunodeficiency virus type-1 (HIV-1) ; Humans ; Methods. Procedures. Technologies ; Multiplex nested array anchored primer PCR ; Oligonucleotide Array Sequence Analysis - instrumentation ; Oligonucleotide Array Sequence Analysis - methods ; Polymerase Chain Reaction - instrumentation ; Polymerase Chain Reaction - methods ; Reproducibility of Results ; Sensitivity and Specificity ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2004-02, Vol.19 (7), p.685-692</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-875578e2713b18b6ac54154ff315f8541741fcd666ec1dcdee9e5e483aa6dbdc3</citedby><cites>FETCH-LOGICAL-c459t-875578e2713b18b6ac54154ff315f8541741fcd666ec1dcdee9e5e483aa6dbdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0956-5663(03)00264-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15474776$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14709386$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wen, Ji-Kai</creatorcontrib><creatorcontrib>Zhang, Xian-En</creatorcontrib><creatorcontrib>Cheng, Zhi</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Zhou, Ya-Feng</creatorcontrib><creatorcontrib>Zhang, Zhi-Ping</creatorcontrib><creatorcontrib>Yang, Ji-Hong</creatorcontrib><creatorcontrib>Deng, Jiao-Yu</creatorcontrib><title>A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline phosphatase (Av-AP) indicator system, was developed. After pretreatment of infected blood samples and reverse transcription of the RNA virus genome, PCR was performed in a single tube by using the outer primer pairs. Second round nested multiplex PCR was performed on the DNA chip, on which the primers array had already been prepared. During the arrayed anchored multiplex PCR, 5[
N-(
N-biotinylaminocaproyl)-ε-3-aminoallyl]-2-deoxy-uridine-5-triphosphate (biotin-11-dUTP) was incorporated into the extended DNA chains in order to bind avidin alkaline phosphatase via avidin and biotin. To produce purple precipitates on the chips, the enzyme substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) was used in conjunction with the enhancer, nitro blue tetrazolium (NBT). Blood samples containing the three viruses were tested using this DNA chip and about 1
pg of specific viral DNA fragments were detected on the chip wells after nested PCR.</description><subject>Alkaline phosphatase</subject><subject>avidin</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>biotin</subject><subject>Biotin and avidin system</subject><subject>Colorimetry - instrumentation</subject><subject>Colorimetry - methods</subject><subject>DNA chip</subject><subject>DNA chips</subject><subject>DNA, Viral - blood</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hepacivirus - genetics</subject><subject>Hepacivirus - isolation & purification</subject><subject>Hepatitis B - blood</subject><subject>Hepatitis B - genetics</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus (HBV)</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - isolation & purification</subject><subject>Hepatitis C - blood</subject><subject>Hepatitis C - genetics</subject><subject>Hepatitis C virus</subject><subject>Hepatitis C virus (HCV)</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - genetics</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - isolation & purification</subject><subject>Human immunodeficiency virus 1</subject><subject>Human immunodeficiency virus type-1 (HIV-1)</subject><subject>Humans</subject><subject>Methods. Procedures. Technologies</subject><subject>Multiplex nested array anchored primer PCR</subject><subject>Oligonucleotide Array Sequence Analysis - instrumentation</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Polymerase Chain Reaction - instrumentation</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVuLFDEQhRtR3NnVn6DkRVGwNdW59pOM4xUWfVCfQyapMJG-bdK9MOCP357txvVtIVCh-E5Vck5RPAP6FijIdz9pLWQppGSvKHtNaSV5CQ-KDWjFSl4x8bDY_EPOivOc_1BKFdT0cXEGXNGaabkp_m7JdcyTbcjH71viDnEgoU8kx3ZqRtthP2XicUQ3xr4jfSAHHOwYx5jJh1mZpvzmv9ZuaRHbeXKYWtuR2LZT13sM0UXs3HEFxuOAJTwpHgXbZHy61ovi9-dPv3Zfy8sfX77ttpel46IeS62EUBorBWwPei-tExwED4GBCHq-Kw7BeSklOvDOI9YokGtmrfR779hF8XKZO6T-asI8mjZmh02zfNBoSrUEqu8Foa4qqCo2g2IBXepzThjMkGJr09EANad8zG0-5mS-ofM55WNg1j1fF0z7Fv2dag1kBl6sgM3ONiHZzsV8xwmuuFIn7v3C4ezbdcRk8q3B6GOawzK-j_c85QbuTa2r</recordid><startdate>20040215</startdate><enddate>20040215</enddate><creator>Wen, Ji-Kai</creator><creator>Zhang, Xian-En</creator><creator>Cheng, Zhi</creator><creator>Liu, Hong</creator><creator>Zhou, Ya-Feng</creator><creator>Zhang, Zhi-Ping</creator><creator>Yang, Ji-Hong</creator><creator>Deng, Jiao-Yu</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040215</creationdate><title>A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1</title><author>Wen, Ji-Kai ; Zhang, Xian-En ; Cheng, Zhi ; Liu, Hong ; Zhou, Ya-Feng ; Zhang, Zhi-Ping ; Yang, Ji-Hong ; Deng, Jiao-Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-875578e2713b18b6ac54154ff315f8541741fcd666ec1dcdee9e5e483aa6dbdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alkaline phosphatase</topic><topic>avidin</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>biotin</topic><topic>Biotin and avidin system</topic><topic>Colorimetry - instrumentation</topic><topic>Colorimetry - methods</topic><topic>DNA chip</topic><topic>DNA chips</topic><topic>DNA, Viral - blood</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hepacivirus - genetics</topic><topic>Hepacivirus - isolation & purification</topic><topic>Hepatitis B - blood</topic><topic>Hepatitis B - genetics</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus (HBV)</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - isolation & purification</topic><topic>Hepatitis C - blood</topic><topic>Hepatitis C - genetics</topic><topic>Hepatitis C virus</topic><topic>Hepatitis C virus (HCV)</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - genetics</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - isolation & purification</topic><topic>Human immunodeficiency virus 1</topic><topic>Human immunodeficiency virus type-1 (HIV-1)</topic><topic>Humans</topic><topic>Methods. Procedures. Technologies</topic><topic>Multiplex nested array anchored primer PCR</topic><topic>Oligonucleotide Array Sequence Analysis - instrumentation</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Polymerase Chain Reaction - instrumentation</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wen, Ji-Kai</creatorcontrib><creatorcontrib>Zhang, Xian-En</creatorcontrib><creatorcontrib>Cheng, Zhi</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Zhou, Ya-Feng</creatorcontrib><creatorcontrib>Zhang, Zhi-Ping</creatorcontrib><creatorcontrib>Yang, Ji-Hong</creatorcontrib><creatorcontrib>Deng, Jiao-Yu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wen, Ji-Kai</au><au>Zhang, Xian-En</au><au>Cheng, Zhi</au><au>Liu, Hong</au><au>Zhou, Ya-Feng</au><au>Zhang, Zhi-Ping</au><au>Yang, Ji-Hong</au><au>Deng, Jiao-Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2004-02-15</date><risdate>2004</risdate><volume>19</volume><issue>7</issue><spage>685</spage><epage>692</epage><pages>685-692</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>For the simultaneously visual detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type-1 (HIV-1), a qualitative DNA chip method, combining multiplex and nested polymerase chain reaction (PCR) with arrayed anchored primer PCR and a biotin-avidin alkaline phosphatase (Av-AP) indicator system, was developed. After pretreatment of infected blood samples and reverse transcription of the RNA virus genome, PCR was performed in a single tube by using the outer primer pairs. Second round nested multiplex PCR was performed on the DNA chip, on which the primers array had already been prepared. During the arrayed anchored multiplex PCR, 5[
N-(
N-biotinylaminocaproyl)-ε-3-aminoallyl]-2-deoxy-uridine-5-triphosphate (biotin-11-dUTP) was incorporated into the extended DNA chains in order to bind avidin alkaline phosphatase via avidin and biotin. To produce purple precipitates on the chips, the enzyme substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) was used in conjunction with the enhancer, nitro blue tetrazolium (NBT). Blood samples containing the three viruses were tested using this DNA chip and about 1
pg of specific viral DNA fragments were detected on the chip wells after nested PCR.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>14709386</pmid><doi>10.1016/S0956-5663(03)00264-1</doi><tpages>8</tpages></addata></record> |
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subjects | Alkaline phosphatase avidin Biological and medical sciences Biosensors Biotechnology biotin Biotin and avidin system Colorimetry - instrumentation Colorimetry - methods DNA chip DNA chips DNA, Viral - blood Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Hepacivirus - genetics Hepacivirus - isolation & purification Hepatitis B - blood Hepatitis B - genetics Hepatitis B virus Hepatitis B virus (HBV) Hepatitis B virus - genetics Hepatitis B virus - isolation & purification Hepatitis C - blood Hepatitis C - genetics Hepatitis C virus Hepatitis C virus (HCV) HIV Infections - blood HIV Infections - genetics HIV-1 - genetics HIV-1 - isolation & purification Human immunodeficiency virus 1 Human immunodeficiency virus type-1 (HIV-1) Humans Methods. Procedures. Technologies Multiplex nested array anchored primer PCR Oligonucleotide Array Sequence Analysis - instrumentation Oligonucleotide Array Sequence Analysis - methods Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods Reproducibility of Results Sensitivity and Specificity Various methods and equipments |
title | A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1 |
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