Intermediate filaments in the inner ear of normal and experimentally damaged guinea pigs
The hypothesis that proteins known to occur in glial cells in the central nervous system may be present in inner-ear supporting cells was investigated. Immunocytochemical techniques were used to look for the existence of two classes of intermediate filaments, vimentin and glial fibrillary acidic pro...
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| Veröffentlicht in: | Hearing research 1990-08, Vol.47 (1), p.1-16 |
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| creator | Oesterle, Elizabeth C Sarthy, P.Vijay Rubel, Edwin W |
| description | The hypothesis that proteins known to occur in glial cells in the central nervous system may be present in inner-ear supporting cells was investigated. Immunocytochemical techniques were used to look for the existence of two classes of intermediate filaments, vimentin and glial fibrillary acidic protein (GFAP), in cellular elements of the inner-ear epithelium in normal and experimentally damaged guinea-pig cochleas. Vimentin is present in two types of supporting cells in the normal organ of Corti: Deiters' cells and inner pillar cells. Differences in intensity and distribution of vimentin immunostaining are observed across the three rows of Deiters' cells. GFAP immunoreactivity was not detected in any supporting-cell type in the organ. Cochlear hair cells were not labeled for either GFAP or vimentin. Following hair-cell destruction by exposure to noise or the administration of aminoglycosides, GFAP and vimentin are not present in phalangeal scars replacing lost hair cells. |
| doi_str_mv | 10.1016/0378-5955(90)90162-I |
| format | Article |
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Immunocytochemical techniques were used to look for the existence of two classes of intermediate filaments, vimentin and glial fibrillary acidic protein (GFAP), in cellular elements of the inner-ear epithelium in normal and experimentally damaged guinea-pig cochleas. Vimentin is present in two types of supporting cells in the normal organ of Corti: Deiters' cells and inner pillar cells. Differences in intensity and distribution of vimentin immunostaining are observed across the three rows of Deiters' cells. GFAP immunoreactivity was not detected in any supporting-cell type in the organ. Cochlear hair cells were not labeled for either GFAP or vimentin. Following hair-cell destruction by exposure to noise or the administration of aminoglycosides, GFAP and vimentin are not present in phalangeal scars replacing lost hair cells.</description><identifier>ISSN: 0378-5955</identifier><identifier>EISSN: 1878-5891</identifier><identifier>DOI: 10.1016/0378-5955(90)90162-I</identifier><identifier>PMID: 2228788</identifier><identifier>CODEN: HERED3</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cochlea ; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation ; Ear, Inner - injuries ; Ear, Inner - metabolism ; Ear, Inner - ultrastructure ; Female ; Fundamental and applied biological sciences. Psychology ; Glial Fibrillary Acidic Protein - metabolism ; Guinea Pigs ; Hair Cells, Auditory - metabolism ; Hair Cells, Auditory - ultrastructure ; Immunohistochemistry ; Inner-ear supporting cells ; Intermediate filaments ; Intermediate Filaments - metabolism ; Intermediate Filaments - ultrastructure ; Male ; Neuroglia - metabolism ; Neuroglia - ultrastructure ; Sound- and drug-damaged inner ear ; Vertebrates: nervous system and sense organs ; Vimentin - metabolism</subject><ispartof>Hearing research, 1990-08, Vol.47 (1), p.1-16</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-9af5e2a375099cf9a193cd215ffdc0ad2ae120fefe660213de0e5c0eaee32b043</citedby><cites>FETCH-LOGICAL-c469t-9af5e2a375099cf9a193cd215ffdc0ad2ae120fefe660213de0e5c0eaee32b043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-5955(90)90162-I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19323273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2228788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oesterle, Elizabeth C</creatorcontrib><creatorcontrib>Sarthy, P.Vijay</creatorcontrib><creatorcontrib>Rubel, Edwin W</creatorcontrib><title>Intermediate filaments in the inner ear of normal and experimentally damaged guinea pigs</title><title>Hearing research</title><addtitle>Hear Res</addtitle><description>The hypothesis that proteins known to occur in glial cells in the central nervous system may be present in inner-ear supporting cells was investigated. Immunocytochemical techniques were used to look for the existence of two classes of intermediate filaments, vimentin and glial fibrillary acidic protein (GFAP), in cellular elements of the inner-ear epithelium in normal and experimentally damaged guinea-pig cochleas. Vimentin is present in two types of supporting cells in the normal organ of Corti: Deiters' cells and inner pillar cells. Differences in intensity and distribution of vimentin immunostaining are observed across the three rows of Deiters' cells. GFAP immunoreactivity was not detected in any supporting-cell type in the organ. Cochlear hair cells were not labeled for either GFAP or vimentin. Following hair-cell destruction by exposure to noise or the administration of aminoglycosides, GFAP and vimentin are not present in phalangeal scars replacing lost hair cells.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cochlea</subject><subject>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</subject><subject>Ear, Inner - injuries</subject><subject>Ear, Inner - metabolism</subject><subject>Ear, Inner - ultrastructure</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Guinea Pigs</subject><subject>Hair Cells, Auditory - metabolism</subject><subject>Hair Cells, Auditory - ultrastructure</subject><subject>Immunohistochemistry</subject><subject>Inner-ear supporting cells</subject><subject>Intermediate filaments</subject><subject>Intermediate Filaments - metabolism</subject><subject>Intermediate Filaments - ultrastructure</subject><subject>Male</subject><subject>Neuroglia - metabolism</subject><subject>Neuroglia - ultrastructure</subject><subject>Sound- and drug-damaged inner ear</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Vimentin - metabolism</subject><issn>0378-5955</issn><issn>1878-5891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd9rFDEQx4NY6rX6HyjkRakPWyfJZXfzUpDij4NCXyr4FqbJ5IzsZs9kT-x_b9Y76ps-Dcx85kvmE8ZeCrgUINp3oLq-0UbrCwNvTe3IZvOErUS_tHsjnrLVI_KMnZXyHUBotZan7FRKWbF-xb5u0kx5JB9xJh7igCOlufCY-PyNakmUOWHmU-BpyiMOHJPn9GtHOS4oDsMD9zjiljzf7mMi5Lu4Lc_ZScCh0ItjPWdfPn64u_7c3Nx-2ly_v2ncujVzYzBokqg6Dca4YFAY5bwUOgTvAL1EEhICBWpbkEJ5AtIOCImUvIe1OmdvDrm7PP3YU5ntGIujYcBE077YHqDXpuv-CwptWqnbBVwfQJenUjIFu6unYn6wAuxi3i5a7aLVGrB_zNtNXXt1zN_fV5-PS0fVdf76OMficAgZk4vlb7ZRUslOVe7qwFG19jNStsVFSq5-USY3Wz_Ffz_kNw4CoLc</recordid><startdate>19900801</startdate><enddate>19900801</enddate><creator>Oesterle, Elizabeth C</creator><creator>Sarthy, P.Vijay</creator><creator>Rubel, Edwin W</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>19900801</creationdate><title>Intermediate filaments in the inner ear of normal and experimentally damaged guinea pigs</title><author>Oesterle, Elizabeth C ; Sarthy, P.Vijay ; Rubel, Edwin W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-9af5e2a375099cf9a193cd215ffdc0ad2ae120fefe660213de0e5c0eaee32b043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cochlea</topic><topic>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</topic><topic>Ear, Inner - injuries</topic><topic>Ear, Inner - metabolism</topic><topic>Ear, Inner - ultrastructure</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Guinea Pigs</topic><topic>Hair Cells, Auditory - metabolism</topic><topic>Hair Cells, Auditory - ultrastructure</topic><topic>Immunohistochemistry</topic><topic>Inner-ear supporting cells</topic><topic>Intermediate filaments</topic><topic>Intermediate Filaments - metabolism</topic><topic>Intermediate Filaments - ultrastructure</topic><topic>Male</topic><topic>Neuroglia - metabolism</topic><topic>Neuroglia - ultrastructure</topic><topic>Sound- and drug-damaged inner ear</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oesterle, Elizabeth C</creatorcontrib><creatorcontrib>Sarthy, P.Vijay</creatorcontrib><creatorcontrib>Rubel, Edwin W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Hearing research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oesterle, Elizabeth C</au><au>Sarthy, P.Vijay</au><au>Rubel, Edwin W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intermediate filaments in the inner ear of normal and experimentally damaged guinea pigs</atitle><jtitle>Hearing research</jtitle><addtitle>Hear Res</addtitle><date>1990-08-01</date><risdate>1990</risdate><volume>47</volume><issue>1</issue><spage>1</spage><epage>16</epage><pages>1-16</pages><issn>0378-5955</issn><eissn>1878-5891</eissn><coden>HERED3</coden><abstract>The hypothesis that proteins known to occur in glial cells in the central nervous system may be present in inner-ear supporting cells was investigated. Immunocytochemical techniques were used to look for the existence of two classes of intermediate filaments, vimentin and glial fibrillary acidic protein (GFAP), in cellular elements of the inner-ear epithelium in normal and experimentally damaged guinea-pig cochleas. Vimentin is present in two types of supporting cells in the normal organ of Corti: Deiters' cells and inner pillar cells. Differences in intensity and distribution of vimentin immunostaining are observed across the three rows of Deiters' cells. GFAP immunoreactivity was not detected in any supporting-cell type in the organ. Cochlear hair cells were not labeled for either GFAP or vimentin. Following hair-cell destruction by exposure to noise or the administration of aminoglycosides, GFAP and vimentin are not present in phalangeal scars replacing lost hair cells.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2228788</pmid><doi>10.1016/0378-5955(90)90162-I</doi><tpages>16</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Biological and medical sciences Cochlea Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation Ear, Inner - injuries Ear, Inner - metabolism Ear, Inner - ultrastructure Female Fundamental and applied biological sciences. Psychology Glial Fibrillary Acidic Protein - metabolism Guinea Pigs Hair Cells, Auditory - metabolism Hair Cells, Auditory - ultrastructure Immunohistochemistry Inner-ear supporting cells Intermediate filaments Intermediate Filaments - metabolism Intermediate Filaments - ultrastructure Male Neuroglia - metabolism Neuroglia - ultrastructure Sound- and drug-damaged inner ear Vertebrates: nervous system and sense organs Vimentin - metabolism |
| title | Intermediate filaments in the inner ear of normal and experimentally damaged guinea pigs |
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